Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex

Wang, Jingwen; Liu, Jiahui; Ye, Miaoman; Liu, Feng; Wu, Su; Huang, Junjiu; Shi, Guang

doi:10.1186/s13287-020-01800-w

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…DDX56 is a component of free nucleoplasm 65S preribosomal particles, which may participate in the assembly of the 60S ribosomal subunit. DDX56 has also been reported to regulate ribosome assembly through its association with the Oct4 and Sox2 complex, which is necessary to maintain embryonic stem cells (ESCs) proliferation [ 91 ]. DDX56 is upregulated in osteosarcoma and colorectal cancer ( Table 1 ) [ 92 , 93 , 94 ].…”

Section: The Role Of Rna Helicases In Regulation Of Cell Cycle Progressionmentioning

confidence: 99%

DEAD-Box RNA Helicases in Cell Cycle Control and Clinical Therapy

Zhang

2021

Cells

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Cell cycle is regulated through numerous signaling pathways that determine whether cells will proliferate, remain quiescent, arrest, or undergo apoptosis. Abnormal cell cycle regulation has been linked to many diseases. Thus, there is an urgent need to understand the diverse molecular mechanisms of how the cell cycle is controlled. RNA helicases constitute a large family of proteins with functions in all aspects of RNA metabolism, including unwinding or annealing of RNA molecules to regulate pre-mRNA, rRNA and miRNA processing, clamping protein complexes on RNA, or remodeling ribonucleoprotein complexes, to regulate gene expression. RNA helicases also regulate the activity of specific proteins through direct interaction. Abnormal expression of RNA helicases has been associated with different diseases, including cancer, neurological disorders, aging, and autosomal dominant polycystic kidney disease (ADPKD) via regulation of a diverse range of cellular processes such as cell proliferation, cell cycle arrest, and apoptosis. Recent studies showed that RNA helicases participate in the regulation of the cell cycle progression at each cell cycle phase, including G1-S transition, S phase, G2-M transition, mitosis, and cytokinesis. In this review, we discuss the essential roles and mechanisms of RNA helicases in the regulation of the cell cycle at different phases. For that, RNA helicases provide a rich source of targets for the development of therapeutic or prophylactic drugs. We also discuss the different targeting strategies against RNA helicases, the different types of compounds explored, the proposed inhibitory mechanisms of the compounds on specific RNA helicases, and the therapeutic potential of these compounds in the treatment of various disorders.

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Section: The Role Of Rna Helicases In Regulation Of Cell Cycle Progressionmentioning

confidence: 99%

DEAD-Box RNA Helicases in Cell Cycle Control and Clinical Therapy

Zhang

2021

Cells

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“…DDX56 is located on chromosome 7 and regulates diverse RNA metabolic processes, including transcription, translation, ribosome assembly, ribosome maturation, pre-mRNA splicing, and mRNA degradation [ 7 , 35 ]. According to results of EdU and CCK8 assays, knockdown of DDX56 suppressed GC cell proliferation, while overexpression of DDX56 promoted proliferation.…”

Section: Discussionmentioning

confidence: 99%

DEAD-box helicase 56 functions as an oncogene promote cell proliferation and invasion in gastric cancer via the FOXO1/p21 Cip1/c-Myc signaling pathway

Wang

et al. 2022

Bioengineered

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DEAD-box helicase (DDX) family exerts a critical effect on cancer initiation and progression through alternative splicing, transcription and ribosome biogenesis. Increasing evidence has demonstrated that DEAD-box helicase 56 (DDX56) is over-expressed in several cancers, which plays an oncogenic role. Till the present, the impact of DDX56 on gastric cancer (GC) remains unclear. We conducted high-throughput sequencing (RNA-seq) to demonstrate aberrant DDX56 levels within 10 GC and matched non-carcinoma tissue samples. DDX56 levels were detected through qRT‐PCR, western blotting (WB) and immunochemical staining in GC patients. We conducted gain- and loss-of-function studies to examine DDX56’s biological role in GC development. In vitro, we carried out 5‑Ethynyl‑2‑deoxyuridine (EdU), scratch, Transwell, and flow cytometry (FCM) assays for detecting GC cell growth, invasion, migration and apoptosis. Additionally, gene set enrichment analysis (GSEA), WB assay, and Encyclopedia of RNA Interactomes (ENCORI) were carried out for analyzing DDX56-regulated downstream genes and signaling pathways. In vivo, tumor xenograft experiment was performed for investigating how DDX56 affected GC development within BALB/c nude mice. Functionally, DDX56 knockdown arrested cell cycle at G1 phase, invasion and migration of AGS and MKN28 cells, and enhanced their apoptosis. Ectopic DDX56 expression enhanced the cell growth, migration and invasion, and inhibited apoptosis. Knockdown of DDX56 suppressed GC growth in the tumor models of BALB/c nude mice. Mechanistically, DDX56 post-transcriptionally suppressed FOXO1/p21 Cip1 protein expression, which could activate its downstream cyclin E1/CDK2/c-Myc signaling pathways. This sheds lights on the GC pathogenic mechanism and offers a potential anti-cancer therapeutic target.

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“…DDX56 is a nucleolar protein and participates in ribosome biogenesis and ribosome assembly (68,69). Furthermore, DDX56 is involved in transcription (70), pre-mRNA splicing (71), as well as miRNA-mediated post-transcriptional regulation by degradation of primary miRNA (72).…”

Section: Ddx56mentioning

confidence: 99%

Role and therapeutic potential of DEAD-box RNA helicase family in colorectal cancer

Zheng,

Chen,

Ling

et al. 2023

Front. Oncol.

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Colorectal cancer (CRC) is the third most commonly diagnosed and the second cancer-related death worldwide, leading to more than 0.9 million deaths every year. Unfortunately, this disease is changing rapidly to a younger age, and in a more advanced stage when diagnosed. The DEAD-box RNA helicase proteins are the largest family of RNA helicases so far. They regulate almost every aspect of RNA physiological processes, including RNA transcription, editing, splicing and transport. Aberrant expression and critical roles of the DEAD-box RNA helicase proteins have been found in CRC. In this review, we first summarize the protein structure, cellular distribution, and diverse biological functions of DEAD-box RNA helicases. Then, we discuss the distinct roles of DEAD-box RNA helicase family in CRC and describe the cellular mechanism of actions based on recent studies, with an aim to provide future strategies for the treatment of CRC.

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Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex

Cited by 6 publications

References 48 publications

DEAD-Box RNA Helicases in Cell Cycle Control and Clinical Therapy

DEAD-Box RNA Helicases in Cell Cycle Control and Clinical Therapy

DEAD-box helicase 56 functions as an oncogene promote cell proliferation and invasion in gastric cancer via the FOXO1/p21 Cip1/c-Myc signaling pathway

Role and therapeutic potential of DEAD-box RNA helicase family in colorectal cancer

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