ddPCR provides a sensitive test compared with GeneXpert MTB/RIF and mNGS for suspected Mycobacterium tuberculosis infection

Zhang, Dan; Yu, Fei; Han, Dongsheng; Chen, Weizhen; Yuan, Lingjun; Xie, Mengxiao; Zheng, Jieyuan; Wang, Jingchao; Lou, Bin; Zheng, Shufa; Chen, Yu

doi:10.3389/fcimb.2023.1216339

Cited by 3 publications

(1 citation statement)

References 24 publications

(34 reference statements)

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“…However, a recent study on different clinical sample types, using dPCR, next-generation sequencing (NGS) and conventional commercial qPCR simultaneously, showed that the most sensitive method was dPCR (99%), over NGS (86%) and conventional qPCR (64%) [38].…”

Section: Bacteriology Applicationsmentioning

confidence: 99%

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Sancha Dominguez,

Cotos Suárez,

Sánchez Ledesma

et al. 2024

Diagnostics

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Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both in terms of sensitivity and specificity and in terms of the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation PCR, is based on a series of technical modifications that result in more sensitive techniques, more resistant to the action of inhibitors and capable of direct quantification without the need for standard curves. This review presents the main applications that have been developed for the diagnosis of viral, bacterial, and parasitic infections and the potential prospects for the clinical use of this technology.

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Section: Bacteriology Applicationsmentioning

confidence: 99%

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Sancha Dominguez,

Cotos Suárez,

Sánchez Ledesma

et al. 2024

Diagnostics

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Intensive reprocessing of reusable bronchoscopes can reduce the false positive rate of Xpert MTB/RIF caused by nucleic acid residue

Jin,

Hu,

et al. 2024

Journal of Clinical Tuberculosis and Other Mycobacterial Diseas

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Antimicrobial strategies of Lower Respiratory Tract Infection in Immunocompromised Patients based on Metagenomic Next-Generation Sequencing：a retrospective study

Meng,

Liu,

et al. 2024

Preprint

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Background Immunocompromised patients with Lower Respiratory Tract Infection(LRTI) frequently encounter a diverse range of pathogenic infections, characterized by rapid disease progression and significant mortality rates due to reckless or excessive utilization of antibiotics. Therefore, it is crucial to promptly and accurately identify the causative microorganisms for pathogen diagnosis and clinical decision-making. The objective of this study is to evaluate the clinical applicability of metagenomic next-generation sequencing (mNGS) in the diagnosis and management of LRTI, as well as its impact on empirical antibacterial therapy for patients with varying immune statuses. Methods We conducted a comparative analysis of positivity rate, detection accuracy, pathogen spectrum, duration of treatment (DOT), and antibiotic management in a cohort of 283 patients diagnosed with lower respiratory tract infections. Results The positive detection rate was higher in mNGS compared to conventional culture in both immunocompetent group (89.92% vs. 28.57%, P < 0.001) and immunocompromised group (84.44% vs. 33.33%, P < 0.001). The antibiotic escalation in the immunocompromised group was more frequent than that in the immunocompetent group (49.00% vs. 31.00%, P = 0.018), but no difference was observed for antibiotic de-escalation (20.00% vs. 15.00%, P = 0.458). Conclusions The application of mNGS can significantly enhance the pathogen detection rate and optimize antimicrobial drug management in immunocompromised patients with LRTI.

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ddPCR provides a sensitive test compared with GeneXpert MTB/RIF and mNGS for suspected Mycobacterium tuberculosis infection

Cited by 3 publications

References 24 publications

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis

Intensive reprocessing of reusable bronchoscopes can reduce the false positive rate of Xpert MTB/RIF caused by nucleic acid residue

Antimicrobial strategies of Lower Respiratory Tract Infection in Immunocompromised Patients based on Metagenomic Next-Generation Sequencing：a retrospective study

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