DC-SCRIPT deficiency delays mouse mammary gland development and branching morphogenesis

Tang, Chunling; Bijgaart, Renske J.E. van den; Looman, Maaike W. G.; Tel-Karthaus, Nina; Graaf, Annemarie M.A. de; Gilfillan, Susan; Colonna, Marco; Ansems, Marleen; Adema, Gosse J.

doi:10.1016/j.ydbio.2019.06.023

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…DC-SCRIPT expression has also been demonstrated in nonhematopoietic tissue (31,32). Thus, to confirm that the reduction in cDC1s that we observed with DC-SCRIPT loss was cell intrinsic, we generated mixed BM chimeras, whereby lethally irradiated CD45.1 + CD45.2 + hosts were reconstituted with a 1:1 BM mix of either WT CD45.1 + : Zfp366 −/− CD45.2 + or WT CD45.1 + :WT CD45.2 + .…”

Section: Cell-intrinsic Requirement For Dc-script In Cdc1mentioning

confidence: 99%

Type 1 conventional dendritic cell fate and function are controlled by DC-SCRIPT

et al. 2021

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The functional diversification of dendritic cells (DCs) is a key step in establishing protective immune responses. Despite the importance of DC lineage diversity, its genetic basis is not fully understood. The transcription factor DC-SCRIPT is expressed in conventional DCs (cDCs) and their committed bone marrow progenitors but not in plasmacytoid DCs (pDCs). We show that mice lacking DC-SCRIPT displayed substantially impaired development of IRF8 (interferon regulatory factor 8)–dependent cDC1, whereas cDC2 numbers increased marginally. The residual DC-SCRIPT–deficient cDC1s had impaired capacity to capture and present cell-associated antigens and to secrete IL-12p40, two functional hallmarks of this population. Genome-wide mapping of DC-SCRIPT binding and gene expression analyses revealed a key role for DC-SCRIPT in maintaining cDC1 identity via the direct regulation of cDC1 signature genes, including Irf8. Our study reveals DC-SCRIPT to be a critical component of the gene regulatory program shaping the functional attributes of cDC1s.

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Section: Cell-intrinsic Requirement For Dc-script In Cdc1mentioning

confidence: 99%