Dayak Onions ( Eleutherine bulbosa (Mill.) Urb.) Bulbs Extracts Reduces Blood Pressure of Hypoestrogen Model Rats by Controlling Lipids Profile

Bahtiar, Anton; Chumala, Delvika Yessi

doi:10.4067/s0717-95022018000301016

Cited by 2 publications

(1 citation statement)

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“…To date, the analysis of compounds in E. americana has been carried out using the thin layer chromatography method. The method was used to observe the antihypertensive activity and lipid profile in hypoestrogenic mice [7]. In addition, anthraquinone analysis is also carried out using Gas Chromatography-Mass Spectrometry (GC-MS) and High Performance Liquid Chromatography (HPLC) to determine the analyte as an active compound or contaminant [8][9][10].…”

Section: ■ Introductionmentioning

confidence: 99%

Simple Method of 9,10-Anthraquinone Assay in <i>Eleutherine americana</i> (Aubl.) Merr. ex K. Heyne using High-Performance Liquid Chromatography

et al. 2021

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Eleutherine americana (E. americana) is a medicinal plant commonly found on the island of Borneo, Indonesia. This plant is known to have several biological activities. However, anthraquinone residues are generally present as contaminants. This study was aimed to develop a method of determining the levels of 9,10-anthraquinone in plant extracts and fractions using High Performance Liquid Chromatography (HPLC). The research aims to optimize the mobile phase, the system suitability test, and the system validation. The optimal mobile phase was acetonitrile:distilled water 1:1 v/v with a flow rate of 1.25 mL/min. The validation result shows that the linearity was obtained with a correlation coefficient (r) of 0.9995 and an r2 coefficient of 0.9991. The estimated limits for detection and quantification values were 0.178 and 0.594 µg/mL, respectively. In the intraday and inter-day accuracy test, the coefficient of variance for reference was 0.627 and 0.774, while the results for the sample were 2.966 and 2.658. The percentage recovery rate for reference was between 98.976–101.452%, and for the sample, the result was 89.191–94.667%. The average 9,10-anthraquinone content in the acetate fraction of E. americana plant was 9.799 µg/g ± 5.243.

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Section: ■ Introductionmentioning

confidence: 99%