Day of cycle affects changes in equine intrauterine pressure in response to teasing

Stecco, Romana; Paccamonti, D.; Gutjahr, S.; Pinto, Carlos; Eilts, B.E.

doi:10.1016/s0093-691x(03)00076-1

Cited by 10 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study on mares estrus cycles without inseminations Campbell and England (2002) recognized that uterine contractions were lowest on the day of ovulation and that no significant differences were present between the other days of the cycle. UCA associated with teasing was similar during the time period between two days before ovulation and the day of ovulation, while two days after ovulation teasing did not stimulate UCA anymore (Stecco et al 2003). In the present study mares with a corpus hemorrhagicum at the time of the second insemination must have ovulated within the last 24 h, because at the time of first insemination a dominant follicle was still present in all mares.…”

Section: Discussionsupporting

confidence: 48%

Effect of oxytocin and seminal plasma treatment on uterine contractile activity and pregnancy rates in subfertile mares

Pansegrau¹,

Meinecke-Tillmann²,

Swagemakers³

et al. 2008

PHK

View full text Add to dashboard Cite

The aim of the study was to find out how reproductive disorders in problem mares, with a disposition to persistent mating induced endometritis, are related to dysfunctional uterine contractile activity (UCA). Additionally, seminal plasma, given into the uterus after insemination, and oxytocin administered immediately after insemination, were tested on their stimulating effects on uterine contractions and influence on pregnancy rates. Also the influence of UCA on uterine bacteriological and cytological findings before and after insemination was examined. The subfertile mares were inseminated into the uterine body 24 h and 48 h after hCG-administration and were treated immediately after AI with oxytocin (Group 1, n = 27 cycles) or additional seminal plasma (Group 2, n = 25 cycles) or were left untreated (Group 3, n = 30 cycles) after AI. Group 4 (healthy mares with foal, n = 12 cycles) was inseminated twice without further treatment and served as control. UCA was evaluated by transrectal ultrasonography 16 to 18 days after last estrus, before and after the first and second AI and 48 h after the second AI. Contractions were documented in a score-level-system (0-IV). Bacteriological and cytological samples were taken before the first and 48 h after the second AI. A uterine biopsy was analysed in interestrus before insemination. Significant differences in the UCA of the total group of mares were obtained between the different times of evaluation. Comparing the 4 treatment groups, the fertile control group showed in general stronger uterine contractions than the other groups. UCA between the fertile control and the oxytocin treated mares on the one side and the seminal plasma and untreated mares on the other side differed significantly. A positive correlation was shown between UCA and pregnancy results. The pregnancy rates differed significantly between the fertile control group (83 %) and the 3 experimental groups (40 %). There was no correlation between UCA and the results of the bacteriological and cytological samples of the endometrium. But there was a significant relationship between the results of the cytological sample obtained 48 h after the second insemination and the pregnancy results. Because of the positive correlation between UCA and pregnancy rate, poor uterine contractions are seen as one of the major causes of subfertility in mares. The present study demonstrated that uterine contractions are stimulated by artificial insemination and that consecutive inseminations even intensified uterine contractions. Barren mares showed in general a lower UCA than fertile mares did. To obtain good pregnancy results, also negative cytological swabs 48 h after insemination were important, since mares with positive results at this time had a minor chance of becoming pregnant. Based on these results optimal insemination frequency and way and time of uterine sampling should be discussed in the management of broodmares.

show abstract

Section: Discussionsupporting

confidence: 48%

Effect of oxytocin and seminal plasma treatment on uterine contractile activity and pregnancy rates in subfertile mares

Pansegrau¹,

Meinecke-Tillmann²,

Swagemakers³

et al. 2008

PHK

View full text Add to dashboard Cite

show abstract

“…In a previous ultrasonographic study, the number of contractions was lowest on the day of ovulation, but no significant differences were found among the other days of the cycle (Campbell and England, 2002). Stecco et al. (2003) found no difference in uterine contractions associated with teasing between the day of ovulation and 2 days before ovulation, but 2 days after ovulation in the presence of increasing progesterone levels teasing no longer provoked uterine contractions.…”

Section: Discussionmentioning

confidence: 64%

Effect of Insemination Volume on Uterine Contractions and Inflammatory Response and on Elimination of Semen in the Mare Uterus – Scintigraphic and Ultrasonographic Studies

Sinnemaa

Järvimaa

Lehmonen

et al. 2005

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

The effect of artificial insemination (AI) volume on uterine contractility and inflammation and on elimination of semen in the reproductive tract of mares was examined for 4 h after AI using two methods, scintigraphy and ultrasonography. The same doses were used in both methods: 2 and 100 ml of skim milk-extended frozen semen. In the scintigraphic study, the number of reproductively normal mares was four per group and in the ultrasonographic study five per group. For scintigraphy, the semen was radiolabelled with technetium-99m. The static scintigrams were acquired immediately before and 30, 60, 120, 180 and 240 min after AI. The activities in the vagina and uterus were calculated and the values for sperm that had been discharged from the mare were obtained by subtracting the counts for the uterus and vagina from the total radioactivity. The dynamic scintigrams were taken continuously for the first 30 min after AI and in 5-min periods immediately after having acquired the static scintigrams. The uterine contractions were counted. In the ultrasonographic study, the mares were scanned before AI and at 5, 10, 15, 20, 25, 30, 60, 120, 150, 180, and 240 min after AI, for at least 1 min each time. The examinations were videotaped and contractions counted per minute. More contractions were observed with the ultrasonographic method than with the scintigraphic method. No difference was present in the number of contractions between the groups, except in the ultrasonographic study at 4 h, when the mares inseminated with 100 ml showed more contractions than did the mares inseminated with 2 ml. The intraluminal fluid was sampled with a tampon and by uterine lavage 4 h after AI in the ultrasonographic study. The numbers of polymorphonuclear leukocytes and spermatozoa were counted, but the differences between the groups were not significant. Under our experimental conditions and with the number of mares examined, the volume of the AI dose had an insignificant effect on contractility - with the exception at 4 h - and inflammatory reaction and on semen elimination in the uterus.

show abstract

“…In the present experiment, it was possible to use telemetric implants for registering the EMG of the uterus and oviduct because the bipolar electrodes that were used were custom made. The commonly used unipolar electrodes are not designed to fit the anatomical shape of the oviducts and uteri of different species (Gajewski et al 1991;Stecco et al 2003;Cavaco-Gonçalves et al 2006 animals. Reproducibility of conditions is one of the key elements of the research methodology in long-term tests on animals.…”

Section: Discussionmentioning

confidence: 99%

“…Results of recordings of the contractility of the oviduct and uterus obtained using in vitro research techniques are debatable, mainly because they do not take into consideration basic variables, such as the hormonal effects in the blood and the impact of the autonomic nervous system (Gajewski & Faundez, ; Stecco et al . ; Garfield et al . ).…”

Section: Introductionmentioning

confidence: 99%

“…Results obtained during experiments on this species are treated as referential. Results of recordings of the contractility of the oviduct and uterus obtained using in vitro research techniques are debatable, mainly because they do not take into consideration basic variables, such as the hormonal effects in the blood and the impact of the autonomic nervous system (Gajewski & Faundez, 1992;Stecco et al 2003;Garfield et al 2005). The myoelectrical activity data of the uterus is fragmentary, limited by technical problems (recordings lasting only up to a few hours do not cover the whole oestrous cycle) and complicated surgical operations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characteristics of bioelectrical activity of oviducts and uterus during early pregnancy in sows recorded by telemetry method

Pawliński

Domino

Zabielski

et al. 2017

Experimental Physiology

View full text Add to dashboard Cite

What is the central question of this study? The aim of present study was to record and analyse the myoelectrical activity in the female pig reproductive tract (uterus and oviduct) during early pregnancy. What is the main finding and its importance? Understanding the contractile activity of the uterus and oviducts is indispensable for understanding the physiological mechanisms as well as all irregularities associated with the period of conception and early pregnancy. The aim of the present study was to record the myoelectrical activity of the reproductive tract in sows during the oestrous phase and early pregnancy via a telemetry recording system. In a total of eight non-pregnant pigs, the bioelectrical activity was recorded through three silicone electrodes sutured on the oviduct (isthmus and ampulla) and the uterine horn. Blood samples were collected to monitor the concentrations of progesterone (P4) and luteinizing hormone (LH). The oestrous cycle was synchronized with equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG), and the animals were subjected to artificial insemination. Analysis of the EMG activity of the oviduct and uterus in the oestrous phase and in early stages of pregnancy suggests explicitly that telemetry could enable in vivo assessment of myoelectrical activity of parts of the reproductive system in sows. Off-line analysis of the duration of EMG activity bursts in the uterus, isthmus and ampulla were significantly higher during early pregnancy (phases II and III) than in the oestrous phase. The EMG signals demonstrated low mean amplitudes of activity in the oviduct and uterus during early pregnancy (phases I-III). Significant differences between the root mean square signals were observed in the isthmus and ampulla both during oestrus and in early pregnancy (phase I; P < 0.01). During the oestrous phase, the P4 concentration was estimated at <1 ng ml , whereas the LH concentration was >4 ng ml . In contrast, during early pregnancy, the P4 and LH concentrations were estimated at >4 and <1 ng ml , respectively.

show abstract

Day of cycle affects changes in equine intrauterine pressure in response to teasing

Cited by 10 publications

References 16 publications

Effect of oxytocin and seminal plasma treatment on uterine contractile activity and pregnancy rates in subfertile mares

Effect of oxytocin and seminal plasma treatment on uterine contractile activity and pregnancy rates in subfertile mares

Effect of Insemination Volume on Uterine Contractions and Inflammatory Response and on Elimination of Semen in the Mare Uterus – Scintigraphic and Ultrasonographic Studies

Characteristics of bioelectrical activity of oviducts and uterus during early pregnancy in sows recorded by telemetry method

Contact Info

Product

Resources

About