Day/night and circadian rhythm control of con gene expression in Neurospora.

Lauter, Frank-Roman; Yanofsky, Charles

doi:10.1073/pnas.90.17.8249

Cited by 79 publications

(73 citation statements)

References 31 publications

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“…However, the amount of con-10 mRNA that accumulated in light-exposed mycelia was increased in strains with mutations in acon-3, fl, and fld, which suggested that the corresponding gene products should repress con-10 photoactivation ( Figure 4A). The increase in the accumulation of con-10 mRNA after light exposure in these developmental mutants had been reported after RNA hybridization experiments (Lauter and Yanofsky 1993), further supporting our results.…”

Section: Er53supporting

confidence: 81%

“…In addition, we assayed light-dependent fl mRNA accumulation in different fl mutants. As a control, we assayed the light-dependent accumulation of con-10 mRNA since light regulation for this gene has been observed in several developmental mutants (Lauter and Yanofsky 1993). We observed that fl was activated by light in all the developmental mutants that we tested, with the exception of fld ( Figure 4A).…”

Section: Er53mentioning

confidence: 95%

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Regulation by Blue Light of the fluffy Gene Encoding a Major Regulator of Conidiation in Neurospora crassa

Olmedo¹,

Ruger-Herreros

Corrochano

2010

Genetics

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The development of asexual spores, that is, the process of conidiation, in the fungus Neurospora crassa is increased by light. The fluffy (fl) gene, encoding a major regulator of conidiation, is activated by light. We describe here a detailed characterization of the regulation by blue light of fl in vegetative hyphae. This induction requires the white collar complex (WCC) while the FLD protein acts as a dark repressor of fl transcription. We show that the WCC directly regulates fl transcription in response to blue light after transiently binding the promoter. We propose that fl is repressed by FLD in vegetative mycelia and that the repression is lost after light exposure and WCC activation. The increase in fl mRNA in vegetative mycelia after light exposure, and the corresponding increase in the amount of the regulatory FL protein, should promote the activation of the conidiation pathway. The activation by light of fl provides a simple mechanism for the activation of conidiation by blue light in Neurospora that may be at work in other fungi.

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Section: Er53supporting

confidence: 81%

Section: Er53mentioning

confidence: 95%

Regulation by Blue Light of the fluffy Gene Encoding a Major Regulator of Conidiation in Neurospora crassa

Olmedo¹,

Ruger-Herreros

Corrochano

2010

Genetics

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“…Photoadaptation has been described for light-regulated genes in N. crassa (Lauter and Yanofsky 1993;Arpaia et al 1999;Schwerdtfeger and Linden 2003) and P. blakesleeanus (Rodriguez-Romero and Corrochano 2006). Photoadaptation in N. crassa requires the product of the vvd gene (Heintzen et al 2001;Schwerdtfeger and Linden 2003;Zoltowski et al 2007), such that the activity of the white-collar complex (WCC) is controlled by the VVD protein through the direct interaction of VVD with the components of the WCC (Chen et al 2010a;Hunt et al 2010;Malzahn et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Regulation of Conidiation by Light in Aspergillus nidulans

et al. 2011

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Light regulates several aspects of the biology of many organisms, including the balance between asexual and sexual development in some fungi. To understand how light regulates fungal development at the molecular level we have used Aspergillus nidulans as a model. We have performed a genome-wide expression analysis that has allowed us to identify .400 genes upregulated and .100 genes downregulated by light in developmentally competent mycelium. Among the upregulated genes were genes required for the regulation of asexual development, one of the major biological responses to light in A. nidulans, which is a pathway controlled by the master regulatory gene brlA. The expression of brlA, like conidiation, is induced by light. A detailed analysis of brlA light regulation revealed increased expression after short exposures with a maximum after 60 min of light followed by photoadaptation with longer light exposures. In addition to brlA, genes flbA-C and fluG are also light regulated, and flbA-C are required for the correct lightdependent regulation of the upstream regulator fluG. We have found that light induction of brlA required the photoreceptor complex composed of a phytochrome FphA, and the white-collar homologs LreA and LreB, and the fluffy genes flbA-C. We propose that the activation of regulatory genes by light is the key event in the activation of asexual development by light in A. nidulans.

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“…The con-6 and con-10 proteins are present in a second type of asexual spore, the microconidium (10,13), and the con-10 protein is present in sexual spores (10, 13). Expression of con-6 and con-10 can also be induced in vegetative mycelial cultures by light exposure or by imposing conditions that induce circadian rhythm (10,(13)(14)(15). We describe here the selection and partial characterization of mutants that express con-6 and con-10 aberrantly during vegetative mycelial growth.…”

Section: Introductionmentioning

confidence: 99%

Mutants of Neurospora crassa that alter gene expression and conidia development.

Madi

Ebbole²,

White³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Several genes have been identied that are highly expressed during conidiation. Inactivation ofthese genes has no observable phenotypic effect. Transcripts of two such genes, con-6 and con-10, are norally absent from vegetative mycella. To identify regulatory genes that affect con-6 and/or con-10 expression, strains were prepared in which the regulatory regions for these genes were fused to a gene conferrin hygromycin resistance. Mutants were then selected that were resistant to the drug during mycelial growth. Mutations in several of the isolates had trans effects; they activated transcription of the corresponding intact gene and, in most oates, one or more ofthe other con genes. Most Analyses of the protein and mRNA species present in mycelia, aerial hyphae, and conidia have revealed significant differences in gene expression (3)(4)(5). A differential screening procedure was used to clone genes that are preferentially expressed during conidiation (4). Four of these genes, con-6, con-8, con-10, and con-13, have been sequenced, and their expression has been examined (6-10). A fifth conidiationspecific gene, eas, also has been characterized (11, 12); eas encodes the rodlet protein that coats the surface ofthe mature conidium.con-6 and con-10 encode 93-and 86-residue polypeptides, respectively; the functions of these polypeptides are unknown. con-6 and con-10 transcripts and proteins are normally undetectable in vegetative mycelium but appear at high levels during conidiation and in mature conidia; these transcripts and proteins disappear a few hours after conidia germinate (6,13). The expression pattern of these genes closely parallels that of eas (11, 12). The con-6 and con-10 proteins are present in a second type of asexual spore, the microconidium (10,13), and the con-10 protein is present in sexual spores (10, 13). Expression of con-6 and con-10 can also be induced in vegetative mycelial cultures by light exposure or by imposing conditions that induce circadian rhythm (10,(13)(14)(15). We describe here the selection and partial characterization of mutants that express con-6 and con-10 aberrantly during vegetative mycelial growth. Some of these mutants were found to be blocked in conidiation, whereas others exhibited different developmental abnormalities. MATERIALS AND METHODSStrains and Pids. Strain CH10 was constructed by homologous integration, using pCH10, a plasmid containing a con-10 translational fusion to the hygromycin phosphotransferase gene (hph) (16). Integration was at the his-3 locus of Fungal Genetics Stock Center (FGSC) strain 462: his-3 A. The integrated plasmid consisted of pBluescript KS+ (Promega) vector containing con-10 DNA from positions 1139 to 1935 (8); this segment contains the first 40 codons of con-10. The con-10 DNA is fused in-frame to the second codon of hph followed by the Aspergillus nidulans trpC transcription termination region from plasmid pDH25 (16). The plasmid also contains a 5' truncated copy of the his-3 gene that allows reconstitution of an intact copy of the ...

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Day/night and circadian rhythm control of con gene expression in Neurospora.

Cited by 79 publications

References 31 publications

Regulation by Blue Light of the fluffy Gene Encoding a Major Regulator of Conidiation in Neurospora crassa

Regulation by Blue Light of the fluffy Gene Encoding a Major Regulator of Conidiation in Neurospora crassa

Regulation of Conidiation by Light in Aspergillus nidulans

Mutants of Neurospora crassa that alter gene expression and conidia development.

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