Day-3 embryo metabolomics in the spent culture media is altered in obese women undergoing in vitro fertilization

Bellver, José; Santos, María José de los; Alamá, Pilar; Castelló, D; Privitera, Laura; Galliano, Daniela; Labarta, Elena; Vidal, C.; Pellicer, António; Domı́nguez, Francisco

doi:10.1016/j.fertnstert.2015.03.015

Cited by 35 publications

(13 citation statements)

References 41 publications

(50 reference statements)

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“…Compared with normal-BMI women, severe obesity (BMI R35 kg/m 2 ) is associated with a greater prevalence of spindle anomalies and nonaligned chromosomes in failed fertilized oocytes (101). In addition, the metabolomic profile of spent culture media of day-3 embryos of obese women differs from that of normal-weight women, with significant reductions in the concentration of saturated fatty acids (102).…”

Section: Embryo Factorsmentioning

confidence: 99%

Adverse effects of female obesity and interaction with race on reproductive potential

Luke

2017

Fertility and Sterility

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Across the reproductive spectrum, obesity is associated with greater risks for adverse health outcomes, including higher rates of infertility, subfertility, early pregnancy loss, fetal deaths and stillbirths, congenital anomalies, and pregnancy complications. The excess reproductive morbidity associated with obesity may increase with longer duration, making the current trends among children and young adults particularly critical in terms of their future reproductive potential. Obese women have a lower chance of pregnancy following in vitro fertilization (IVF), require higher dosages of gonadotropins, and have reduced rates of implantation, clinical intrauterine gestation, and live birth rates and increased rates of pregnancy loss, as well as greater risks for prematurity and preeclampsia even when stratified by plurality. Racial and ethnic differences by overweight and obesity in IVF outcomes have been reported. Compared with normal-weight women, failure to achieve a clinical intrauterine gestation is significantly more likely among obese women overall, normal-weight and obese Asian women, normal-weight Hispanic women, and overweight and obese Black women. Among women who do conceive, compared with normal-weight women, failure to achieve a live birth is significantly more likely among overweight and obese women overall, and among overweight and obese Asian women, overweight and obese Hispanic women, and normal-weight and obese Black women. Although weight loss should theoretically be the first line of therapy for obese women, other lifestyle factors, such as regular physical exercise, elimination of tobacco use and alcohol consumption, and stress management, may be of more immediate benefit in achieving conception.

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Section: Embryo Factorsmentioning

confidence: 99%

Adverse effects of female obesity and interaction with race on reproductive potential

Luke

2017

Fertility and Sterility

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“…(5) A step forward was made in recent years by metabolomics technology, aiming to identify metabolites that represent chemical fingerprints of various metabolic pathways relevant for the pathogenesis of PCOS. Metabolomic profiling in PCOS has made its first steps in the early 2012, with few studies that focused on characterization of the metabolomic signature of lipid metabo-lism, hormone biosynthesis, medical therapies, and assisted reproduction outcomes (6)(7)(8)(9)(10). Objectives: By performing a systematic search of the literature, we aimed to summarize metabolomic pathways and biomarkers associated with obesity in women with PCOS.…”

Section: Rationale and Backgroundmentioning

confidence: 99%

Metabolomic biomarkers of polycystic ovary syndrome related-obesity: a review of the literature

Vonica

Fărcăş

Román

et al. 2020

Revista Romana De Medicina De Laborator

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Background and objectives: Polycystic ovary syndrome (PCOS) displays a phenotype-dependent cardio-metabolic risk. By performing a systematic search of the literature, we aimed to summarize metabolomic signatures associated with obesity in PCOS women.Data sources and study eligibility criteria: We conducted a comprehensive search including: Embase, PubMed, and Web of Science until 31st of May 2019. We used the terms: metabolomics and polycystic ovary syndrome. We excluded the following papers: animal studies, studies that included only lean PCOS women, reviews, meta-analyses, results of interventional studies, those that did not apply metabolomic techniques.Results: The lipid signature in obese women with PCOS showed increased levels of free fatty acids (carnitine, adipic acid, linoleic acid, oleic acid) and lower levels of lysophosphatidylcholines and glycerolphosphocholine compared with non-obese PCOS women. Regarding carbohydrate metabolism, a decrease in citric and lactic acid levels characterized obese PCOS women. Decreased lactic acid in obese PCOS women suggests augmented insulin stimulated glucose muscle use in lean, but not in obese women. Considering amino acid metabolomic markers, valine, glycine, serine, threonine, isoleucine and lysine were higher in obese PCOS women. Patients with visceral obesity presented a diminished uptake of essential amino acids, BCAA, leucine and serine in the skeletal muscle. α-ketoglutarate was significantly higher in obese women with PCOS in comparison with lean women with PCOS, distinguishing these 2 subgroups of PCOS with high ‘predictive accuracy’.Limitations: Overall, a small number of studies have focused on the impact of obesity on the metabolic fingerprints of PCOS women. There is need for properly controlled, high-quality studies.Conclusions: There is compelling evidence of significant alterations in carbohydrate, lipid, and amino acid metabolism in women with PCOS and obesity. Metabolomics may identify new metabolic pathways involved in PCOS and improve our understanding of the complex relation between PCOS and obesity in order to personalize PCOS therapy.

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“…In our previous study [ 29 ] we used a ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS) untargeted metabolomic analysis to determine the differential global metabolic status of embryos coming from obese and normoweight IVF patients using spent media culture. Indeed, using this kind of approach, we obtained clear differences in the metabolism between these embryos.…”

Section: Introductionmentioning

confidence: 99%

The Metabolomic Profile of Spent Culture Media from Day-3 Human Embryos Cultured under Low Oxygen Tension

et al. 2015

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Despite efforts made to improve the in vitro embryo culture conditions used during assisted reproduction procedures, human embryos must adapt to different in vitro oxygen concentrations and the new metabolic milieu provided by the diverse culture media used for such protocols. It has been shown that the embryo culture environment can affect not only cellular metabolism, but also gene expression in different species of mammalian embryos. Therefore we wanted to compare the metabolic footprint left by human cleavage-stage embryos under two types of oxygen atmospheric culture conditions (6% and 20% O2). The spent culture media from 39 transferred and implanted embryos from a total of 22 patients undergoing egg donation treatment was analyzed; 23 embryos came from 13 patients in the 6% oxygen concentration group, and 16 embryos from 9 patients were used in the 20% oxygen concentration group. The multivariate statistics we used in our analysis showed that human cleavage-stage embryos grown under both types of oxygen concentration left a similar metabolic fingerprint. We failed to observe any change in the net depletion or release of relevant analytes, such as glucose and especially fatty acids, by human cleavage-stage embryos under either type of culture condition. Therefore it seems that low oxygen tension during embryo culture does not alter the global metabolism of human cleavage-stage embryos.

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Day-3 embryo metabolomics in the spent culture media is altered in obese women undergoing in vitro fertilization

Cited by 35 publications

References 41 publications

Adverse effects of female obesity and interaction with race on reproductive potential

Adverse effects of female obesity and interaction with race on reproductive potential

Metabolomic biomarkers of polycystic ovary syndrome related-obesity: a review of the literature

The Metabolomic Profile of Spent Culture Media from Day-3 Human Embryos Cultured under Low Oxygen Tension

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