Dawn regulates guard cell proteins in Arabidopsis thaliana that function in ATP production from fatty acid beta-oxidation

Geilfus, Christoph‐Martin; Lan, Jue; Carpentier, Sébastien

doi:10.1007/s11103-018-0794-x

Cited by 12 publications

(18 citation statements)

References 76 publications

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“…Thus, some GCs specific channel proteins such as SLAC1 and KAT1 were not observed in our study. This is in an agreement with previous proteomics reports conducted in Arabidopsis [4,9].…”

Section: Comparison Of Ground State Mesophyll and Guard Cells Proteomesupporting

confidence: 94%

“…Thus, some GCs specific channel proteins such as SLAC1 and KAT1 were not observed in our study. This is in an agreement with previous proteomics reports conducted in Arabidopsis [4,9]. To further characterize the GC proteome, we used Mercator4 software in conjunction with MapMan pathway analysis for protein functional classification.…”

Section: Comparison Of Ground State Mesophyll and Guard Cells Proteomesupporting

confidence: 82%

“…In most proteomics studies, GCs are not analyzed separately but included in the bulk of the leaf samples, thus masking GC-specific mechanisms underlying stomatal operation under stress conditions. To date, a very limited number of studies have used proteomics to analyze GCs specifically [4,[8][9][10]29] and none have investigated the specific response of GCs to salt stress. In this study, the efficiency of the GC isolation method was validated with a combination of techniques, including microscopic observation, viability tests, and at the proteome level.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to GCs, proteins involved in photosynthesis and starch synthesis were enriched in mesophyll cells. Proteomics has also been used to analyze the response of GCs to environmental cues, including light, CO 2 , and ABA [9,10]. For example, proteins and metabolites associated with fatty acid metabolism, redox regulation, and starch /sucrose metabolism were identified in Brassica napus GCs in response to low CO 2 [9].…”

Section: Introductionmentioning

confidence: 99%

“…Proteomics has also been used to analyze the response of GCs to environmental cues, including light, CO 2 , and ABA [9,10]. For example, proteins and metabolites associated with fatty acid metabolism, redox regulation, and starch /sucrose metabolism were identified in Brassica napus GCs in response to low CO 2 [9]. This finding shows that the knowledge obtained from proteomic experiments on GCs can boost our understanding of specific cellular events and key proteins involved in stress conditions and provide information about molecular networks underlying the stomatal functions in different conditions.…”

Section: Introductionmentioning

confidence: 99%

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Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Rasouli

Kiani‐Pouya

et al. 2020

IJMS

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Soil salinity is a major environmental constraint affecting crop growth and threatening global food security. Plants adapt to salinity by optimizing the performance of stomata. Stomata are formed by two guard cells (GCs) that are morphologically and functionally distinct from the other leaf cells. These microscopic sphincters inserted into the wax-covered epidermis of the shoot balance CO2 intake for photosynthetic carbon gain and concomitant water loss. In order to better understand the molecular mechanisms underlying stomatal function under saline conditions, we used proteomics approach to study isolated GCs from the salt-tolerant sugar beet species. Of the 2088 proteins identified in sugar beet GCs, 82 were differentially regulated by salt treatment. According to bioinformatics analysis (GO enrichment analysis and protein classification), these proteins were involved in lipid metabolism, cell wall modification, ATP biosynthesis, and signaling. Among the significant differentially abundant proteins, several proteins classified as “stress proteins” were upregulated, including non-specific lipid transfer protein, chaperone proteins, heat shock proteins, inorganic pyrophosphatase 2, responsible for energized vacuole membrane for ion transportation. Moreover, several antioxidant enzymes (peroxide, superoxidase dismutase) were highly upregulated. Furthermore, cell wall proteins detected in GCs provided some evidence that GC walls were more flexible in response to salt stress. Proteins such as L-ascorbate oxidase that were constitutively high under both control and high salinity conditions may contribute to the ability of sugar beet GCs to adapt to salinity by mitigating salinity-induced oxidative stress.

show abstract

“…Thus, some GCs specific channel proteins such as SLAC1 and KAT1 were not observed in our study. This is in an agreement with previous proteomics reports conducted in Arabidopsis [4,9].…”

Section: Comparison Of Ground State Mesophyll and Guard Cells Proteomesupporting

confidence: 94%

Section: Comparison Of Ground State Mesophyll and Guard Cells Proteomesupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Rasouli

Kiani‐Pouya

et al. 2020

IJMS

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Impact of nutritional imbalance on guard cell metabolism and stomata regulation under saline hyperosmotic conditions

Zörb

Franzisky

Lehr

et al. 2022

Stomata Regulation and Water Use Efficiency in Plants Under Saline Soil Conditions

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Application of omics technologies in single-type guard cell studies for understanding the mechanistic basis of plant adaptation to saline conditions

Rasouli

Kiani‐Pouya

Wang

et al. 2022

Stomata Regulation and Water Use Efficiency in Plants Under Saline Soil Conditions

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Dawn regulates guard cell proteins in Arabidopsis thaliana that function in ATP production from fatty acid beta-oxidation

Cited by 12 publications

References 76 publications

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Sugar Beet (Beta vulgaris) Guard Cells Responses to Salinity Stress: A Proteomic Analysis

Impact of nutritional imbalance on guard cell metabolism and stomata regulation under saline hyperosmotic conditions

Application of omics technologies in single-type guard cell studies for understanding the mechanistic basis of plant adaptation to saline conditions

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