Dauricine inhibits insulin-like growth factor-I-induced hypoxia inducible factor 1α protein accumulation and vascular endothelial growth factor expression in human breast cancer cells

Tang, Xudong; Zhou, Xin; Zhou, Keyuan

doi:10.1038/aps.2009.8

Cited by 41 publications

(30 citation statements)

References 27 publications

(37 reference statements)

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“…Recently, it has been shown that the expression of HIF-1α can be upregulated not only by hypoxia, but also by other nonhypoxic factors, such as the inactivation of tumor-suppressor genes (p53, pVHL, and PTEN [35,36,37]), activation of some viral oncoproteins (EBV and HPV-16 [38,39]), and challenge by IGF-I [40,41,42], etc. In the present study, the human NSCLC cell line A549 was used to investigate the effect of EGCG on the IGF-I-induced HIF-1α and VEGF expression and the proangiogenic activity of human lung cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin-3-Gallate Inhibits IGF-I-Stimulated Lung Cancer Angiogenesis through Downregulation of HIF-1α and VEGF Expression

Li¹,

Feng²,

Liu³

et al. 2013

Lifestyle Genomics

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Background/Aims: Numerous studies have shown that epigallocatechin-3-gallate (EGCG), a polyphenol component extracted from green tea, can inhibit the growth and induce apoptosis of various types of human tumor cells. In this study, we evaluated the inhibitory effects of EGCG on the proangiogenic capabilities of A549 cells. Methods: A549 cells starved in serum-free culture medium for 24 h were pretreated with EGCG at various concentrations (0, 10, 25, 50, and 100 μmol/l) for 1 h, followed by the addition of insulin-like growth factor-I (IGF-I) at the final concentration of 40 ng/ml and continued culturing for an additional 16 h. The in vitro angiogenesis analyzing test kit with ECMatrix™ gel was used to detect the formation of capillary tube-like structures. The mRNA expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) was determined by real-time PCR. The protein expression of HIF-1α and VEGF was detected by Western blotting and ELISA, respectively. Results: EGCG significantly inhibited the formation of capillary tube-like structures on the surface of ECMatrix induced by IGF-I both in vitro and in vivo and reduced the level of hemoglobin in Matrigel plugs. In addition, EGCG was shown to significantly inhibit the IGF-I-induced upregulation of HIF-1α protein expression. Meanwhile, EGCG at the concentration of 25 and 100 μmol/l exhibited obvious inhibitory effects on IGF-I-induced VEGF expression (p < 0.01). Conclusion: Our results suggest that EGCG has potent inhibitory effects on tumor angiogenesis induced by IGF-I in human non-small cell lung cancer cells, which may possibly contribute to the downregulation of HIF-1α and VEGF expression.

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Section: Discussionmentioning

confidence: 99%

Epigallocatechin-3-Gallate Inhibits IGF-I-Stimulated Lung Cancer Angiogenesis through Downregulation of HIF-1α and VEGF Expression

Li¹,

Feng²,

Liu³

et al. 2013

Lifestyle Genomics

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show abstract

“…It was proved to suppress proliferation and invasion and induce apoptosis of HCT116 and HCT8 human colon cancer cells by blocking the NF-κB signaling pathway (79). In the MCF-7 human breast cancer line and HUVECs human umbilical vein endothelial cells, dauricine plays a role in inhibiting angiogenesis of cancer cells by blocking the PI3K/Akt/mTOR pathway and HIF-1α pathway (80).…”

Section: Berbamine and Its Structural Analoguesmentioning

confidence: 99%

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

Xia

Chen

Zhang

et al. 2014

DD^|^T

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“…1 for their chemical structures). Dauricine, a calcium channel blocker, is the main toxin that makes M. dauricum poisonous [4], has been studied by many scientists for its high pharmacological activities [5][6][7]. However, little pharmacological research has been done on its structure analogues such as daurisolin, daurinoline and dauricicoline.…”

Section: Introductionmentioning

confidence: 99%

Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

Luo

Peng

et al. 2010

Journal of Chromatography B

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Dauricine inhibits insulin-like growth factor-I-induced hypoxia inducible factor 1α protein accumulation and vascular endothelial growth factor expression in human breast cancer cells

Cited by 41 publications

References 27 publications

Epigallocatechin-3-Gallate Inhibits IGF-I-Stimulated Lung Cancer Angiogenesis through Downregulation of HIF-1α and VEGF Expression

Epigallocatechin-3-Gallate Inhibits IGF-I-Stimulated Lung Cancer Angiogenesis through Downregulation of HIF-1α and VEGF Expression

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography

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