Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

Chen, Baoan; Lai, Binbin; Cheng, Jian; Xia, Guohua; Gao, Feng; Xu, Wenlin; Ding, Jia-Hua; Gao, Chong; Sun, Xinchen; Xu, Cuirong; Chen, Wenji; Chen, Ning-Na; Liu, Lijie; Li, Xiaomao; Wang, Xuemei

doi:10.2147/ijn.s7287

Cited by 30 publications

(23 citation statements)

References 44 publications

(46 reference statements)

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“…It has been previously reported that MNP-Fe 3 O 4 with anticancer drugs could effectively improve efficiency of chemotherapeutic agents which has a synergistic effect in multidrug resistance. 12,13 Our present study demonstrated that the unique MNPsFe 3 O 4 could increase the biological effects and decrease the side effects of ART. MTT results revealed that after being combined with MNPs-Fe 3 O 4 (100:1, M/M), ART showed an enhanced cytotoxic effect on K562 cells; the copolymer of 100 µM ART with 1 µM MNPs-Fe 3 O 4 had the most enhanced cytotoxicity among the five groups.…”

Section: Discussionsupporting

confidence: 53%

“…10,11 We have recently reported that MNPs-Fe 3 O 4 , as an anticancer drug deliverer, could enhance the sensitivity of anticancer drugs and reverse the drug resistance of tumor cells. 12,13 Apoptosis is the main mechanism of cell death, and is mediated by a cell-intrinsic suicide program, with the relative balance of pro-and anti-apoptotic signaling pathways determining the fate of the cell. There are two main pathways in mammals.…”

Section: Introductionmentioning

confidence: 99%

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Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

Wang

Han²,

Yang³

et al. 2011

IJN

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The present study evaluated whether the magnetic nanoparticles of Fe 3 O 4 (MNPs-Fe 3 O 4 ) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe 3 O 4 on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot. After being incubated with the copolymer of ART with MNPs-Fe 3 O 4 for 48 hours, the growth inhibition rate of K562 cells was significantly increased compared with that of K562 cells treated with ART alone ( P < 0.05), and the apoptosis rate of K562 cells was increased significantly compared with that of K562 cells treated with ART alone, suggesting that MNPs-Fe 3 O 4 can enhance the activity of ART. Interestingly, the copolymer-induced cell death was attenuated by caspase inhibitor Z-VAD-FMK. Our results also showed that treatment with the copolymer of MNPs-Fe 3 O 4 and ART increased the expression of bcl-2, bax, bcl-rambo, and caspase-3 proteins, and decreased the expression of survivin protein in K562 cells compared with ART treatment alone. These results suggest that MNPs-Fe 3 O 4 can enhance ART-induced apoptosis, which may be related to the upregulation of bcl-rambo and downregulation of survivin.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

Wang

Han²,

Yang³

et al. 2011

IJN

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“…Similar results are found for the percentage of apoptotic K562 cells induced by DNR-Sol and DNR-MNPs. In a previous study by our group, 33,34 daunorubicin-loaded magnetic nanoparticles of Fe 3 O 4 were more effective than daunorubicin alone in multidrug-resistant cells. Our present synthesis method is different to the previous one, which relied on mechanical absorption and polymerization, resulting in loose linkage of DNR and MNPs.…”

Section: Discussionmentioning

confidence: 82%

Synthesis and antitumor efficacy of daunorubicin-loaded magnetic nanoparticles

Wang¹,

Chen²,

Chen³

et al. 2011

IJN

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Background A promising approach to optimize the disposition of daunorubicin-loaded magnetic nanoparticles (DNR-MNPs) was developed to minimize serious side effects of systematic chemotherapy for cancer. Methods The physical properties of DNR-MNPs were investigated and their effect on leukemia cells in vitro was evaluated by a standard WST-1 cell proliferation assay. Furthermore, cell apoptosis and intracellular accumulation of DNR were determined by FACSCalibur flow cytometry. Results Our results showed that the majority of MNPs were spherical and their sizes were from 10 to 20 nm. The average hydrodynamic diameter of DNR-MNPs in water was 94 nm. The in vitro release data showed that the DNR-MNPs have excellent sustained release property. Proliferation of K562 cells was inhibited in a dose-dependent manner by DNR in solution (DNR-Sol) or by DNR-MNPs. The IC 50 for DNR-MNPs was slightly higher than that for DNR-Sol. DNR-MNPs also induced less apoptosis in K562 cells than did DNR-Sol. Detection of fluorescence intensity of intracellular DNR demonstrated that DNR-MNPs could be taken up by K562 cells and persistently released DNR in cells. Conclusion Our study suggests that optimized DNR-MNPs formulation possesses sustained drug-release and favorable antitumor properties, which may be used as a conventional dosage form for antitumor therapy.

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“…[26][27][28] For example, DNR-loaded magnetic nanoparticles of Fe 3 O 4 can overcome MDR. 29 In another study, nickel nanoparticles have shown increased cancer cell membrane permeability. 7 However, the use of nanoparticles as DNR sensitizers has been of major concern due to their known toxicity.…”

Section: Discussionmentioning

confidence: 97%

Nano-hole induction by nanodiamond and nanoplatinum liquid, DPV576, reverses multidrug resistance in human myeloid leukemia (HL60/AR)

Ghoneum¹,

Sharma²,

Gimzewski³

2013

IJN

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Abstract:Recently nanoparticles have been extensively studied and have proven to be a promising candidate for cancer treatment and diagnosis. In the current study, we examined the chemo-sensitizing activity of a mixture of nanodiamond (ND) and nanoplatinum (NP) solution known as DPV576, against multidrug-resistant (MDR) human myeloid leukemia (HL60/AR) and MDR-sensitive cells (HL60). Cancer cells were cultured with different concentrations of daunorubicin (DNR) (1 × 10 −9 -1 × 10 −6 M) in the presence of selected concentrations of DPV576 (2.5%-10% v/v). Cancer cell survival was determined by MTT assay, drug accumulation by flow cytometry and confocal laser scanning microscopy (CLSM), and holes and structural changes by atomic force microscopy (AFM). Co-treatment of HL60/AR cells with DNR plus DPV576 resulted in the reduction of the IC 50 to 1/4th. This was associated with increased incidences of holes inside the cells as compared with control untreated cells. On the other hand, HL60 cells did not show changes in their drug accumulation post-treatment with DPV576 and DNR. We conclude that DPV576 is an effective chemo-sensitizer as indicated by the reversal of HL60/AR cells to DNR and may represent a potential novel adjuvant for the treatment of chemo-resistant human myeloid leukemia.

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Daunorubicin-loaded magnetic nanoparticles of Fe3O4 overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

Cited by 30 publications

References 44 publications

Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells

Synthesis and antitumor efficacy of daunorubicin-loaded magnetic nanoparticles

Nano-hole induction by nanodiamond and nanoplatinum liquid, DPV576, reverses multidrug resistance in human myeloid leukemia (HL60/AR)

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