Daucus carota Cell Cultures: Polyamines and Effect of Polyamine Biosynthesis Inhibitors in the Preembryogenic Phase and Different Embryo Stages

Mengoli, Marisa; Bagni, Nello; Luccarini, G.; Ronchi, Vittoria Nuti; Serafini-Fracassini, Donatella

doi:10.1016/s0176-1617(89)80001-x

Cited by 64 publications

(29 citation statements)

References 21 publications

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“…Martinez et al (2000) showed that the addition of combined Put and Spd in a hormone-free medium improved gynogenesis in onion, while the addition of Put alone had no significant effect. These results are in agreement with the influence of Spd in embryogenesis reported for carrot cell cultures (Mengoli et al 1989) or in maize androgenesis (Santos et al 1995). The distinct PA profiles that we observed between CG12 on one hand and PG49 and CG11 on another hand, could be due to distinct PA regulations.…”

Section: Discussionsupporting

confidence: 93%

“…Such findings would corroborate, in a distinct system of embryogenesis, the involvement of the ADC pathway, what has been so far demonstrated in somatic embryogenesis (Feirer et al 1984, Mengoli et al 1989. In tobacco androgenesis, Garrido et al (1995) observed that ADC and ODC activities decreased during embryogenesis induction, but ADC activity was required for the embryo formation itself.…”

Section: Discussionsupporting

confidence: 83%

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Polyamines are involved in the gynogenesis process in onion

Geoffriau

Kahane

Martin-Tanguy

2006

Physiologia Plantarum

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Haploidization in onion (Allium cepa L.) using immature flower buds simulates zygotic embryogenesis with no fecundation. In order to know the involvement of polyamines (PAs) in this process, we determined the concentration of endogenous PAs in flower buds and experimented the addition of various combinations of PA molecules in the medium. At the inoculation stage, high levels of free and conjugated spermidine and low putrescine þ hydroxyputrescine/spermidine þ spermine ratio characterized the highest responsive varieties. During in vitro culture, high levels of putrescine and its derivatives characterized the lowest responsive varieties, whereas high levels of spermidine and spermine characterized responsive varieties. The putrescine þ hydroxyputrescine þ homospermidine/spermidine þ spermine ratio remained low in responsive varieties. The addition of spermidine or spermine (2 · 10 À3 M) to the culture medium improved significantly the embryo production. Our results suggest that the arginine decarboxylase pathway is involved in PA biosynthesis during the in vitro culture of flower buds. Our study showed that specific ratios of PAs are required for successful gynogenesis in onion.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 83%

Polyamines are involved in the gynogenesis process in onion

Geoffriau

Kahane

Martin-Tanguy

2006

Physiologia Plantarum

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“…Canavanine inhibits the incorporation of arginine into putrescine in tuber slices of Helianthus tuberosus (L.) (Bagni et al, 1981) and inhibits arginine decarboxylase (ADC) (Mengoli et al, 1989). Canaline and canavanine are approximately ten times as inhibitory as DFMO or DFMA in leafy spurge hypocotyl segments; being effective at ca.…”

Section: Discussionmentioning

confidence: 99%

Polyamines, auxins and organogenesis in leafy spurge (Euphorbia esula L.)

Davis¹

1997

Journal of Plant Physiology

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Summary:Canaline and canavanine (inhibitors of polyamine biosynthesis) effects on root and shoot formation were determined in vitro for etiolated hypocotyl segments of leafy spurge (Euphorbia esula L.). Canaline (an ornithine analogue) at 10 to 100 µmol/L inhibited root and shoot formation. The simultaneous application of putrescine did not reverse canaline-induced inhibition of root formation. Ornithine, a precursor of putrescine via the ornithine decarboxylase pathway, unexpectedly inhibited root formation. Canavanine (an arginine analogue) at 30 to 100 µmol/L inhibited root and/or shoot formation. Inhibition of root formation by canavanine was not reversed by agmatine or putrescine, but was partially reversed by arginine. Indoleacetic acid (IAA) inhibited shoot formation, stimulated root formation, and partially reversed the inhibition of root formation by either canaline or canavanine. IAA generally increased endogenous levels of free polyamines in controls and canaline-or canavanine-treated tissues, but these changes were small and probably had little effect on organogenesis. IAA also partially reversed the inhibition of root (but not shoot) formation by arcaine and pentamidine, inhibitors of polyamine action in mammals. The roles of the polyamines in plants is not clear, but the polyamines may play only minor roles in root and shoot formation.

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“…For example, DFMO (1 mM) had no effect on development and growth of grapevine microcuttings in vitro, as opposed to DFMA, which inhibited development (Martin-Tanguy and Carre 1993). In carrot (Daucus carota L.), 5 mM DFMO inhibited ADC and ODC in vitro but not in vivo (Mengoli et al 1989), and 5 mM DFMO enhanced somatic embryogenesis in carrot cell suspension cultures (Nissen and Minocha 1993). Root growth was inhibited by DFMO in germinating seeds of Arabidopsis thaliana (Mirza and Bagni 1991).…”

Section: Introductionmentioning

confidence: 99%

2,4-Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

Davis¹

1997

Physiol Plant

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Abstract:Difluoromethylornithine (DFMO) is a well known inhibitor of putrescine biosynthesis that has been reported to interact in varying ways with auxins such as indoleacetic acid (IAA) and 2,4-dichlorophenoxy acetic acid (2,4-D). In the present report DFMO is shown to inhibit root formation in isolated hypocotyl segments of Euphorbia esula L. (leafy spurge) grown in the dark on solidified nutrient media in Petri dishes. Shoot formation was only slightly inhibited by DFMO and only on media with salts and vitamins diluted 10-fold. 2,4-D inhibited both root and shoot formation in full strength or diluted media. Simultaneous application of both compounds resulted in partial reversal of root inhibition, but only at 450 nM 2,4-D, the highest concentration used. In both media IAA also partially reversed DFMO effects on root formation. The effects of DFMO, 2,4-D or IAA on root (or shoot) formation do not appear to be closely related to endogenous content of the polyamines determined by high performance liquid chromatography. Abbreviations:DFMA, DL-α-difluoromethylarginine; DFMO, DL-α-difluoromethy-

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Daucus carota Cell Cultures: Polyamines and Effect of Polyamine Biosynthesis Inhibitors in the Preembryogenic Phase and Different Embryo Stages

Cited by 64 publications

References 21 publications

Polyamines are involved in the gynogenesis process in onion

Polyamines are involved in the gynogenesis process in onion

Polyamines, auxins and organogenesis in leafy spurge (Euphorbia esula L.)

2,4-Dichlorophenoxy acetic acid and indoleacetic acid partially counteract inhibition of organogenesis by difluoromethylornithine

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