Daucosterol suppresses dextran sulfate sodium (DSS)-induced colitis in mice

Jang, Jin; Kim, Su-Man; Yee, Su-Min; Kim, Eun‐Mi; Lee, Eun-Hee; Choi, Hyungsub; Lee, Young-Sil; Yang, Won‐Kyung; Kim, Han-Young; Kim, Kun-Hoae; Kang, Hyung‐Sik; Kim, Seung-Hyung

doi:10.1016/j.intimp.2019.03.062

Cited by 24 publications

(12 citation statements)

References 19 publications

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“…Our results demonstrated a significant anti-inflammatory activity of selagin, which has been also previously demonstrated in vivo [60]. Immunoregulatory activity of daucosterol was also found both in vitro and in vivo [61][62][63]. Selaginella-unique biflavonoids also exhibited anti-inflammatory activity by reversing the TNF-α signaling pathway [64].…”

Section: Discussionsupporting

confidence: 84%

Comparison of Chemical Composition and Biological Activities of Eight Selaginella Species

et al. 2020

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Selaginella P. Beauv. is a group of vascular plants in the family Selaginellaceae Willk., found worldwide and numbering more than 700 species, with some used as foods and medicines. The aim of this paper was to compare methanolic (MeOH) and dichloromethane (DCM) extracts of eight Selaginella species on the basis of their composition and biological activities. Six of these Selaginella species are underinvestigated. Using ultra-high performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) analysis, we identified a total of 193 compounds among the tested Selaginella species, with flavonoids predominating. MeOH extracts recovered more constituents that were detected, including selaginellins, the occurrence of which is only typical for this plant genus. Of all the tested species, Selaginellaapoda contained the highest number of identified selaginellins. The majority of the compounds were identified in S. apoda, the fewest compounds in Selaginellacupressina. All the tested species demonstrated antioxidant activity using oxygen radical absorption capacity (ORAC) assay, which showed that MeOH extracts had higher antioxidant capacity, with the half maximal effective concentration (EC50) ranging from 12 ± 1 (Selaginellamyosuroides) to 124 ± 2 (Selaginellacupressina) mg/L. The antioxidant capacity was presumed to be correlated with the content of flavonoids, (neo)lignans, and selaginellins. Inhibition of acetylcholinesterase (AChE) was mostly discerned in DCM extracts and was only exhibited in S. myosuroides, S. cupressina, Selaginellabiformis, and S. apoda extracts with the half maximal inhibitory concentration (IC50) in the range of 19 ± 3 to 62 ± 1 mg/L. Substantial cytotoxicity against cancer cell lines was demonstrated by the MeOH extract of S. apoda, where the ratio of the IC50 HEK (human embryonic kidney) to IC50 HepG2 (hepatocellular carcinoma) was 7.9 ± 0.2. MeOH extracts inhibited the production of nitrate oxide and cytokines in a dose-dependent manner. Notably, S. biformis halved the production of NO, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 at the following concentrations: 105 ± 9, 11 ± 1, and 10 ± 1 mg/L, respectively. Our data confirmed that extracts from Selaginella species exhibited cytotoxicity against cancer cell lines and AChE inhibition. The activity observed in S. apoda was the most promising and is worth further exploration.

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Section: Discussionsupporting

confidence: 84%

Comparison of Chemical Composition and Biological Activities of Eight Selaginella Species

et al. 2020

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“…All of the four compounds have notable pharmaceutical activities. The effects of daucosterol have been reported for the suppression of cancer, promotion of neural stem cell proliferation, induction of Th1 immune response, and especially suppression of acute enteritis [42]. As mentioned in introduction, only the male was applied in clinical practice for bacillary dysentery and acute enteritis.…”

Section: Discussionmentioning

confidence: 99%

1H NMR Combined with Multivariate Statistics for Discrimination of Female and Male Flower Buds of Populus tomentosa

et al. 2021

Molecules

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1H Nuclear Magnetic Resonance (1H NMR) combined with multivariate statistics was adopted to discriminate female and male flower buds of Populus tomentosa in the study. Samples of 11 female and 16 male flower buds of P. tomentosa were collected in Beijing, China. 1H NMR spectra were acquired on a 400 MHz spectrometer. In total, 30 chemical compounds were identified with standards and literature according to chemical shifts, peak areas, and multiplicity. Principal component analysis (PCA), hierarchical clustering analysis (HCA), and supervised orthogonal partial least squares-discriminant analysis (OPLS-DA) were applied to discriminate female and male flower buds. An apparent grouping trend (R2X, 0.809; Q2, 0.903) between female and male groups was exhibited with PCA and HCA. The two groups were also well discriminated with OPLS-DA (R2X, 0.808; R2Y, 0.976; Q2, 0.960). Combined with variable importance in projection (VIP) > 1.0 and p < 0.05 of OPLS-DA, it was found that the content of daucosterol, β-sitosterol, ursolic acid, and betulonic acid in male group was higher than that in female, which should be the key differences of chemical constituents in female and male flower buds of P. tomentosa. The study demonstrated that 1H NMR combined with multivariate statistics could be used to discriminate female and male plants and clarify differences, which provided a novel method to identify the gender of dioecious plants.

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“…Six-week-old, healthy, specific pathogen-free (SPF) female C57BL/6 mice weighing approximately (20.0 ± 1.0) g were obtained from Beijing Vital River Laboratory Animal Technology Co. We used the female sex only, as female C57BL/6 mice have been reported to be more susceptible to UC modeling in the literature 69 . All the mice were acclimatized under SPF conditions, exposed to 12 h cycles of light and dark, and fed with standard rodent chow and water ad libitum in clear cages for one week.…”

Section: Methodsmentioning

confidence: 99%

Porphyromonas gingivalis exacerbates ulcerative colitis via Porphyromonas gingivalis peptidylarginine deiminase

et al. 2021

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Ulcerative Colitis (UC) has been reported to be related to Porphyromonas gingivalis (P. gingivalis). Porphyromonas gingivalis peptidylarginine deiminase (PPAD), a virulence factor released by P. gingivalis, is known to induce inflammatory responses. To explore the pathological relationships between PPAD and UC, we used homologous recombination technology to construct a P. gingivalis strain in which the PPAD gene was deleted (Δppad) and a Δppad strain in which the PPAD gene was restored (comΔppad). C57BL/6 mice were orally gavaged with saline, P. gingivalis, Δppad, or comΔppad twice a week for the entire 40 days (days 0−40), and then, UC was induced by dextran sodium sulfate (DSS) solution for 10 days (days 31−40). P. gingivalis and comΔppad exacerbated DDS-induced colitis, which was determined by assessing the parameters of colon length, disease activity index, and histological activity index, but Δppad failed to exacerbate DDS-induced colitis. Flow cytometry and ELISA revealed that compared with Δppad, P. gingivalis, and comΔppad increased T helper 17 (Th17) cell numbers and interleukin (IL)-17 production but decreased regulatory T cells (Tregs) numbers and IL-10 production in the spleens of mice with UC. We also cocultured P. gingivalis, Δppad, or comΔppad with T lymphocytes in vitro and found that P. gingivalis and comΔppad significantly increased Th17 cell numbers and decreased Treg cell numbers. Immunofluorescence staining of colon tissue paraffin sections also confirmed these results. The results suggested that P. gingivalis exacerbated the severity of UC in part via PPAD.

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Daucosterol suppresses dextran sulfate sodium (DSS)-induced colitis in mice

Cited by 24 publications

References 19 publications

Comparison of Chemical Composition and Biological Activities of Eight Selaginella Species

Comparison of Chemical Composition and Biological Activities of Eight Selaginella Species

1H NMR Combined with Multivariate Statistics for Discrimination of Female and Male Flower Buds of Populus tomentosa

Porphyromonas gingivalis exacerbates ulcerative colitis via Porphyromonas gingivalis peptidylarginine deiminase

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