Datura Stramonium leaves extract Silver Nanoparticles regulates PINK1 gene in Parkinson’s disease model of Drosophila melanogaster

Abstract

In recent years, several eco-friendly processes for the synthesis of silver nanoparticles (AgNPs) are increasing; they have tremendous applications in the field of diagnosis and therapeutic values for various diseases /disorders. In this article the green synthesis of Datura stramonium leaves extract silver nanoparticles (DS-AgNPs) was carried out and are characterized for different properties like reduction of Ag + to Ag◦, absorption maxima, functional group of secondary metabolites, crystalline structure, morphology, and size by using UV-Vis spectroscopy, fourier transform infrared spectroscopy (FITR) X-ray spectroscope (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM) respectively. Further the effects of DS-AgNPs on WT Park TM3 SB and WI Park 25 Parkinson’s mutant Drosophila melanogaster was assessed by the Negative geotaxis assay and PINK 1 gene expression. Treatment with DS-AgNPs exhibited increased geotaxis behavior and with a positive survival percentage, finally PINK1 was found to be down regulated as the treatment concentrations increased in WI Park 25 mutant but up regulated in WT Park TM3 SB mutant PD model. Although more molecular mechanism needs to be understood, however the use of DS-AgNPs can be a promising treatment strategy for the PD.

Synthesis of Green Engineered Silver Nanoparticles through Urtica dioica: An Inhibition of Microbes and Alleviation of Cellular and Organismal Toxicity in Drosophila melanogaster

“…This finding is supported by a prior study that revealed that plant constituents dramatically increased cell viability. Due to the activation of antioxidant defense systems or the existence of bioactive components that quench free radicals, UD-AgNPs have protective properties [ 68 ].…”

Plant-mediated synthesis of silver nanoparticles: unlocking their pharmacological potential–a comprehensive review

“…An early-onset variant of autosomal recessive Parkinson’s disease is caused by mutations in the PINK1 gene ( Deas et al, 2009 ). In a study by Shivanna et al (2022) , it was discovered that AgNPs made from Datura stramonium leaf extract of spherical shape and 39–59 nm size have the power to alter the regulation of the PINK1 gene. It was found that after being fed with the AgNPs, the flies had higher geotaxis behavior and a consequently favorable survival percentage in the investigation, which was conducted in vivo on a mutant Parkinson’s Disease model of Drosophila melanogaster .…”