Datumetine Preferentially Upregulates N-methyl-D-aspartate Receptor Signalling Pathways in Different Brain Regions of Mice

Abstract

Background: We earlier reported that datumetine possesses binding affinity with NMDAR and that 14-day exposure to datumetine altered NMDAR signalling by mimicking glutamate toxicity. Here, we investigated the potential neuroprotective effect of a single shot of a low dose of datumetine administration in BALB/c mice. Results: Relative to Veh, datumetine downregulate the expression of CamKIIα in the hippocampus and PFC but not in the cerebellum, CREB was also upregulated in the PFC only, but pCREB was also upregulated in all the three brain regions observed, while BDNF was only upregulated in both hippocampus and PFC of Datumetine relative to Veh. MK-801 on the other hand reversed some of the effects of datumetine in the brain regions observed. No major histological alterations were observed in the different brain regions immunohistochemically. Conclusion: We conclude that a single low dose of datumetine moderately enhances NMDAR activity. This showed the neuroprotective potentials of low datumetine exposure.

Alterations in surface-based amplitude of low-frequency fluctuations primary open-angle glaucoma link to neurotransmitter profiling and visual impairment severity

“…The dopamine transporter (DAT) demonstrates signi cant performance solely in fALFF, which plays a crucial role in dopamine neurotransmission, and dysfunction in DAT function can contribute to pathological conditions associated with hyperdopaminergic [93] . Gene-modi ed rodents lacking DAT exhibit elevated levels of striatal dopamine, leading to behavioral abnormalities and cognitive de cits [94] .…”