Date Pit Carbon Dots Induce Acidic Inhibition of Peroxidase and Disrupt DNA Repair in Antibacteria Resistance

Abstract

Carbon nanodots (C‐dots) are emerging as a new type of promising agent in anticancer, imaging, and new energy. Reports as well as the previous research indicate that certain C‐dots can enhance targeted cancer therapy. However, in‐depth mechanisms for such anticancer effect remain unclear. In this work, treatment provided by the date pit‐derived C‐dots, exhibits significant DNA damage; Annexin V/7‐AAD‐mediated apoptosis, and G2/M cell cycle arrest in prostate cancer cells. The application of C‐dots to the cell generally leads to acidulation of the cell medium, cooperated with membrane compact. The date pit‐derived C‐dots are observed inhibiting the horseradish peroxidase. Moreover, the C‐dots disrupt likely through nucleotide excision DNA repair at low dose during DNA ligation step suggesting the antimicrobial effect and targeting Pim‐1, EGFR, mTOR, and DNA damage pathways in cancer cells. For the first time the detailed and novel mechanisms underlying the C‐dots, derived from the date‐pit, as an efficient, low‐cost, and green nanomaterial are reveled for cancer therapy and anti‐infection.

Network Pharmacology with Experimental Investigation of the Mechanisms of Rhizoma Polygonati against Prostate Cancer with Additional Herbzymatic Activity

“…The cell survival assay was performed by treating cells for 3 days with the concentration indicated in the figure legend, followed by fixation and staining with crystal violet as described previously. 41 For the soft agar assay, cells were plated in 6-well plates with 6% bottom agarose and 3% top agarose (Sigma-Aldrich) and treated with the vehicle or RP extract at 0.5 mg/mL during plating when cooling down using the standard protocol of the soft agar assay. About 4 weeks post-treatment, the colonies were counted and inhibition efficiencies were calculated by percentage.…”

“…AFM and scanning electron microscopy (SEM) sample preparations were described previously. 41 In detail, for SEM analysis, herbal RP extracts were put on foil papers and subjected to quick drying in air. 10 Finally, the samples were investigated using a Zeiss SEM microscope as described previously.…”

“…10 Finally, the samples were investigated using a Zeiss SEM microscope as described previously. 41 Statistical Analysis. Student's T test was the statistical analysis method to check the significance, and p < 0.05 or 0.01 was applied for analysis.…”

“…Atomic force microscopy (AFM) analysis was carried out with a Smart SPM 1000. AFM and scanning electron microscopy (SEM) sample preparations were described previously . In detail, for SEM analysis, herbal RP extracts were put on foil papers and subjected to quick drying in air .…”

“…In detail, for SEM analysis, herbal RP extracts were put on foil papers and subjected to quick drying in air . Finally, the samples were investigated using a Zeiss SEM microscope as described previously …”