Dataset on LC-Q-TOF/MS tentative identification of phytochemicals in the extract of Vernonia amygdalina leaf through positive ionization

Alara, Oluwaseun Ruth; Abdurahman, Nour Hamid; Ukaegbu, Chinonso Ishmael; Hassan, Zulkafli; Kabbashi, Nassereldeen Ahmed

doi:10.1016/j.dib.2018.10.159

Cited by 16 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gradient elution was performed with 70 % A and 30 % B (0.00 min), 50 % A and 50 % B (1.04 min), 50 % A and 50 % B (2.08 min), 30 % A and 70 % B (5.21 min), and 70 % A and 30 % B (5.56 min) with a flow rate of 0.4 mL min −1 and injection volume of 20 µL. A SYNAPT mass spectrometer (Waters) coupled with electrospray ionization operated in both positive‐ and negative‐ion modes, mass range of m / z 100–1000, spray voltage of 4 keV, column temperature of 40 °C, sample temperature of 15 °C, gas flow of 0.5 mL min −1 , desolvation temperature of 550 °C, and desolvation gas flow rate of 800 L h −1 was used for identification , .…”

Section: Methodsmentioning

confidence: 99%

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

2019

Self Cite

View full text Add to dashboard Cite

Phenolic compounds are secondary metabolites that are mainly responsible for different pharmacological activities of plant extracts. In this study, microwaveassisted extraction (MAE) variables were optimized for the extraction of phenolic compounds from Chromolaena odorata leaves by using a Box-Behnken design of response surface methodology. The concentration of polyphenols and functional characteristics of the extracts were identified by using liquid chromatography quadrupolar time-of-flight mass spectrometry and FTIR analyses, respectively. The obtained results indicated the MAE conditions for the optimal recoveries of the total phenolic content and total flavonoid content from C. odorata leaves. The results reveal that the C. odorata leaf is enriched with phenolic compounds.

show abstract

Section: Methodsmentioning

confidence: 99%

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In order to address the low fragmentation efficiency of in‐source CID, the high collision energy characteristic of MS ALL technique was applied to LC–Q‐TOF‐MS. LC–Q‐TOF‐MS is a hybrid MS technology consisted of a unit mass resolution quadrupole (Q1), a second quadrupole (Q2), and a TOF mass analyzer with high resolution [211–218]. In the acquisition procedure of MS ALL technique, all the precursor ions are allowed to go through Q1 freely and fragmented in Q2.…”

Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning

confidence: 99%

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Zhang

Song

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

Polyethylene glycols are synthetic polymers composed of repeating oxyethylene subunits, which have been known for non-toxic, non-immunogenic, non-antigenic, good solubility in water and therefore approved for pharmaceutical applications. Recently, attachment or amalgamation of polyethylene glycols to therapeutic small molecules, peptides, proteins, or nanoparticles has become a mature technology for the sake of improving their pharmacokinetic and pharmacological profiles, also referred to as PEGylation. By comparison, there are only a few PEGylated pharmaceuticals have been registered for further clinical trials and even less was approved for marketing. High failure rate of PEGylated pharmaceuticals in pre-clinical and clinical trials could be majorly attributed to their unclear pharmacokinetic behaviors. Therefore, the in vivo fate of the PEGylated pharmaceuticals for the various routes of administration needs to be thoroughly investigated An accurate in vivo pharmacological study thereof highly depends on the precise detection of polyethylene glycols as well as their fragments in biological matrixes. The goal of this review is to highlight the analytical methods that were developed and applied to evaluate the polyethylene glycols in pharmaceutical ingredients and excipients, which bring us closer to bridging

show abstract

“…In another study on the effect of V. amygdalina leaves extracts on blood glucose of diabetic rats, the results showed that decrease in blood glucose after administration of the extracts may be associated with the presence vitamins, phytochemicals, and other nutrients in the extracts [27,28]. Thus, the presence of trigonelline and caffeoylquinic acid derivative might be attributed to the anti-diabetic activity of the extracts [29,30].…”

mentioning

confidence: 97%

Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia

Alara¹,

Abdurahman²

2019

jrp

Self Cite

View full text Add to dashboard Cite

Diabetes has been characterized by transmuted lipids, hyperglycemia, proteins, and carbohydrates metabolism that can affect the quality of life. Although there are quite several synthetic drugs used in managing this disease, however, due to their side effects, the use of natural sources is imperative. This study focused on the in-vitro inhibitory activity of Vernonia amygdalina leaf grown in Malaysia using both α-amylase and α-glucosidase assays. The quantities of mineral elements in the plant extracts through microwave-assisted and Soxhlet extraction methods were as well examined through inductively coupled plasma mass spectrometry (ICP-MS) analysis. The extract of V. amygdalina leaf showed the highest dose-dependent effect of 97.28 and 98.08% of inhibition at 1.0 mg/mL having IC50 values of 0.51 mg/mL and 0.49 mg/mL against α-amylase and α-glucosidase enzymes, respectively. The inhibitory effects of the extract were higher than acarbose (a standard drug). Moreover, the results showed that higher quantities of mineral elements (potassium, magnesium, and calcium) and a lesser quantity of chromium in the extracts through microwave-assisted extraction (MAE) indicate that the technique possesses a higher capacity in obtaining higher mineral nutrients over Soxhlet extraction and more anti-diabetic activity of the extract. Thus, the extract exhibited an effective anti-diabetic activity which implies that it potentially serves as a natural source for managing diabetes.

show abstract

Dataset on LC-Q-TOF/MS tentative identification of phytochemicals in the extract of Vernonia amygdalina leaf through positive ionization

Cited by 16 publications

References 3 publications

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals

Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia

Contact Info

Product

Resources

About