Dataset of Nematostella vectensis Hsp70 isoform interactomes upon heat shock

Abstract

Nematostella vectensis is an estuarine sea anemone that has emerged as a model species to characterize molecular responses to physiological stressors due to its exposure to diverse, extreme abiotic conditions. In marine cnidarians, Hsp70 proteins can be effective biomarkers to determine mechanisms of physiological acclimation and evolutionary adaptations to environmental stress: a pressing issue as concerns about climate change grow. Here we show the results of affinity purification mass spectrometry of three Nematostella vectensis Hsp70 isoforms, NvHsp70A, B and D when expressed in untreated and heat shocked yeast cells lacking their native Hsp70s. We identified a total of 1031 interactors for the three NvHsp70 isoforms, 549 or which were shared. NvHsp70 isoform interactions altered substantially under heat stress with 17% of NvHsp70A, 51% of NvHsp70B and 20% of NvHsp70D interactions increasing after exposure to 39 °C for 2 hours. For further interpretation of the data presented in this article, please see the research article “Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock”.

The C-terminal domain of Hsp70 is responsible for paralog-specific regulation of ribonucleotide reductase

“…The copyright holder for this preprint this version posted February 8, 2022. ; https://doi.org/10.1101/2022.02.08.479504 doi: bioRxiv preprint 10 A challenge in understanding the differential role of the Ssa paralogs is a lack of verified client proteins. While several Hsp70 interactomes have been published under differing conditions and phosphorylation site mutations, validation of these and their cellular effect is still under investigation [33,[43][44][45][46][47]. Excellent attempts to dissect the roles of Ssa paralogs include the well-established Hsp90 client (Ste11) and a non-yeast client, v-Src in addition to the yeast prions [URE3] and [PSI + ] [21].…”

The C-terminal domain of Hsp70 is responsible for paralog-specific regulation of ribonucleotide reductase

“…A challenge in understanding the differential role of the Ssa paralogs is a lack of verified client proteins. While several Hsp70 interactomes have been published under differing conditions and phosphorylation site mutations, validation of these and their cellular effect is still under investigation [ 37 , 47 – 51 ]. Excellent attempts to dissect the roles of Ssa paralogs include the well-established Hsp90 client (Ste11) and a non-yeast client, v-Src in addition to the yeast prions [ URE3 ] and [ PSI + ] [ 23 , 25 , 52 , 53 ].…”

Chromatin Dynamics and Gene Expression Response to Heat Exposure in Field-Conditioned versus Laboratory-Cultured Nematostella vectensis

“…While field-conditioned N. vectensis responde by regulating damage repair pathways, lab-cultured N. vectensis responded throug amino acid metabolic pathways. Today, N. vectensis is accepted as a model organism with well-developed genomic tools that investigators have used to characterize mechanisms of diverse stress responses [11][12][13][14]. Nonetheless, such studies raise the question of the extent to which laboratory observations are representative of the biological responses that would be manifested by natural animal populations.…”