Dataset of mRNA levels for dopaminergic receptors, adrenoceptors and tyrosine hydroxylase in lymphocytes from subjects with clinically isolated syndromes

Abstract

This data article presents a dataset of mRNA levels for dopaminergic receptors, adrenoceptors and for tyrosine hydoxylase, the rate-limiting enzyme in the synthesis of catecholamines, in peripheral blood mononuclear cells as well as in CD4+ T effector and regulatory cells from subjects with clinically isolated syndromes (CIS), which is a first episode of neurological disturbance(s) suggestive of multiple sclerosis. CIS subjects are divided into two groups according to their eventual progression, after 12 months from CIS, to clinically established multiple sclerosis. The data reported are related to the article entitled "Dopaminergic receptors and adrenoceptors in circulating lymphocytes as putative biomarkers for the early onset and progression of multiple sclerosis" (M. Cosentino, M. Zaffaroni, M. Legnaro, R. Bombelli, L. Schembri, D. Baroncini, A. Bianchi, R. Clerici, M. Guidotti, P. Banfi, G. Bono, F. Marino, 2016) [1].

Dopaminergic inhibition of human neutrophils is exerted through D1‐like receptors and affected by bacterial infection

“…Data obtained from the ex vivo animal model support the hypothesis of a direct involvement of DR‐operated pathways in the observed ability of DA to affect PMN functions and of a predominant involvement of the D 1 ‐like DR family (in particular DR D5) in agreement with other studies showing a dysregulation of DR in different human diseases such as MS or PD [11, 15, 18, 19, 21, 53]. In PMN isolated from the bone marrow of DRD5KO mice we showed that DA lacked the ability to prevent fMLP‐induced migration while, on the contrary, this ability was preserved in cells from WT mice; additionally, the key functional role of dopaminergic pathways was suggested also by the reduced PMN count in spleens from DRD5KO mice.…”

“…Data obtained from the ex vivo animal model support the hypothesis of a direct involvement of DR-operated pathways in the observed ability of DA to affect PMN functions and of a predominant involvement of the D 1 -like DR family (in particular DR D5) in agreement with other studies showing a dysregulation of DR in different human diseases such as MS or PD [11,15,18,19,21,53].…”

“…On the contrary, upon recovery the dopaminergic inhibitory tone is re‐established as part of a general anti‐inflammatory programme in order to limit excessive inflammation and eventual tissue damage. DA dysregulation indeed occurs in several human diseases in cells of adaptive immunity [11, 15, 53], as well as of innate immunity. Interestingly, studies on animal models show that infective conditions reduce DAβ hydroxylase activity [54] and that viral disease such as HIV infection can affect DA pathways [55].…”

“…The relevance of DR dysregulation for example is established in adaptive immune cells in CNS diseases where DRD5 mRNA expression in isolated regulatory T lymphocytes are predictive of MS in Pts with clinically isolated syndrome [53] and in PBMC are predictive of MS progression [16]. Moreover, in PD D1-like DR membrane expression in T lymphocytes is downregulated in comparison to cells from HS [11].…”