Database screening of herbal monomers regulating autophagy by constructing a "disease-gene-drug" network

Hao, Chenjun; Yang, Zhengpeng; Gao, Bo; Lu, Ming; Meng, Xianmin; Qiao, Xin; Xue, Dongbo; Zhang, Weihui

doi:10.1186/1472-6882-14-466

Cited by 2 publications

(3 citation statements)

References 70 publications

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“…A disease–gene–drug network was constructed based on human protein reference database and the Gene Expression Omnibus database. Then, 30 herbal monomers were screened that could regulate 7 autophagic genes simultaneously, indicating their potential role in autophagy …”

Section: Systems Biology Network Approaches Of Autophagymentioning

confidence: 99%

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Deciphering the Rules of in Silico Autophagy Methods for Expediting Medicinal Research

et al. 2019

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Autophagy is a highly evolutionarily conserved cellular catabolic process responsible for degradation of damaged organelles and long-lived proteins. It is not surprising that scientific interest in the connection of autophagy and diseases has been growing. Over the past 2 decades, many new experimental approaches have been emerging in the field of targeting autophagy for medicinal research. Interestingly, in addition to experimental methods, a number of databases, Web servers, mathematics models, omics approaches, and systems biology network approaches related to autophagy have become available online, which may be collectively considered to be "in silico autophagy methods" for expediting current medicinal research. Thus, we have summarized a series of relevant in silico autophagy approaches for promoting the most appropriate usage of these resources for potential therapeutic purposes.

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Section: Systems Biology Network Approaches Of Autophagymentioning

confidence: 99%

“…Then, 30 herbal monomers were screened that could regulate 7 autophagic genes simultaneously, indicating their potential role in autophagy. 49 Network Approaches for Autophagy-Related Diseases. Recently, the microRNA-regulated autophagic pathways have been identified in plant lectin-induced cancer cell death.…”

Section: ■ Omics-based Analyses Of Autophagymentioning

confidence: 99%

Deciphering the Rules of in Silico Autophagy Methods for Expediting Medicinal Research

et al. 2019

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“…Autophagy is a highly conserved evolutionary cellular process, providing a membrane-dependent mechanism for the sequestration, transport, and lysosomal turnover of subcellular components [23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Rutin Reverses Abnormal Autophagy in Hypoxic Pulmonary Arterial Hypertension via Regulation of Mitofusin 1

Huang

Wang

Song

et al. 2021

Preprint

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Background:Recently, rutin, a citrus flavonoid occurring in many plants, has been found to be beneficial in preventing hypoxia-induced pulmonary arterial smooth muscle cells (PASMCs) from proliferating via scavenged reactive oxygen species (ROS). However, rutin’s underlying mechanism of action against PASMCs in the context of hypoxia is still unclear. Autophagy, the main intracellular degradation and recycling process, exerts a critical adaptive effect on the pathological angiogenesis associated with hypoxic pulmonary arterial hypertension (HPAH) by removing damaged mitochondria and regulating ROS production and cell proliferation. It would be useful to identify the role of rutin and its interaction with autophagy in exerting protective effects against HPAH.Methods:We chose 21 SD rats and randomly and equally divided them into three groups of normoxia, hypoxia, and hypoxia + rutin. At the end of the exposure period, we measured the right ventricular systolic pressure (RVSP), the weight of right ventricle (RV), and the ratio of RV weight to left ventricular (LV) weight plus septum (RV/LV+S) of each rat. PASMCs of the three groups of rats were isolated and cultured, and the effect of rutin on autophagy-related protein expression under hypoxia was analyzed using immunofluorescence analysis, transmission electron microscopy, western blot (WB) analysis, and siRNA design and transfection.Results:We found RVSP, RV/LV+S, and pulmonary artery wall thickness were reduced by rutin in the pulmonary arterial hypertension (PAH) animal model. WB results showed that rutin regulated expression of autophagy-related proteins. Moreover, rutin downregulated Mitofusin 1 (Mfn1) over-expression induced by hypoxia. But when Mfn1 was silenced, there was little difference in the expressions of beclin-1 (BECN-1), and other marker proteins with or without rutin.Conclusions:Rutin suppressed the abnormal autophagy of hypoxia-induced PASMCs via the regulation of the target, Mfn1. This revealed the protective effect of rutin on vascular remodeling caused by hypoxia and demonstrated how rutin could slow down the development of HPAH.

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Database screening of herbal monomers regulating autophagy by constructing a "disease-gene-drug" network

Cited by 2 publications

References 70 publications

Deciphering the Rules of in Silico Autophagy Methods for Expediting Medicinal Research

Deciphering the Rules of in Silico Autophagy Methods for Expediting Medicinal Research

Rutin Reverses Abnormal Autophagy in Hypoxic Pulmonary Arterial Hypertension via Regulation of Mitofusin 1

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