Data on SD-OCT image acquisition, ultrastructural features, and horizontal tissue shrinkage in the porcine retina

Xie, Wankun; Zhao, Min; Tsai, Shu‐Huai; Burkes, William L.; Potts, Luke B.; Xu, Wei; Payne, H. Ross; Hein, Travis W.; Li, Kuo; Rosa, Robert H.

doi:10.1016/j.dib.2018.10.123

Cited by 5 publications

(32 citation statements)

References 2 publications

(2 reference statements)

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“…A subtle decrease in the grayscale reflectivity is apparent in Figure 1C corresponding to the same location. TEM revealed various cell types in the middle of the IPL, including endothelial cells (Xie et al, 2018 [Figure 2A]), fibrous astrocytes (Xie et al, 2018 [Figure 2B]), amacrine cells (Xie et al, 2018 [Figure 3A]), and Müller cells (Xie et al, 2018 [Figure 3B]).…”

Section: Resultsmentioning

confidence: 99%

“…Histologic analysis of the INL revealed a row of large cells with abundant cytoplasm in the middle to outer portion of the INL (Figure 2B, Xie et al, 2018 [Figure 4]). Immunofluorescence (Figure 3A) and immunohistochemical (Figure 3B) studies revealed that these large cells exhibited nuclear and cytoplasmic staining with anti-calbindin antibody (i.e., horizontal cells).…”

Section: Resultsmentioning

confidence: 99%

“…On light microscopy, the horizontal cell cytoplasm appeared to correspond with a discontinuous hyper-reflective band within the hypo-reflective INL (Figure 3). On electron microscopy, the horizontal cell processes in the middle to outer portion of the INL contained numerous mitochondria (Figure 4 and Xie et al, 2018 [Figure 5]). Adjacent bipolar cells also contained abundant mitochondria (Figure 4 and Xie et al, 2018 [Figure 5]).…”

Section: Resultsmentioning

confidence: 99%

“…In order to create a reproducible reflectivity profile, the position of the retinal fundus image was standardized. The position of the eye was maintained with the optic disc exactly in the lower center of the fundus region visualized with the scanning-laser ophthalmoscopy (SLO) module of the Spectralis HRA+OCT (Xie et al, 2018 [Figure 1]), and all OCT scans were acquired in this position. The retina was imaged by SD-OCT along the vertical meridian through the optic nerve head.…”

Section: Methodsmentioning

confidence: 99%

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Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina

Xie

Zhao

Tsai

et al. 2018

Experimental Eye Research

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Spectral domain optical coherence tomography (SD-OCT) is used as a non-invasive tool for retinal morphological assessment in vivo. Information on the correlation of SD-OCT with retinal histology in the porcine retina, a model resembling the human retina, is limited. Herein, we correlated the hypo- and hyper-reflective bands on SD-OCT with histology of the lamellar architecture and cellular constituents of the porcine retina. SD-OCT images were acquired with the Heidelberg Spectralis HRA + OCT. Histological analysis was performed using epoxy resin embedded tissue and transmission electron microscopy. Photomicrographs from the histologic sections were linearly scaled to correct for tissue shrinkage and correlated with SD-OCT images. SD-OCT images correlated well with histomorphometric data. A hyper-reflective band in the mid-to-outer inner nuclear layer correlated with the presence of abundant mitochondria in horizontal cell processes and adjacent bipolar cells. A concentration of cone nuclei corresponded to a relative hypo-reflective band in the outer portion of the outer nuclear layer. The presence of 3 hyper-reflective bands in the outer retina corresponded to: 1) the external limiting membrane; 2) the cone and rod ellipsoid zones; and 3) the interdigitation zone of photoreceptor outer segments/retinal pigment epithelium (RPE) apical cell processes and the RPE. These correlative and normative SD-OCT data may be employed to characterize and assess the in vivo histologic changes in retinal vascular and degenerative diseases and the responses to novel therapeutic interventions in this large animal model.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina

Xie

Zhao

Tsai

et al. 2018

Experimental Eye Research

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“…However, our understanding of factors directly affecting the vasomotor activity of retinal venules is seemingly limited to three studies in pigs, [19][20][21] which resemble human retinal morphology and structure. 22,23 Our previous study found that ET-1 causes marked constriction of porcine retinal venules through ET A receptors (ET A Rs) and extracellular Ca 2+ influx independent of L-type voltage-operated Ca 2+ channels. 20 We have also shown that hyperglycemia, a hallmark of diabetes, augments porcine venular constrictions to ET-1, U46619, and norepinephrine.…”

Section: Introductionmentioning

confidence: 99%

Hyperglycemia Augments Endothelin-1–Induced Constriction of Human Retinal Venules

Chen

Rosa

et al. 2020

Trans. Vis. Sci. Tech.

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Endothelin-1 (ET-1) is a potent vasoactive factor implicated in development of diabetic retinopathy, which is commonly associated with retinal edema and hyperglycemia. Although the vasomotor activity of venules contributes to the regulation of tissue fluid homeostasis, responses of human retinal venules to ET-1 under euglycemia and hyperglycemia remain unknown and the ET-1 receptor subtype corresponding to vasomotor function has not been determined. Herein, we addressed these issues by examining the reactivity of isolated human retinal venules to ET-1, and results from porcine retinal venules were compared. Methods: Retinal tissues were obtained from patients undergoing enucleation. Human and porcine retinal venules were isolated and pressurized to assess diameter changes in response to ET-1 after exposure to 5 mM control glucose or 25 mM high glucose for 2 hours. Results: Both human and porcine retinal venules exposed to control glucose developed similar basal tone and constricted comparably to ET-1 in a concentrationdependent manner. ET-1-induced constrictions of human and porcine retinal venules were abolished by ET A receptor antagonist BQ123. During high glucose exposure, basal tone of human and porcine retinal venules was unaltered but ET-1-induced vasoconstrictions were enhanced. Conclusions: ET-1 elicits comparable constriction of human and porcine retinal venules by activation of ET A receptors. In vitro hyperglycemia augments human and porcine retinal venular responses to ET-1. Translational Relevance: Similarities in vasoconstriction to ET-1 between human and porcine retinal venules support the latter as an effective model of the human retinal microcirculation to help identify vascular targets for the treatment of retinal complications in patients with diabetes.

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Laser-Induced Choroidal Neovascularization in Rats

Zhao

Xie

Hein

et al. 2021

Methods in Molecular Biology

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Data on SD-OCT image acquisition, ultrastructural features, and horizontal tissue shrinkage in the porcine retina

Cited by 5 publications

References 2 publications

Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina

Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina

Hyperglycemia Augments Endothelin-1–Induced Constriction of Human Retinal Venules

Laser-Induced Choroidal Neovascularization in Rats

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