Data on differentially expressed miRNAs in dogs infected with Leishmania infantum

Bragato, Jaqueline Poleto; Melo, Larissa Martins; Venturin, Gabriela Lovizutto; Rebech, Gabriela Torres; Garcia, Leandro Encarnação; Lopes, Flávia L.; Lima, Valéria Marçal Felix de

doi:10.1016/j.dib.2018.01.007

Cited by 4 publications

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“…Given that immunological responses can be regulated by miRNAs [18], we performed microarray for a comparative analysis of miRNA expression in SL of dogs with CanL and control dogs. We observed that miR 21, miR 148a, miR 7 and miR 615 showed increased expression (3.2, 2.3, 2.4 and 2.3 times, respectively), while a decrease in miR 150, miR 125a, miR 125b was observed in CanL (Fig 1A and 1B).…”

Section: Resultsmentioning

confidence: 99%

“…For the infected group, eight dogs naturally infected by L . infantum [18], with at least three clinical signs of the disease, including onychogrifosis, weight loss, ear-tip injuries, periocular lesions, alopecia, skin lesions and lymphadenopathy, and with serological and molecular diagnosis positive for leishmaniasis (S3 Table) were chosen for the study. Infected dogs were further classified in the clinical stage II of the disease, based on blood count results (S1 Table) and biochemical profile (S2 Table) [19].…”

Section: Methodsmentioning

confidence: 99%

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Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes

et al. 2019

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Visceral Leishmaniasis is a chronic zoonosis and, if left untreated, can be fatal. Infected dogs have decreased cellular immunity (Th1) and develop a potent humoral response (Th2), which is not effective for elimination of the protozoan. Immune response can be modulated by microRNAs (miRNAs), however, characterization of miRNAs and their possible regulatory role in the spleen of infected dogs have not been done. We evaluated miRNA expression in splenic leukocytes (SL) from dogs naturally infected with Leishmania infantum and developing leishmaniasis (CanL; n = 8) compared to healthy dogs (n = 4). Microarray analysis showed increased expression of miR 21, miR 148a, miR 7 and miR 615, and downregulation of miR 150, miR 125a and miR 125b. Real-time PCR validated the differential expression of miR 21, miR 148a and miR 615. Further, decrease of miR 21 in SL, by means of transfection with a miR 21 inhibitor, increased the IL-12 cytokine and the T-bet/GATA-3 ratio, and decreased parasite load on SL of dogs with CanL. Taken together, these findings suggest that L. infantum infection alters splenic expression of miRNAs and that miR 21 interferes in the cellular immune response of L. infantum-infected dogs, placing this miRNA as a possible therapeutic target in CanL.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes

et al. 2019

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“…This study was approved by the Committee of Ethics in Animal Experimental Research (COBEA), with the approval of the Institutional Animal Care and Use Committee of UNESP–Universidade Estadual Paulista "Júlio de Mesquita Filho"—Araçatuba—School of Veterinary Medicine—FMVA (process 00978/2016). The generation of the dataset analysed within this manuscript is available in a data descriptor article [17].…”

Section: Methodsmentioning

confidence: 99%

Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis

et al. 2018

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Visceral leishmaniasis (VL) in humans is a chronic and often fatal disease if left untreated. Dogs appear to be the main reservoir host for L. infantum infection, however, in many regions other canids such as jackals, foxes, wolves and other mammals, such as hares or black rats, have been implicated as wild reservoirs. Most dogs cannot form an effective immune response against this infection, and this could be modulated by small non-coding RNAs, called microRNAs, responsible for post-transcriptional control of gene expression. Here, we evaluated the expression of miRNAs in peripheral blood mononuclear cells (PBMC) of symptomatic dogs naturally infected with Leishmania (L.) infantum (n = 10) and compared to those of healthy dogs (n = 5). Microarray analysis revealed that miR-21, miR-424, miR-194 and miR-451 had a 3-fold increase in expression, miR-192, miR-503, and miR-371 had a 2-fold increase in expression, whereas a 2-fold reduction in expression was observed for miR-150 and miR-574. Real-time PCR validated the differential expression of miR-21, miR-150, miR-451, miR-192, miR-194, and miR-371. Parasite load of PBMC was measured by real-time PCR and correlated to the differentially expressed miRNAs, showing a strong positive correlation with expression of miR-194, a regular positive correlation with miR-371 expression, and a moderate negative correlation with miR-150 expression in PBMC. These findings suggest that Leishmania infection interferes with miRNAs expression in PBMC, and their correlation with parasite load may help in the identification of therapeutic targets in Canine Visceral Leishmaniasis (CVL).

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“…The differential expression pattern of miRNAs as well as their relationship with the immune response and parasite load have been recently investigated in PBMCs and splenic leucocytes (SL) of Canine VL (CVL)-infected dogs by L. infantum (Bragato et al, 2018a(Bragato et al, , 2018bMelo et al, 2019). In infected PBMCs, miR-21, miR-194, miR-424 and miR-451 showed a three-fold expression increase, miR-192, miR-371 and miR-503 denoted two-fold increase in their expression, whereas a two-fold decrease in miR expression level was detected for miR-150 and miR-574.…”

Section: Expression Patterns Of Mirnas In Peripheral Blood Mononuclear Cells (Pbmcs) and Macrophagesmentioning

confidence: 99%

Highlighting the interplay of microRNAs from Leishmania parasites and infected-host cells

et al. 2021

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Data on differentially expressed miRNAs in dogs infected with Leishmania infantum

Cited by 4 publications

References 5 publications

Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes

Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes

Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis

Highlighting the interplay of microRNAs from Leishmania parasites and infected-host cells

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