Data of metal and microbial analyses from anaerobic co-digestion of organic and mineral wastes

Abstract

High concentrations of minerals, heavy metals are often found in mineral wastes (MWs) originated from municipal solid waste incineration plants, so as construction/demolition sites. Such by-products (minerals) often have buffering capacity. The current work provides analysis of total and soluble (dissolved) metal concentrations released by four different MWs (a. cement-based waste, b. incineration (bottom), c. fly and d. boiler ash) supplemented to anaerobic reactors of organic waste at 37 °C. The reactors (continuous stirred tank reactor (CSTR)) were ran for 75 days at hydrolytic retention time of 20 days. Genomic DNA extraction, and qPCR and Illumina HiSeq (16S V4) analyses were conducted to investigate microbial community population and composition in anaerobic digestate samples collected from these reactors. Output data from Illumina sequencing analysis were FastQ files analysed using the QIIME2 pipeline to produce a feature table listing the frequency of each assigned microbial taxa per samples. Additional study was conducted on the microbial data to visualise variations in microbial communities using the STAMP software and phyloseq R package. Detailed interpretation and discussion of the results can be found in the related research article [1].

Low-Temperature Pretreatment of Organic Feedstocks with Selected Mineral Wastes Sustains Anaerobic Digestion Stability through Trace Metal Release

“…Total metal concentrations in the SOW (Table ) and MW (Table SI-1) were measured by an aqua regia digestion method, as described previously . Dissolved concentrations of metals in the inoculum (Table ) and anaerobic digestate samples were measured from supernatants (obtained after centrifugation at 3392 g for 30 min (Sigma centrifuge, U.K.)) filtered through sterile syringe filters (0.2 μm pore size) by inductively coupled plasma optical emission spectroscopy (ICP-OES) .…”

“…Total metal concentrations in the SOW (Table ) and MW (Table SI-1) were measured by an aqua regia digestion method, as described previously . Dissolved concentrations of metals in the inoculum (Table ) and anaerobic digestate samples were measured from supernatants (obtained after centrifugation at 3392 g for 30 min (Sigma centrifuge, U.K.)) filtered through sterile syringe filters (0.2 μm pore size) by inductively coupled plasma optical emission spectroscopy (ICP-OES) . As controls, blank samples were prepared following the same sample-preparation and analytical processes as with the real samples.…”

“…The four types of MW (Table 2) used in this study were collected from two different industrial plants, namely, (i) IBA, FA, and BA from a domestic Energy-from-Waste incineration power plant, Teesside, U.K. (household food and garden wastes are burnt at ≈ 1000 °C); (ii) CBW (with nominal particle sizes of 10 and 1 mm) collected from a construction demolition waste recycling site (Thompsons), Newcastle upon Tyne, U.K. For the homogenization of the 1) and MW (Table SI-1) were measured by an aqua regia digestion method, as described previously. 25 Dissolved concentrations of metals in the inoculum (Table 1) and anaerobic digestate samples were measured from supernatants (obtained after centrifugation at 3392g for 30 min (Sigma centrifuge, U.K.)) filtered through sterile syringe filters (0.2 μm pore size) by inductively coupled plasma optical emission spectroscopy (ICP-OES). 25 As controls, blank samples were prepared following the same sample-preparation and analytical processes as with the real samples.…”

Sewage treatment at 4 °C in anaerobic upflow reactors with and without a membrane – performance, function and microbial diversity

“…Detailed procedure for microbial analysis including DNA extraction, qPCR and Illumina HiSeq sequencing analysis was similar to that previously described in Shamurad et al, (2019Shamurad et al, ( , 2019b). [29][30][31] DNA extraction: Biomass samples were obtained from the pellets formed after centrifugation of a mixture of mixed liquor and biofilm samples (3 minutes, 14 000 rpm of total vol. of 1 ml).…”

“…The 16s rRNA gene data was processed via 'Quantitative Insights Into Microbial Ecology' (QIIME 1.9.1 pipeline (Caporaso et al, 2010) 37 ) as described in Shamurad et al, (2019). [29][30][31] In QIIME2 a table of representative sequences (taxa) in the samples was produced. Then, a feature table containing the frequencies of each taxon per samples was produced by comparing the representative sequences with the taxon in the SILVA119 reference database.…”