Data of first de-novo transcriptome assembly of a non-model species, hawksbill sea turtle, Eretmochelys imbricate , nesting of the Colombian Caribean

Abstract

The hawksbill sea turtle, Eretmochelys imbricata, is an endangered species of the Caribbean Colombian coast due to anthropic and natural factors that have decreased their population levels. Little is known about the genes that are involved in their immune system, sex determination, aging and others important functions. The data generated represents RNA sequencing and the first de-novo assembly of transcripts expressed in the blood of the hawksbill sea turtle. The raw FASTQ files were deposited in the NCBI SRA database with accession number SRX2653641. A total of 5.7 Gb raw sequence data were obtained, corresponding to 47,555,108 raw reads. Trinity was used to perform a first de-novo assembly, and we were able to identify 47,586 transcripts of the female hawksbill turtle transcriptome with an N50 of 1100 bp. The obtained transcriptome data will be useful for further studies of the physiology, biochemistry and evolution in this species.

Detection of high heteroplasmy in complete loggerhead and hawksbill sea turtles mitochondrial genomes using RNAseq

“…Currently, the most reliable method to measure the degree of heteroplasmy is the Next Generation Sequencing technology (NGS) (Li et al 2010). This method has been used in recent years (Van Der Walt et al 2012;Dölle et al 2016;Rygiel et al 2016;Hernández-Fernández 2017;Hernández-Fernández et al 2017;Tikochinski et al 2020), as it provides more accurate data and produces millions of DNA readings in a single run at a low cost. Unlike Sanger sequencing, which only distinguishes two haplotypes in an individual regardless of their relative frequencies, NGS sequencing allows mutations in the mitochondrial genome to be identified, heteroplasmy levels to be measured, and detailed molecular diagnoses of mitochondrial diseases to be made (Li et al 2010).…”