Data in support of alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects in vascular smooth muscle cells

Abstract

The data provides information in support of the research article Moraes et al., Atherosclerosis 243(2) (2015) 477–485 [1]. Here we provide data behind the mechanisms involved in Angiotensin II (Ang II) effects on vascular smooth muscle cells (VSMC). Ang II-induced VSMC ROS production is modulated by alpha1beta1 integrin. Ang II also stimulates ROS production in VSMC via p47phox, a NOX2 subunit. Furthermore, Ang II effect on VSMC migration was also inhibited by NOX2 inhibitor. We showed that obtustatin, alpha1beta1 integrin blocker, inhibited Ang II effect on p47phox activation. Ang II effect on ROS production is also PI3K dependent. Finally we showed that NOX1 and Integrin-Linked-Kinase (ILK) are crucial to NOX2 activation. The research provides information about the sequential events of NOX1/alpha1beta1 integrin/ILK/NOX2 in Ang II effects on VSMC.

Metalloproteases Affecting Blood Coagulation, Fibrinolysis and Platelet Aggregation from Snake Venoms: Definition and Nomenclature of Interaction Sites

“…In addition, they also exhibit uniquely exclusive effects on smooth muscle cells [191,192], fibroblast-like cells [193,194], chondrocytes [195], osteoblasts [196], and neuronal progenitors [197]. Recent studies using obtustatin has shown that α 1 β 1 integrin and integrin-linked kinase modulate angiotensin II effects in vascular smooth muscle cells and thus, are potential targets to the development of more effective therapeutic interventions in cardiovascular diseases [198,199]. Thus, D and C domains selectively target specific integrins and play critical role in our understanding of cell biology.…”

Effects of Lonomia obliqua Venom on Vascular Smooth Muscle Cells: Contribution of NADPH Oxidase-Derived Reactive Oxygen Species

“…Increases in ROS production, which are mainly intracellular, have been shown to modulate different VSMC functions [ 18 , 19 ]. Thus, we investigated whether LOCBE could induce oxidative stress in VSMC.…”

The role of the cell-matrix interface in aging and its interaction with the renin-angiotensin system in the aged vasculature

“…Ang II controls vascular cell production of ROS through various Noxs, including Nox-1 and Nox-2 (Nguyen Dinh Cat et al, 2013). Interestingly, studies by Moers and collaborators (Moraes et al, 2015, 2016) have provided evidence that Ang II produces ROS in the VSMC in two stages, the second of which is mediated by the α1β1 integrin signaling. Based on their data, the authors propose that Ang II signaling briefly stimulates Nox-1 activity leading to the early phase of ROS production.…”

“…Based on their data, the authors propose that Ang II signaling briefly stimulates Nox-1 activity leading to the early phase of ROS production. This is followed by activation of the α1β1 integrin/ILK signaling that, in turn, induces the second phase of ROS production via Nox-2 activation (Moraes et al, 2015, 2016).…”