Data from Tumor Paint: A Chlorotoxin:Cy5.5 Bioconjugate for Intraoperative Visualization of Cancer Foci

Abstract

<div>Abstract<p>Toward the goal of developing an optical imaging contrast agent that will enable surgeons to intraoperatively distinguish cancer foci from adjacent normal tissue, we developed a chlorotoxin:Cy5.5 (CTX:Cy5.5) bioconjugate that emits near-IR fluorescent signal. The probe delineates malignant glioma, medulloblastoma, prostate cancer, intestinal cancer, and sarcoma from adjacent non-neoplastic tissue in mouse models. Metastatic cancer foci as small as a few hundred cells were detected in lymph channels. Specific binding to cancer cells is facilitated by matrix metalloproteinase-2 (MMP-2) as evidenced by reduction of CTX:Cy5.5 binding <i>in vitro</i> and <i>in vivo</i> by a pharmacologic blocker of MMP-2 and induction of CTX:Cy5.5 binding in MCF-7 cells following transfection with a plasmid encoding MMP-2. Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies. [Cancer Res 2007;67(14):6882–8]</p></div>

Investigation of Cytotoxic and Antiproliferative Effects of Mesobuthus gibbosus (Scorpiones: Buthidae) Crude Scorpion Venom on Cancer Cell Lines

“…Klorotoksin ile yapılan çalışmalar, bu toksinin glioma kanser tedavisinde kullanılabileceğini göstermiştir. Yapılan çalışmalar akrep zehrinden izole edilen klorotoksinin (CLTx) glioma hücrelerini spesifik olarak tanıdığını ve glioma hücrelerine spesifik olan bir klor iyon kanalını eksprese ettiğini gösterilmiştir (18)(19)(20)(21).…”