Das Lymphgewebe der weißen maus bei saurer und basischer Ernährung

Hoepke, Hermann; Hempfing, Werner; Desaga, H.

doi:10.1007/bf02118848

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…Our observations with immunofluorescence analysis confirm earlier reports that lymphatic capillaries are present in mouse thymic capsule (Hammar 1936;Hoepke and Peter 1936). Lymphatic vessels have been demonstrated in the thymic medulla, but not beyond the corticomedullary junction (Hwang et al 1974).…”

Section: Discussionsupporting

confidence: 94%

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Distribution of lymphatic vessels in mouse thymus: immunofluorescence analysis

et al. 2006

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Thymic blood and lymphatic vessels in humans and laboratory animals have been investigated in morphological studies. However, occasionally a clear distinction between blood vessels and lymphatic vessels cannot be made from morphological characteristics of the vasculature. To visualize thymic lymphatics in normal adult BALB/c mice, we used antibodies against specific markers of lymphatic endothelial cells. Expression of vascular endothelial growth factor receptor-3 (VEGFR-3) was detected throughout the thymus, i.e., the capsule, cortex, and medulla. Most thymic lymphatics were present in capillaries of ~20 mum in caliber. The plexuses of lymphatic capillaries were occasionally detectable. Lymphatic vessels were frequently adjacent to CD31-positive blood vessels, and some lymphatic vessels were seen in the immediate vicinity of or within the perivascular spaces around postcapillary venules. The identity of VEGFR-3-positive vessels as lymphatics was further confirmed by staining with additional markers: LYVE-1, Prox-1, neuropilin-2, and secondary lymphoid tissue chemokine (SLC). The distributions of LYVE-1 were similar to those of VEGFR-3. Most lymphatic vessels were also identified by Prox-1. Neuropilin-2 was restricted to lymphatic vessels in the thymus. The most abundant expression of SLC in the thymus was in medullar epithelial cells; SLC was also expressed in lymphatic vessels and blood vessels. Thus, lymphatic endothelium in mouse thymus was characterized by positive staining with antibodies to VEGFR-3, LYVE-1, Prox-1, neuropilin-2, or SLC, but not with an antibody to CD31. Our results suggest the presence of lymphatic capillary networks throughout the thymus.

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Section: Discussionsupporting

confidence: 94%

“…Earlier investigations have demonstrated the presence of lymphatic vessels in the capsule and the interlobular connective tissue (Hammar 1936;Hoepke and Peter 1936). Lymphatic vessels are reported to be accompanied by blood vessels in mouse thymus (Dunn 1954).…”

Section: Introductionmentioning

confidence: 99%

Distribution of lymphatic vessels in mouse thymus: immunofluorescence analysis

et al. 2006

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“…The presence of lymphatic vessels in the capsule and interlobular connective tissue of the thymus was made known in laboratory animals and humans by early investigators (Bargmann, 1943;Hammar, 1936;Hoepke and Peter, 1936;Hoepke and Spanier, 1939). Using a dye injection method, Dunn (1954) indicated lymphatic vessels as irregularly widened spaces, which accompanied blood vessels in the mouse thymus.…”

Section: Introduction: Historical Backgroundmentioning

confidence: 99%

Thymic microvascular system

Kato

1997

Microsc. Res. Tech.

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Morphological studies of the microcirculatory system in the thymus were reviewed in regards to methodology and structural organization of blood and lymphatic vessels. The blood capillaries and postcapillary venules (PCVs) in the thymus are characterized by a double‐walled structure. These vessels are surrounded more or less by perivascular spaces (PVSs) containing many lymphocytes. This space is delimited on the one side by abluminal surface of the vascular endothelium and on the other side by cytoplasmic processes of epithelial reticular cells. There are interruptions or gaps on the outer epithelial reticular layer. The lymphatic vessels can be distinguished histochemically from blood vessels based on strong 5′‐nucleotidase (5′‐Nase) activity. The 5′‐Nase‐positive lymphatic vessels were seen predominantly in the capsule and interlobular connective tissue but sometimes in the immediate vicinity of the PVS around the PCV, when a discrete opening in the lymphatic wall next to the PVS was found. Thus, it may be regarded as an initial part of lymphatics closely associated with the PVS, suggesting a possible route for lymphocyte efflux into the lymphatic vessel from the PVS. The endothelial cells of lymphatic vessels as well as PCVs are often infiltrated by lymphocytes, particularly more heavily during acute involution of the thymus. These images represent the migration of lymphocytes into the blood or lymphatic microcirculation. Microsc. Res. Tech. 38:287–299, 1997. © 1997 Wiley‐Liss, Inc.

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“…Works investigating weight decrease of the thymus provoked by hunger, and especially vitamin deficiency, are well known (J a n s o n, 1909; H a m m a r, 1941; Bargmann, 1943). The kind of food may also have a considerable influence on the thymus (H o e p k e & Peter, 1936).…”

Section: 7%mentioning

confidence: 99%