Das isoliert perfundierte Hinterbein der Ratte als Modell zur Untersuchung des Muskelstoffwechsels

Abstract

The Isolated Perfused Hindquarter of the Rat as a Model for Studying Muscle MetabolismSummary. 1. A detailed description is given of the perfusion technique of the rat hindquarter with a semisynthetie medium. 2. The concentrations of creatine phosphate, ATP and ADP in the muscle tissue after 60--180 min of perfusion were identical with those measured in vivo.3. The glucose uptake of the resting hindquarter of male fed rats was 175.2 tzMol per 30 g/hr 63% of the oxygen consumption accounted for glucose oxidation. No lactate was released to the medium during the first hour of perfusion.4. Insulin (1 mU/ml) caused a 4-fold increase of the glucose uptake, a 7-fold accretion of the muscle glycogen and a 2~-fold increase of the supposed oxydized part of glucose.5. The perfusion of the isolated hindquarter of the rat proved as an appropriate model for the study of skeletal muscle metabolism.Key words: Perfusion of the hindquarter of the rat --Skeletal muscle --Carbohydrate metabolism. Zusammen/assung. 1. Es wird eine ausffihrliche Beschreibung dcr Methode derHinterbeinperfusion mit einem semisynthetischen Medium an der Ratte mitgeteilt.2. Des Phosphorylierungspotential des fiber 60 bis 180 rain perfundiertenMuskelgewebes war mit dem in rive gemessenen identisch. Des elektronenoptische Bild zeigte im Verlauf der Perfusion fiber 60 rain keine VerEnderung der Strukturen. 3. Die Glueoseaufnahme des ruhenden Muskels niehtgehungerter m~nnlieher Ratten betrug 175,2 fzMol/30 g/St& 63% des aufgenommenen Sauerstoffes wurden ffir die Oxydation der Glucose verbraucht. Eine Lactatabgabe an des Medium war w~hrend der Perfusion fiber 60 rain nicht zu beobachten.4. Insulin (1 mE/ml) bewirkte einen 4fachen Anstieg der Glucoseaufnahme, eine Glykogenzun~hme um des Siebenfaehe sowie eine Steigerung des vermutlich oxydierten Glucoseanteiles um des 2~faehe. Die Glycerinabgabe wurde um die ItElfte reduziert, ebenso die Konzentration yon Alanin im Medium.

Effects of buformin on the metabolism of the isolated haemoglobin-free perfused hindlimb of normal rats

“…Similar results were reported with the perfused rat heart [15] and in in vivo experiments [11]. In order to investigate a direct action of buformin on glucose utilization in skeletal muscle, the isolated perfused hindlimb of the rat was used as a suitable model for metabolic studies [13,14]. In this study we report the effect of low and high doses of buformin, administered orally for one or several days, on the metabolism of the perfused resting hindlimb of normal rats.…”

“…The perfusion lasted for 1 h and started 2.5 h after the last drug administration. The perfusion technique, apparatus, synthetic medium and analytical * Supported by a grant of the Deutsche Forschungsgemeinschaft methods used have been reported in detail elsewhere [13,14].…”

Pyrimidine Nucleotide Biosynthesis and Turnover in Rat Skeletal Muscle and Liver

“…Insulin (1 I.U.jl50 ml) stimulated the uptake 3-fold to about 5.1 pmol x min-'. The higher stimulation of glucose uptake reported by others [15,35,36] might be attributed to higher glucose concentrations in the perfusate and to the intact innervation [15,35].…”

“…It is also a most useful indicator for the finctional integrity of the perfused hindquarter [15,35]. Generally, a flow rate through the hindquarter of more than 8 ml x min-' is held necessary [15,35 -371 using an erythrocyte-containing perfusate.…”

“…As the perfusate was a hemoglobin-free Krebs/ Henseleit/bicarbonate buffer, the flow rate was of great importance to assure a sufficient oxygen supply. It is also a most useful indicator for the finctional integrity of the perfused hindquarter [15,35]. Gene-rally, a flow rate through the hindquarter of more than 8 ml x min-' is held necessary [15,35 -371 using an erythrocyte-containing perfusate.…”