Das dynamische Verhalten der Innenohrflüssigkeiten*

Giebel, W.

doi:10.1055/s-2007-1008612

Cited by 11 publications

(4 citation statements)

References 10 publications

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“…Although several studies have investigated the dispersal of macromolecular marker substances, such as trypan blue [3, 75], fluorescein [22, 23], thorotrast [4] or peroxidase [37], between the endolymphatic and perilymphatic spaces of the inner ear in vivo, these techniques were not adequate to investigate permeation through the highly water-specific AQP channels. In contrast, radioactively labelled water (tritiated water; THO) has a similar molecular structure as the water molecule (H 2 O) with the exception of one hydrogen (H) that is substituted with tritium ( 3 H).…”

Section: Methodsmentioning

confidence: 99%

Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Eckhard

Müller

Salt

et al. 2014

Pflugers Arch - Eur J Physiol

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The cochlear duct epithelium (CDE) constitutes a tight barrier that effectively separates the inner ear fluids, endolymph and perilymph, thereby maintaining distinct ionic and osmotic gradients that are essential for auditory function. However, in vivo experiments have demonstrated that the CDE allows for rapid water exchange between fluid compartments. The molecular mechanism governing water permeation across the CDE remains elusive. We computationally determined the diffusional (PD) and osmotic (Pf) water permeability coefficients for the mammalian CDE based on in silico simulations of cochlear water dynamics integrating previously derived in vivo experimental data on fluid flow with expression sites of molecular water channels (aquaporins, AQPs). The PD of the entire CDE (PD = 8.18 × 10−5 cm s−1) and its individual partitions including Reissner's membrane (PD = 12.06 × 10−5 cm s−1) and the organ of Corti (PD = 10.2 × 10−5 cm s−1) were similar to other epithelia with AQP-facilitated water permeation. The Pf of the CDE (Pf = 6.15 × 10−4 cm s−1) was also in the range of other epithelia while an exceptionally high Pf was determined for an epithelial subdomain of outer sulcus cells in the cochlear apex co-expressing AQP4 and AQP5 (OSCs; Pf = 156.90 × 10−3 cm s−1). The Pf/PD ratios of the CDE (Pf/PD = 7.52) and OSCs (Pf/PD = 242.02) indicate an aqueous pore-facilitated water exchange and reveal a high-transfer region or “water shunt” in the cochlear apex. This “water shunt” explains experimentally determined phenomena of endolymphatic longitudinal flow towards the cochlear apex. The water permeability coefficients of the CDE emphasise the physiological and pathophysiological relevance of water dynamics in the cochlea in particular for endolymphatic hydrops and Ménière's disease.

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Section: Methodsmentioning

confidence: 99%

Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Eckhard

Müller

Salt

et al. 2014

Pflugers Arch - Eur J Physiol

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“…Giebel [11] führte während seiner experimentellen Untersuchungen zum dynamischen Verhalten der Innenohrflüssigkeiten unter anderem die intravenöse Applikation von Fluorescein durch.…”

Section: Schlüsselwörterunclassified

“…optisch nicht nachweisbare Mengen Perilymphe können durch den Fluoreszenzeffekt entdeckt werden. Die Anwendung des Fluorescein als Substanz in der Otologie erfolgte bisher vorwiegend experimentell [1,11]. Klinisch-otologische Anwendungen, z.…”

Section: Diskussionunclassified

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Rundfensterruptur - Nachweis mittels Fluoreszenzendoskopie

Kleemann¹,

Nofz²,

Plank³

et al. 2001

HNO

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Rupture of the round window membrane as a special cause of inner ear deafness is widely accepted after changing pressure levels, e.g. in diving. However, even without a barotrauma before, the spontaneous rupture of the round window membrane is suspected occasionally in patients with sudden hearing loss and/or vertigo and tinnitus. To carry through the tympanotomy is decided by ENT surgeons often in cases of progressive hearing loss despite infusion therapy. Perilymph fistulas have been detected relatively seldom, compared to the number of reported operations by several authors. However, covering the round niche with connective tissue leads to the improvement of symptoms sometimes even in cases without microscopical evidence of fistula. Within the last 3 years 14 patients suffering sudden hearing loss of one ear underwent tympanotomy in our department. Of these patients 8 reached restitution of the hearing ability. Especially 2 patients with sudden deafness caused by spontaneous rupture of the round window membrane are reported in the following article. Perilymph fistulas were detected in these cases by IV-application of fluorescein and fluorescence endoscopy of the middle ear. Both patients obtained a normal hearing curve within 1 week after surgical intervention and obliteration of the round niche.

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Composition, formation and flow of intralabyrinthine fluids

Giebel

1996

Intracranial and Intralabyrinthine Fluids

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Das dynamische Verhalten der Innenohrflüssigkeiten*

Abstract: Eine Beteiligung der Innenohrflussigkeiten an der Stoffwechselphysiologie der Sinneszellen des Cortischen Organs war wegen der moglichen Störung dieser Funktion bei bestimmten Erkrankungen des Innenohres bereits Thema zahireicher Untersuchungen.

Cited by 11 publications

References 10 publications

Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph–endolymph barrier

Rundfensterruptur - Nachweis mittels Fluoreszenzendoskopie

Composition, formation and flow of intralabyrinthine fluids

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