Das Colitis ulcerosa-assoziierte kolorektale Karzinom - DNS-Ploidie als Indikator einer drohenden malignen Transformation? -

Schimmelpenning, H.; Habermann, Jens K.; Krüger, S.; Uj, Roblick; Stange, Eduard F.; Ludwig, D.; Kujath, P.; Broll, R.; Auer, Gert; Hp, Bruch

doi:10.1055/s-2000-10063

Cited by 1 publication

(3 citation statements)

References 14 publications

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“…According to Fozard et al, 1986, 42% of UC are aneuploid with higher rate of DNA aneuploidy in dysplastic tissues (21%) compared with non-dysplastic tissues (15%). Schimmelpenning et al, (2000) detected aneuploidy in 25% of UC cases and all developed adenocarcinoma within seven years follow up.…”

Section: Discussionmentioning

confidence: 94%

“…Colorectal cancer (CRC) is one of most common malignancy worldwide. Aneuploidy is an inevitable result of chromosomal instability and can be detected quantitively using automated image-based DNA cytometry (Danielsen et al, 2016) (Schimmelpenning et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Detection of DNA aneuploidy in mucosal biopsies may be more reliable marker for subsequent malignant transformation of the colorectal mucosa (Beaugerie and Itzkowitz, 2015). Aneuploidy as well occurs as an early genetic alteration preceding morphologic changes of neoplasia, it is not confined to dysplastic epithelium only, DNA aneuploidy in flat mucosa may constitute an additional marker in the identification of patients at increased cancer risk who could benefit from a closer surveillance (Schimmelpenning et al, 2000).…”

Section: O M P a R A T I V E S T U D Y O F D N A P L O I D Y A N D B ...mentioning

confidence: 99%

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Comparative Study of DNA ploidy and BRAF Immunohistochemistry between Colonic Adenocarcinoma and Inflammatory Colonic Lesions

Khalil

Hammam

Anis

et al. 2023

Asian Pac J Cancer Prev

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Objectives: To evaluate DNA ploidy and S-phase fraction (SPF) in non-Lynch colonic adenocarcinoma, ulcerative colitis (UC), Crohn disease (CD) which are known as risk factors, and colitis. We correlated ploidy and SPF with tumor grading, staging and BRAF expression. Methods: All studied adenocarcinomas have intact mismatch repair genes as proved by immunohistochemistry. All were assessed for ploidy by automated image-based DNA cytometry and histograms were drawn. Immunostaining by anti- BRAF V600E was performed. Diagnostic laparoscopy (DL) was done as a preliminary step for staging GI cancers. Results: there is significant difference in DNA ploidy between groups; 77.5% and 17.5% of aneuploid cases are adenocarcinoma and UC. Groups are compared in terms of 2C, 4C, above 4C DNA content and SPF and significant difference is principally found between adenocarcinoma group and others. In adenocarcinomas, DNA ploidy is significantly correlated with tumor staging and grading. Regarding BRAF expression, there is significant difference between groups; all adenocarcinomas, 83.33% of UC were positive, while all cases of colitis, bilharzial colitis, CD were negative. There is significant relation between BRAF and SPF among all diploid cases including adenocarcinoma, and among non-neoplastic diploid cases. There is direct significant relation between BRAF intensity and adenocarcinoma staging. There is no significant difference between BRAF and ploidy among UC cases, although 75% of aneuploid UC are positive. DL helps in GI cancer staging. Routine laparoscopy before laparotomy, especially in cancers which have equivocal operability helps to avoid unnecessary laparotomies. Conclusion: Based on significant difference in ploidy between adenocarcinoma and UC and between SPF and ploidy, assessment of ploidy by DNA cytometry for UC and other colitis could therefore predict impending malignant transformation before development of colonic dysplasia. Also measuring SPF in adenocarcinoma helps to select patients who could greatly benefit from chemotherapy. DL has vital role in staging GI cancers.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: O M P a R A T I V E S T U D Y O F D N A P L O I D Y A N D B ...mentioning

confidence: 99%

See 1 more Smart Citation

Comparative Study of DNA ploidy and BRAF Immunohistochemistry between Colonic Adenocarcinoma and Inflammatory Colonic Lesions

Khalil

Hammam

Anis

et al. 2023

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

show abstract

Das Colitis ulcerosa-assoziierte kolorektale Karzinom - DNS-Ploidie als Indikator einer drohenden malignen Transformation? -

Cited by 1 publication

References 14 publications

Comparative Study of DNA ploidy and BRAF Immunohistochemistry between Colonic Adenocarcinoma and Inflammatory Colonic Lesions

Comparative Study of DNA ploidy and BRAF Immunohistochemistry between Colonic Adenocarcinoma and Inflammatory Colonic Lesions

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