Dark tea extracts: Chemical constituents and modulatory effect on gastrointestinal function

Gong, Zhiping; Ouyang, Jian; Wu, Xin; Zhou, Fang; Lu, Dan-Ming; Zhao, Chen; Liu, Chunfang; Zhu, Wan fang; Zhang, Jing-chi; Li, Neng-xin; Miao, Feng; Song, Yuxin; Li, Yilong; Wang, Qiye; Lin, Haiyan; Zeng, Xin; Cai, Shumin; Huang, Jianan; Liu, Zhonghua; Zhu, Mingzhi

doi:10.1016/j.biopha.2020.110514

Cited by 51 publications

(38 citation statements)

References 31 publications

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“…Dietary supplementation with 500 mg/kg of GA improved the bacterial diversity, inhibited the growth of Escherichia-Shigella and Clostridium_sensu_stricto_1, and enhanced Lactobacillus and Faecalibaculum, especially 1 day after transportation. Our results were further verified with LEfSe analysis, which also found that the TS group was associated with enrichment of Proteobacteria, Escherichia-Shigella, and E. coli, while Lactobacillus, L. murinus, and L. reuteri dominated the GA treatment (86,87). Spearman's correlation analysis revealed the symbiotic relationship between bacteria.…”

Section: Discussionsupporting

confidence: 76%

Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome–Metabolomics Analysis

et al. 2022

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Early-life exposure to environmental stress disrupts the gut barrier and leads to inflammatory responses and changes in gut microbiota composition. Gallic acid (GA), a natural plant polyphenol, has received significant interest for its antioxidant, anti-inflammatory, and antimicrobial properties that support the maintenance of intestinal health. To assess whether dietary supplementation of GA alleviates environmental stress, a total of 19 puppies were randomly allocated to the following three dietary treatments for 2 weeks: 1) basal diet (control (CON)); 2) basal diet + transportation (TS); and 3) basal diet with the addition of 500 mg/kg of GA + transportation (TS+GA). After a 1-week supplementation period, puppies in the TS and TS+GA groups were transported from a stressful environment to another livable location, and puppies in the CON group were then left in the stressful environment. Results indicated that GA markedly reduced the diarrhea rate in puppies throughout the trial period and caused a moderate decline of serum cortisol and HSP-70 levels after transportation. Also, GA alleviated the oxidative stress and inflammatory response caused by multiple environmental stressors. Meanwhile, puppies fed GA had a higher abundance of fecal Firmicutes and Lactobacillus and lower Proteobacteria, Escherichia–Shigella, and Clostridium_sensu_stricto_1 after transportation. As a result, the TS+GA group had the highest total short-chain fatty acids and acetic acid. Also, the fecal and serum metabolomics analyses revealed that GA markedly reversed the abnormalities of amino acid metabolism, lipid metabolism, carbohydrate metabolism, and nucleotide metabolism caused by stresses. Finally, Spearman’s correlation analysis was carried out to explore the comprehensive microbiota and metabolite relationships. Overall, dietary supplementation of GA alleviates oxidative stress and inflammatory response in stressed puppies by causing beneficial shifts on gut microbiota and metabolites that may support gut and host health.

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Section: Discussionsupporting

confidence: 76%

Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome–Metabolomics Analysis

et al. 2022

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“…Polyphenols are the primary functional ingredient in tea and play a role in scavenging oxygen free radicals and changing gut flora (34). The content of polyphenol and catechins in our study was higher than that of reported content in various dark tea water extract with polyphenol content that ranged from 11.61 to 18.22% and catechin content from 4.81 to 6.83% (35). EGCG, as the most abundant catechin presented in tea, has been widely accepted for its lipid-lowering effects in animal model and clinical research (36,37).…”

Section: Discussioncontrasting

confidence: 71%

Instant Dark Tea Alleviates Hyperlipidaemia in High-Fat Diet-Fed Rat: From Molecular Evidence to Redox Balance and Beyond

Qin

Yuanjie³

et al. 2022

Front. Nutr.

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Instant dark tea (IDT) is a new product gaining increasing attention because it is convenient and can endow significant health benefit to consumers, which is partially attributed to its high concentration of functional ingredients. However, the molecular mechanism underlying its regulatory effect on hyperlipidaemia is rarely studied. In this study, we performed omics and molecular verification in high-fat diet (HFD)-fed rat, aiming to reveal the mechanism and provide molecular evidence. The results showed that the major bioactive components in IDT were 237.9 mg/g total polysaccharides, 336.6 mg/g total polyphenols, and 46.9 mg/g EGCG. Rats fed with IDT (0.27–0.54 g/kg for 12 weeks) significantly reduced the body weight and TC, TG, LDL-C, blood glucose, and MDA and induced the level of serum HDL-C and also the levels of liver SOD, CAT, GSH-Px, and Nrf2, compared to HFD group. For molecular mechanism study, HIDT feeding had significant impact on the gene expressions of biomarkers in lipogenesis (FABP, CD36, SCD1, Cyp4a1, and Kcnn2), lipid oxidation (PPARγ), and glucose glycolysis (Gck and ENO2) in liver tissue. Moreover, gut microbiome study found that rats fed with IDT dramatically modified the gut microbial species at the family level, such as suppressing the increase abundance of Proteobacteria and Firmicutes induced by HFD. HIDT significantly boosted the relative composition of beneficial bacterium Akkermansia and Rikenellaceae_RC9_gut_group and decreased the relative abundance of the harmful bacterium Ruminococcaceae_UCG-005 and Ruminiclostridium_9, compared to HFD (p < 0.01). Correlation analysis between microbiome and animal indicators found that seven genera including Akkermansia, Clostridiales, Lachnospiraceae, Lachnospiraceae_UCG-010, Ruminiclostridium_9, Ruminococaceae-UCG-005, and Ruminocuccus_1 were found as potential biomarkers that were strongly correlated with oxidative stress and metabolism genes. For instance, Ruminococcaceae_UCG-005 was significantly correlated with body weight, TG, HDL-C, Nfr2, FABP3, SCD1, Cyp4a1, and Kcnn2. Collectively, the above data obtained in this study had provided the primary molecular evidence for the molecular mechanism and brought in novel insights based on omics for the regulatory effect of IDT on hyperlipidaemia.

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“…Each mouse was given a daily dose of LPT extract (tea water extracts frozen into dry powder) (mg kg −1 of body weight) = 166.7 × 20 × tea water extractions rate. The recommended daily dose is that which would be consumed if an adult with a 60 kg body weight drank approximately 10 g tea per day, which corresponds to 166.7 mg kg −1 of body weight for an adult; 20 times this amount is the recommended dose for mice; 14 the extraction rates of 1 Y tea and 10 Y tea are 29.21% and 24.69%, respectively. The animal protocols were approved by the Ethical Committee of Hunan Agriculture University (Approval no.…”

Section: Methodsmentioning

confidence: 99%

Untargeted metabolomic and lipid metabolism-related gene expression analyses of the effects and mechanism of aged Liupao tea treatment in HFD-induced obese mice

Zhang

et al. 2021

RSC Adv.

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Dark tea extracts: Chemical constituents and modulatory effect on gastrointestinal function

Cited by 51 publications

References 31 publications

Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome–Metabolomics Analysis

Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome–Metabolomics Analysis

Instant Dark Tea Alleviates Hyperlipidaemia in High-Fat Diet-Fed Rat: From Molecular Evidence to Redox Balance and Beyond

Untargeted metabolomic and lipid metabolism-related gene expression analyses of the effects and mechanism of aged Liupao tea treatment in HFD-induced obese mice

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