Dark nanodiscs as a model membrane for evaluating membrane protein thermostability by differential scanning fluorimetry
Jazlyn A. Selvasingh,
Eli Fritz McDonald,
Jacob R. Mckinney
et al.
Abstract:Measuring protein thermostability provides valuable information on the biophysical rules that govern structure-energy relationships of proteins. However, such measurements remain a challenge for membrane proteins. Here, we introduce a new experimental system to evaluate membrane protein thermostability. This system leverages a recently-developed non-fluorescent membrane scaffold protein (MSP) to reconstitute proteins into nanodiscs and is coupled with a nano-format of differential scanning fluorimetry (nanoDSF… Show more
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