Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles

Abstract

We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample with a localization accuracy independent of the particle position. From our measurements we reconstruct the trajectories of the particles and derive their 3D diffusion coefficient. Our results pave the way for quantitative studies of the motion of single nanoparticle in complex media.

Fish embryo multimodal imaging by laser Doppler digital holography

“…All the driving signals are synchronized by a common 10 MHz clock. Figure 2 illustrates our reconstruction procedure [4,5] with non-Doppler shifted images. We have considered here four phase holograms: H = (I 0 − I 2 ) + j(I 1 − I 3 ) (see Fig.…”

Laser Doppler holographic microscopy in transmission: application to fish embryo imaging

“…The reconstruction procedure is similar to the one used in previous works [50,51]. To illustrate reconstruction, let us consider 4 phase detection of the ballistic peak signal (no Doppler shift).…”

Comparative review of interferometric detection of plasmonic nanoparticles

“…Another study using digital holography showed that using dark field excitation could enable visualization of gold nanoparticles without the need for photothermal excitation [49]. Here the nanoparticles were excited with darkfield illumination and the scattered light was imaged with on axis holography.…”