Daptomycin Susceptibility in Gram Positive Cocci Isolated from Intensive Care Units

Şamlıoğlu, Pınar; Ece, Gülfem; Atalay, Sabri; Köse, Şükran

doi:10.5222/ankem.2011.173

Ankem

2011

DOI: 10.5222/ankem.2011.173

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Daptomycin Susceptibility in Gram Positive Cocci Isolated from Intensive Care Units

Pınar Şamlıoğlu¹,

Gülfem Ece²,

Sabri Atalay³

et al.

Abstract: ÖZET Anahtar sözcükler: daptomisin, E-test, Gram pozitif koklar, yoğun bakım birimleri SUMMARY Daptomycin Susceptibility in Gram Positive Cocci Isolated from Intensive Care Units The sensitivities of 151 Gram positive cocci isolated from intensive care units of Tepecik Education and Research Hospital between April 16 and December 15, 2010 were determined by E-test for daptomycin and by disk diffusion and Vitek-2 system for other antibiotics. The strains consisted of methicilin sensitive and resistant staphyloc… Show more

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2014

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Daptomycin and tigecycline susceptibility of vancomycin resistant enterococci isolated from rectal swab cultures

Altun¹

2014

Jmid

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The increased incidence of infections by vancomycin-resistant Entero-coccus (VRE) causes an accumulation of patients who are either colonized with VRE or flagged as potentially colonized with VRE. Since such patients require precautionary isolation upon admission to a hospital, rapid methods to establish VRE coloniza-tion status would improve patient care and optimize hospital operation. We evaluated van quantitative PCR (qPCR) on one enrichment broth as a VRE-screening approach. We obtained 255 sets of five rectal specimens from 243 patients. The specimens were cultured using an amoxicillin-containing enrichment broth. Subsequently , a chromogenic agar was incubated and suspect colonies were inoculated on a blood agar plate and characterized by matrix-assisted laser desorption ioniza-tion-time of flight (MALDI-TOF), followed by a vancomycin Etest in cases in which Enterococcus spp. were detected. The culturing results were compared with the outcome of van qPCR on all enrichment broths of the first rectal swab. The van qPCR was positive for 43% of the sample sets (vanA, n 5; vanB, n 101; vanA and vanB, n 3). Based on culture data, 20 (7.8%) of the sets were VRE positive in at least one of five samples. The negative predictive value of van qPCR on the first enrichment broth was 99.3%. With a cutoff quantification cycle (C q) value of 35 to discriminate negative and positive samples, 87% of the negative patients can be identified within a day after obtaining the sample, compared to 7 days in the cultur-ing approach. VRE screening using qPCR on one enrichment broth can quickly identify non-VRE-colonized patients and therefore decrease costs and limit unnecessary isolation restrictions.

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Daptomycin and tigecycline susceptibility of vancomycin resistant enterococci isolated from rectal swab cultures

Altun¹

2014

Jmid

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Daptomycin Susceptibility in Gram Positive Cocci Isolated from Intensive Care Units

Cited by 1 publication

References 14 publications

Daptomycin and tigecycline susceptibility of vancomycin resistant enterococci isolated from rectal swab cultures

Daptomycin and tigecycline susceptibility of vancomycin resistant enterococci isolated from rectal swab cultures

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