Daptomycin and AgNP co-loaded rGO nanocomposites for specific treatment of Gram-positive bacterial infection <i>in vitro</i> and <i>in vivo</i>

Tong, Chunyi; Li, Li; Xiao, Feng; Fan, Jialong; Zhong, Xianghua; Liu, Xuanming; Liu, Bin; Wu, Zhaohui; Zhou, Jian

doi:10.1039/c9bm01229j

Cited by 23 publications

(11 citation statements)

References 49 publications

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“…It has been reported that various materials such as organic compounds (Zhou et al, 2021), metal nanomaterials (Li et al, 2021;Tong et al, 2020) apoptosis (Cui et al, 2012;Tong et al, 2018Tong et al, , 2019Tong et al, , 2020. In this study, we found that ATP level of K. pneumoniae declined about 92.3% after the treatment of OHH NPs + NIR, suggesting the best antibacterial efficacy of this strategy.…”

Section: Antibacterial Mechanism Explorationsupporting

confidence: 55%

Ofloxacin‐loaded HMPB NPs for Klebsiella pneumoniae eradication in the surgical wound with the combination of PTT

Liu

Zhong

Tang

et al. 2022

Biotech & Bioengineering

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Klebsiella pneumoniae (K. pneumoniae) is a common bacterium whose drug-resistant can cause surgical failures and incurable infections in hospital patients. Thus, how to reverse or delay the resistance induction has become a great challenge for development antiresistant drug. Recently, the combination of nanomaterial-loaded antibiotics with photothermal therapy showed the efficient antibacteria ability under a low dosage of antibiotics. In this study, a nanocomposite of HMPB NPs with inherent photothermal therapy capability was used to eradicate K. pneumoniae after loading with Ofloxacin, an antibiotic against K. pneumoniae in vitro and in vivo. The nanocomplexes named as Ofloxacin@HMPB@HA NPs showed a higher effect against K. pneumoniae by destroying cell integrity and inducing ATP leakage with the assistance of laser irradiation, compared with sole Ofloxacin@HMPB@HA NPs or laser irradiation. Surgical wound infection assay further demonstrated the efficient killing K. pneumoniae and promoting the formation of new tissues, as well, which was reflected by the rapid healing of surgical wound. In summary, these results indicate the great potential of this combinational tactic based on Ofloxacin@HMPB@HA NPs for preventing the failure caused by K. pneumoniae infection.

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Section: Antibacterial Mechanism Explorationsupporting

confidence: 55%

Ofloxacin‐loaded HMPB NPs for Klebsiella pneumoniae eradication in the surgical wound with the combination of PTT

Liu

Zhong

Tang

et al. 2022

Biotech & Bioengineering

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“…In general, decreased ATP levels usually indicate that cells undergo apoptosis or necrosis. [ 27 ] As presented in Figure A,B, Ag‐PEG NPs and Ag‐P&C NPs‐treated groups caused 79.82% and 99.89% decrease in ATP levels in MRSA , and ≈95.33% and 98.99% in E. coli , respectively. These results suggest the stronger inhibitory effect of Ag NPs on the ATP levels in E. coli cells.…”

Section: Resultsmentioning

confidence: 95%

pH‐Triggered Size‐Tunable Silver Nanoparticles: Targeted Aggregation for Effective Bacterial Infection Therapy

Cheng

Pei

Xie

et al. 2022

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The rapid spread of drug‐resistant pathogens threatens human health. To address the current antibacterial dilemma, the development of antibiotic‐free strategies using nanotechnology is imperative. In this study, silver nanoparticles (Ag‐P&C NPs) with pH‐sensitive charge reversal and self‐aggregation capacities are successfully synthesized. In the acidic microenvironment of bacterial biofilms, protonation of the surface peptide enhances the affinity of Ag‐P&C NPs for bacteria, which can make Ag‐P&C NPs prone to target and penetrate into biofilms, and the self‐aggregated capacity helps Ag‐P&C NPs remain in biofilms for a long time to disrupt bacterial biofilm formation. In addition, biocompatible Ag‐P&C NPs are utilized in three types of bacteria‐infected animal models. They exhibit an excellent performance in killing bacteria, inhibiting plaque biofilms, and ameliorating inflammatory responses. In conclusion, this study offers new insights into antibiotic‐free antibacterial strategies, and exhibits promising application prospects.

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“…Prior to investigate the capability of GFV probe to monitor the efficacy of antibiotic treatment in vivo, the curative effect of Dap to S. aureus in vivo was studied. [ 39 ] Dap treated S. aureus infection for different days (1, 2, 3, 4, 5, 6, and 7 d) were determined by counting bacterial colonies formed on the LB‐agar plates derived from the homogenized tissue from the infected sites of mice. Figure S20 (Supporting Information) showed that the number of S. aureus at the infected site decreased with increasing in days of administration of Dap , which indicated that continuous administration of Dap could effectively treat S. aureus infectious disease, and after 7 d of Dap treatment, the number of bacteria at the infected site was about 2 × 10 5 cfu, which was within the detection range of the GFV probe.…”

Section: Resultsmentioning

confidence: 99%

Bacteria‐Targeted MRI Probe‐Based Imaging Bacterial Infection and Monitoring Antimicrobial Therapy In Vivo

Hao

et al. 2021

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Despite the significant advances of imaging techniques nowadays, accurate diagnosis of bacterial infections and real‐time monitoring the efficacy of antibiotic therapy in vivo still remain huge challenges. Herein, a self‐assembling peptide (FFYEGK) and vancomycin (Van) antibiotic molecule co‐modified gadolinium (Gd) MRI nanoaggregate probe (GFV) for detecting Staphylococcus aureus (S. aureus) infection in vivo and monitoring the treatment of S. aureus‐infected myositis by using daptomycin (Dap) antibiotic as model are designed and fabricated. The as‐prepared GFV probe bears Van molecules, making itself good bacteria‐specific targeting, and the peptide in the probe can enhance the longitudinal relaxivity rate (r1) after self‐assembly due to the π–π stacking. The study showed that, based on the GFV probe, bacterial infections and sterile inflammation can be discriminated, and as few as 105 cfu S. aureus can be detected in vivo with high specificity and accurately. Moreover, the T1 signal of GFV probe at the S. aureus‐infected site in mice correlates with the increasing time of Dap treating, indicating the possibility of monitoring the efficacy of antibacterial agents for infected mice based on the as proposed GFV probe. This study shows the potential of GFV probe for diagnosis, evaluation, and prognosis of infectious diseases in clinics.

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Daptomycin and AgNP co-loaded rGO nanocomposites for specific treatment of Gram-positive bacterial infection in vitro and in vivo

Abstract: rGO was used for simultaneously anchoring AgNPs and Daptomycin to prepare rGO@Ag@Dap for anti-bacterium. The new nanomaterial showed strong Gram-positive bacteria killing ability in vitro and enhanced wound healing infected with S. aureus in vivo.

Cited by 23 publications

References 49 publications

Ofloxacin‐loaded HMPB NPs for Klebsiella pneumoniae eradication in the surgical wound with the combination of PTT

Ofloxacin‐loaded HMPB NPs for Klebsiella pneumoniae eradication in the surgical wound with the combination of PTT

pH‐Triggered Size‐Tunable Silver Nanoparticles: Targeted Aggregation for Effective Bacterial Infection Therapy

Bacteria‐Targeted MRI Probe‐Based Imaging Bacterial Infection and Monitoring Antimicrobial Therapy In Vivo

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