Dapsone Attenuates the Severity of Gastric Erosion Induced by Ethanol; Stress; and Indomethacin in Rats: A Pharmacological Approach

Abstract

Background: Dapsone, as an antimicrobial compound, has shown strong anti-inflammatory properties in clinical and experimental studies. Inflammation in stomach mocusa produce gastric erosion. Several factors contribute to the development of gastric erosions, including corticosteroids and nonsteroidal anti-inflammatory drugs (NSAIDs), alcohol, and stress. The aim of this study, therefore, was to evaluate the effects of dapsone against gastric erosions-induced by alcohol, stress, or indomethacin in rat.Methods: Gastric damage was induced in male rats in different three experimental models including ethanol (5 ml/kg, p.o.)-, water-immersion stress-, and indomethacin (30 mg/kg, p.o.)- induced gastric injury. Macroscopic lesion scores (the J- score), inflammatory cytokines levels, NF-κB protein expression, MPO activity, and histopathological evaluation were assessed. Results: Induction of erosion in gastric mocusal led to a significant increase in J-score as an index of macroscopic parameter, tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) as biochemical markers, NF-κB and myeloperoxidase (a marker of neutrophil activation, as well microscopic deterioration. Dapsone reduced the tissue injuries and erosion area, serum levels of inflammatory cytokines, the expression of NF-κB and tissue concentration of myeloperoxidase (a marker of neutrophil activation) in all three experimental groups. Furthermore, the histopathological studies revealed the reduction of damages in dapsone treated animals.Conclusions: To conclude, dapsone exhibits significant gastro-protective effects against the development of experimental gastric erosions in rats which seems to be related to its anti-inflammatory properties.

WITHDRAWN: Repurposing of Dapsone as Neuroprotective Agent on Cuprizone-induced Demyelination via Targeting Nrf2 and NF-kB in C57BL/6 Mice

“…DAP treatment during the last 2 weeks of the experimental procedure signi cantly reduced p-IKB levels suggesting a reduced activation of NF-kB and in ammation. Previous studies have been reported the inhibitory effects of DAP on NF-kB function and the reduction of pro-in ammatory cytokines [19,23].…”

“…Thus, the upregulation of Nrf 2 may be useful in MS therapy due to the induction of protective enzymes and the reduction of oxidative stress. Dapsone (DAP), a diaminodiphenyl sulfone, exerts antimicrobial and anti-protozoa effects [19]. This sulfonamide related drug is one of the important medication for the treatment of leprosy in [20].…”