Daphnia magna metabolic profiling as a promising water quality parameter for the biological early warning system

Jeong, Tae-Yong; Simpson, Myrna J.

doi:10.1016/j.watres.2019.115033

Cited by 26 publications

(14 citation statements)

References 25 publications

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“…Propranolol has been reported to affect lipid metabolism in the freshwater crustacean Daphnia magna (Taylor et al, 2010;Fuertes et al, 2020), which is in line with the observed effects on glycerophospholipid pathways. There is also reported information that propranolol affected branched amino acids such as valine, leucine and isoleucine (Jeong and Simpson, 2019), which is in line with the Valine, leucine and isoleucine biosynthesis pathways reported in Table S1 The metabolites showing the highest degrees of up-regulation upon propranolol treatments were phenylalanine, inosine and thymidine. There is reported information that propranolol enhances adenine nucleotide degradation in human muscle during exercise resulting in increasing levels of inosine (Broberg et al, 1988), and of b-Adrenergic regulation of certain amino acid transport (i.e Phe) across the blood-brain barrier (Takao et al, 1992).…”

Section: Biochemical Interpretation Of the Observed Metabolite Changessupporting

confidence: 78%

Untargeted metabolomics changes on Gammarus pulex induced by propranolol, triclosan, and nimesulide pharmaceutical drugs

et al. 2020

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Section: Biochemical Interpretation Of the Observed Metabolite Changessupporting

confidence: 78%

Untargeted metabolomics changes on Gammarus pulex induced by propranolol, triclosan, and nimesulide pharmaceutical drugs

et al. 2020

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“…Metabolomic analysis in daphnids has been highlighted as a key mechanistic tool for environmental monitoring [ 57 ] and can provide valuable fitness information at the molecular level [ 58 ]. Our study verified that simple biochemical markers of enzyme activities show similar patterns in changes with sensitive holistic metabolomic analysis.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Michalaki

McGivern²,

Poschet³

et al. 2022

Toxics

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The continuous global increase in population and consumption of resources due to human activities has had a significant impact on the environment. Therefore, assessment of environmental exposure to toxic chemicals as well as their impact on biological systems is of significant importance. Freshwater systems are currently under threat and monitored; however, current methods for pollution assessment can neither provide mechanistic insight nor predict adverse effects from complex pollution. Using daphnids as a bioindicator, we assessed the impact in acute exposures of eight individual chemicals and specifically two metals, four pharmaceuticals, a pesticide and a stimulant, and their composite mixture combining phenotypic, biochemical and metabolic markers of physiology. Toxicity levels were in the same order of magnitude and significantly enhanced in the composite mixture. Results from individual chemicals showed distinct biochemical responses for key enzyme activities such as phosphatases, lipase, peptidase, β-galactosidase and glutathione-S-transferase. Following this, a more realistic mixture scenario was assessed with the aforementioned enzyme markers and a metabolomic approach. A clear dose-dependent effect for the composite mixture was validated with enzyme markers of physiology, and the metabolomic analysis verified the effects observed, thus providing a sensitive metrics in metabolite perturbations. Our study highlights that sensitive enzyme markers can be used in advance on the design of metabolic and holistic assays to guide the selection of chemicals and the trajectory of the study, while providing mechanistic insight. In the future this could prove to become a useful tool for understanding and predicting freshwater pollution.

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“…The normalized concentration data points were then scaled using the autoscaling function included in the MetaboAnalystR package [89,93]. The methods previously described have been used across metabolomics experiments with varying contaminants and target organisms [51,53,57,94].…”

Section: Data Processing and Pathway Analysismentioning

confidence: 99%

“…Here liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to decode the metabolic responses of D. magna to three commonly observed HAAs (DCAA, TCAA and DBAA) in acute sub-lethal exposure studies. This method targets amino acids and derivatives, neurotransmitters, nucleosides/nucleotides, saccharide derivatives, vitamins, polyamines and carboxylic acids and has been used to assess sub-lethal toxicity in previous studies [51][52][53][54]. Previous studies reported that exposure to HAAs leads to disruptions in energy metabolism [23,24,39,41], and, as such, it is hypothesized that D. magna will also exhibit energy disruption with contaminant exposure.…”

Section: Introductionmentioning

confidence: 99%

Targeted Metabolomic Assessment of the Sub-Lethal Toxicity of Halogenated Acetic Acids (HAAs) to Daphnia magna

Labine

Simpson

2021

Metabolites

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Halogenated acetic acids (HAAs) are amongst the most frequently detected disinfection by-products in aquatic environments. Despite this, little is known about their toxicity, especially at the molecular level. The model organism Daphnia magna, which is an indicator species for freshwater ecosystems, was exposed to sub-lethal concentrations of dichloroacetic acid (DCAA), trichloroacetic acid (TCAA) and dibromoacetic acid (DBAA) for 48 h. Polar metabolites extracted from Daphnia were analyzed using liquid chromatography hyphened to a triple quadrupole mass spectrometer (LC-MS/MS). Multivariate analyses identified shifts in the metabolic profile with exposure and pathway analysis was used to identify which metabolites and associated pathways were disrupted. Exposure to all three HAAs led to significant downregulation in the nucleosides: adenosine, guanosine and inosine. Pathway analyses identified perturbations in the citric acid cycle and the purine metabolism pathways. Interestingly, chlorinated and brominated acetic acids demonstrated similar modes of action after sub-lethal acute exposure, suggesting that HAAs cause a contaminant class-based response which is independent of the type or number of halogens. As such, the identified metabolites that responded to acute HAA exposure may serve as suitable bioindicators for freshwater monitoring programs.

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Daphnia magna metabolic profiling as a promising water quality parameter for the biological early warning system

Cited by 26 publications

References 25 publications

Untargeted metabolomics changes on Gammarus pulex induced by propranolol, triclosan, and nimesulide pharmaceutical drugs

Untargeted metabolomics changes on Gammarus pulex induced by propranolol, triclosan, and nimesulide pharmaceutical drugs

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Targeted Metabolomic Assessment of the Sub-Lethal Toxicity of Halogenated Acetic Acids (HAAs) to Daphnia magna

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