Daphnetin ameliorates 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis through Nrf-2-Keap1 and NF-κB pathways

Daphnetin-mediated Nrf2 antioxidant signaling pathways ameliorate tert-butyl hydroperoxide (t-BHP)-induced mitochondrial dysfunction and cell death

“…Increasing evidence shows that Nrf2-mediated antioxidant signaling pathways are crucial for the prevention of cell injury resulting from oxidative stress [27]. Daph, a phytochemical that belongs to the coumarin compound, exists ubiquitously in the human diet and could upregulate Nrf2 and HO-1 protein expression to enhance antioxidant potentiation during carcinogenesis in rats [24]. In the present study, we focused on investigating the potential cytoprotective effects of Daph against t-BHP-induced oxidative damage, mitochondrial dysfunction, cell apoptosis and its underlying molecular .…”

“…In fact, recent studies have shown that numerous bioactive molecules, including natural compounds, were dependent on Nrf2 activation to induce various antioxidant enzymes expression, such as HO-1, GCLM, GCLC and NQO1, which are essential for inhibiting redox impairment and mitochondrial dysfunction [43][44][45]. It has been reported that Daph could protect from carcinogenesis via upregulation of Nrf2 and HO-1 protein expression and enhance the ability of potential antioxidants in rats [24]. To further investigate the protective effect of Daph against oxidative damage and mitochondrial dysfunction associated with Nrf2 activation, we observed the effect of Daph on the Keap1/Nrf2/ARE antioxidant pathway and the regulation of antioxidant enzymes.…”

“…1A), a natural coumarin derivative, is isolated from plants of the genus Daphne and has been used clinically in the treatment of various diseases, such as rheumatoid arthritis and coagulation disorders [22]. Furthermore, recent reports have suggested that Daph effectively attenuates oxidative stress-related hepatotoxicity and inhibits DMBA-induced mammary carcinogenesis by activating the Nrf2 signaling pathway [23,24]. However, the cytoprotective effect of Daph against oxidative damage and mitochondrial dysfunction and the underlying molecular mechanisms have yet to be elucidated.…”

Daphnetin inhibits RANKL‐induced osteoclastogenesis in vitro

“…7,8 Daphnetin has been shown to possess broad bioactivities such as antioxidative, anti-inflammatory, antitumor, and antiarthritis effects. [9][10][11][12][13] However, the role of daphnetin in osteoclast formation remains unclear. In this study, we investigated the effects of daphnetin on RANKL-induced osteoclastogenesis using bone marrow-derived macrophages (BMMs).…”

Quercetin loaded folate targeted plasmonic silver nanoparticles for light activated chemo-photothermal therapy of DMBA induced breast cancer in Sprague Dawley rats

“…† The method for breast cancer induction in the SD rats, as well as the duration of the treatment with various drug formulations has been selected and performed following previously published studies. [41][42][43][44][45][46] Control rats in group I were administered with only the vehicle (olive oil (0.5 ml) on the day of induction and the treatment involved 1% sodium carboxymethyl cellulose (CDH, New Delhi, India)). Cancer was induced in SD rats belonging to group II (induced control) through the slightly modied air pouch technique 40 and they received 20 mg DMBA (suspended in 0.5 ml olive oil) on day 0.…”