Danielozyma pruni sp. nov., an asexual yeast species isolated from insect frass

Abstract

Two strains (NYNU 218101 and NYNU 218104) of an asexual yeast species were isolated from insect frass collected in insect tunnels of red leaf plum trees in the Henan Province, central China. Molecular phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region showed that these two strains belonged to the genus Danielozyma, with Danielozyma litseae as the closest known species. They differed from the type strain of D. litseae by 0.6 % substitutions (three substitutions and one gap) in the D1/D2 domain and by 5.1 % substitutions (19 substitutions and six gaps) in the ITS region, respectively. When compared with the partial ACT1, TEF1 and RPB1 gene sequences, they differed by 3 % (26 substitutions), 2.7 % (25 substitutions) and 9 %(54 substitutions) from D. litseae NRRL YB-3246T in these regions. Physiologically, they also differed from its closest known species D. litseae based on the ability to assimilate inulin and galactitol, as well as to grow in 0.1 % cycloheximide and its inability to ferment maltose and raffinose. In order to classify the two new isolates based on morphological and molecular evidence, we proposed the description of a novel species Danielozyma pruni sp. nov. with strain JCM 35735T as holotype (Mycobank MB 849101).

Diversity and distribution of yeasts in intertidal zones of China

“…In this study, we obtained 286 yeast species, including 140 ascomycetous and 146 basidiomycetous species associated with intertidal zones of China (Table S2). Boekhout et al (2022) summarized that at least 782 yeast species have been recorded from China recorded until 2020 and 49 additional yeast species were reported from China in the past three years (Gao et al, 2021;Shi et al, 2021;Chai et al, 2022a;Chai et al, 2022b;Chai et al, 2022c;Chu et al, 2022;Li et al, 2022;Wei et al, 2022;Chai et al, 2023;Liu et al, 2023;Qiao et al, 2023;Yu et al, 2023;Zhu et al, 2023a;Zhu et al, 2023b). The number of yeast species discovered in this study accounts for 34.7% in the total yeast species that have been found from China, indicating that there are abundant resources of culturable yeasts in the intertidal zones of China.…”