Danggui Buxue Tang attenuates eosinophil infiltration and airway hyperresponsiveness in asthmatic mice

Lin, Ching-Che; Liou, Chian-Jiun; Chiang, Chien-Ying; Wen-ying, Huang; Huang, Wen‐Chung

doi:10.1016/j.anai.2011.08.006

Cited by 10 publications

(11 citation statements)

References 27 publications

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“…Danggui Buxue Tang (DBT), which contains Astragalus membranaceus (AM) and Angelica sinensis (AS) (AM : AS = 5 : 1), was prepared as previously described [ 11 ]. In brief, AS and AM were planted and collected from Gansu and Shanxi province, respectively, China.…”

Section: Methodsmentioning

confidence: 99%

“…Blood was collected and centrifuged to harvest serum. Serum and spleen cells were isolated as previously described [ 11 ], and cells (5 × 10 6 cells/mL) were then stimulated with 2 μ g/mL concanavalin A (Con A) for 2 days in RPMI 1640 medium. The supernatants were collected and cytokine levels measured by ELISA.…”

Section: Methodsmentioning

confidence: 99%

“…Danggui Buxue Tang (DBT) is often used in Chinese medicine in China and Taiwan [ 11 ]. It consists of Angelica sinensis and Astragalus membranaceus (1 : 5) and is predominately used to increase blood circulation and qi [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Our previous study found that DBT significantly suppresses airway hyperresponsiveness and eosinophil infiltration in mice by blocking Th2 cytokine production. Because AD is a disease of excessive Th2 cytokine production [ 11 ], we evaluated whether DBT has a therapeutic effect by suppressing this response in AD-like skin lesions in mice.…”

Section: Introductionmentioning

confidence: 99%

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Danggui Buxue Tang Inhibits 2,4-Dinitrochlorobenzene: Induced Atopic Dermatitis in Mice

Fang

Cheng

Hwang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Danggui Buxue Tang (DBT) is a herbal decoction that has been used in Chinese medicine to enhance qi and blood circulation. Previously, we found that DBT can suppress allergy-related asthma in mice, leading us to hypothesize that DBT might ameliorate allergy disease. In this study, we evaluated whether DBT can attenuate atopic dermatitis (AD) symptoms and have an anti-inflammatory effect on AD-like mice. The dorsal skin of female mice was shaved and sensitized cutaneously (skin smear) with 1-chloro-2,4-dinitrobenzene. Mice were then given various doses of DBT from days 14 to 29 cutaneously. DBT treatment suppressed ear swelling and skin inflammation and decreased mast cell and eosinophil infiltration into skin and ear tissue. DBT also inhibited levels of IgE and Th2-associated cytokine levels in serum. These results demonstrate that cutaneous administration of DBT reduced the development of AD-like skin lesions in mice.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Danggui Buxue Tang Inhibits 2,4-Dinitrochlorobenzene: Induced Atopic Dermatitis in Mice

Fang

Cheng

Hwang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…Cell culture supernatants were assayed using enzyme-linked immunosorbent assay (ELISA) kits for IL-4, IL-5, IL-6, IL-13, CCL11, CCL24, and ICAM-1 (R&D Systems, Minneapolis, MN, USA), as described previously 19,21. Serum OVA-specific antibodies, IgG1, IgG2a, and IgE, were measured using a specific ELISA kit (BD Biosciences).…”

Section: Methodsmentioning

confidence: 99%

Oral Lovastatin Attenuates Airway Inflammation and Mucus Secretion in Ovalbumin-Induced Murine Model of Asthma

Liou

Cheng

Huang

et al. 2014

Allergy Asthma Immunol Res

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PurposeLovastatin is an effective inhibitor of cholesterol synthesis. A previous study demonstrated that lovastatin can also suppress airway hyperresponsiveness (AHR) in murine model of asthma. We aimed to investigate the effect of lovastatin on mucus secretion and inflammation-associated gene expression in the lungs of murine model of asthma.MethodsFemale BALB/c mice were sensitized and challenged with ovalbumin (OVA) by intraperitoneal injection, and orally administered lovastatin from days 14 to 27 post-injection. Gene expression in lung tissues was analyzed using real-time polymerase chain reaction. AHR and goblet cell hyperplasia were also examined. BEAS-2B human bronchial epithelial cells were used to evaluate the effect of lovastatin on the expression of cell adhesion molecules, chemokines, and proinflammatory cytokines in vitro.ResultsWe showed that lovastatin inhibits the expression of Th2-associated genes, including eotaxins and adhesion molecules, in the lungs of murine model of asthma. Mucin 5AC expression, eosinophil infiltration and goblet cell hyperplasia were significantly decreased in the lung tissue of murine model of asthma treated with lovastatin. Furthermore, lovastatin inhibited AHR and expression of Th2-associated cytokines in bronchoalveolar lavage fluid. However, a high dose (40 mg/kg) of lovastatin was required to decrease specific IgE to OVA levels in serum, and suppress the expression of Th2-associated cytokines in splenocytes. Activated BEAS-2B cells treated with lovastatin exhibited reduced IL-6, eotaxins (CCL11 and CCL24), and intercellular adhesion molecule-1 protein expression. Consistent with this, lovastatin also suppressed the ability of HL-60 cells to adhere to inflammatory BEAS-2B cells.ConclusionsThese data suggest that lovastatin suppresses mucus secretion and airway inflammation by inhibiting the production of eotaxins and Th2 cytokines in murine model of asthma.

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Immune Homeostasis: Effects of Chinese Herbal Formulae and Herb-Derived Compounds on Allergic Asthma in Different Experimental Models

Liu

Wang

et al. 2018

Chin. J. Integr. Med.

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Allergic asthma is thought to arise from an imbalance of immune regulation, which is characterized by the production of large quantities of IgE antibodies by B cells and a decrease of the interferon-γ/interleukin-4 (Th1/Th2) ratio. Certain immunomodulatory components and Chinese herbal formulae have been used in traditional herbal medicine for thousands of years. However, there are few studies performing evidence-based Chinese medicine (CM) research on the mechanisms and effificacy of these drugs in allergic asthma. This review aims to explore the roles of Chinese herbal formulae and herb-derived compounds in experimental research models of allergic asthma. We screened published modern CM research results on the experimental effects of Chinese herbal formulae and herb-derived bioactive compounds for allergic asthma and their possible underlying mechanisms in English language articles from the PubMed and the Google Scholar databases with the keywords allergic asthma, experimental model and Chinese herbal medicine. We found 22 Chinese herb species and 31 herb-derived anti-asthmatic compounds as well as 12 Chinese herbal formulae which showed a reduction of airway hyperresponsiveness, allergen-specifific immunoglobulin E, inflflammatory cell infifiltration and a regulation of Th1 and Th2 cytokines in vivo, in vitro and ex vivo, respectively. Chinese herbal formulae and herbderived bioactive compounds exhibit immunomodulatory, anti-inflflammatory and anti-asthma activities in different experimental models and their various mechanisms of action are being investigated in modern CM research with genomics, proteomics and metabolomics technologies, which will lead to a new era in the development of new drug discovery for allergic asthma in CM.

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Danggui Buxue Tang attenuates eosinophil infiltration and airway hyperresponsiveness in asthmatic mice

Cited by 10 publications

References 27 publications

Danggui Buxue Tang Inhibits 2,4-Dinitrochlorobenzene: Induced Atopic Dermatitis in Mice

Danggui Buxue Tang Inhibits 2,4-Dinitrochlorobenzene: Induced Atopic Dermatitis in Mice

Oral Lovastatin Attenuates Airway Inflammation and Mucus Secretion in Ovalbumin-Induced Murine Model of Asthma

Immune Homeostasis: Effects of Chinese Herbal Formulae and Herb-Derived Compounds on Allergic Asthma in Different Experimental Models

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