Dandelion sterol improves diabetes mellitus–induced renal injury in in vitro and in vivo study

Tian, Lin; Fu, Peng; Zhou, Min; Qi, Jiping

doi:10.1002/fsn3.2491

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…43 Taraxacum officinale Weber and its sterols have therapeutic effects on diabetes induced renal injury through in vitro and in vivo studies. 44 CILF is a representative prescription consisting of seven herbs and it is clinically used to treat patients with gout and hyperuricemia in TCM. Although the individual drugs in CILF have various effects, such as anti-inflammatory, antioxidant 45 and hyperuricemia effects, 41 as demonstrated by in experiments, we revealed that CILF significantly decreased UA and damage in kidney tissues.…”

Section: Discussionmentioning

confidence: 99%

A Novel Approach Based on Gut Microbiota Analysis and Network Pharmacology to Explain the Mechanisms of Action of Cichorium intybus L. Formula in the Improvement of Hyperuricemic Nephropathy in Rats

Amatjan¹,

Li²,

He³

et al. 2023

DDDT

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Background: Cichorium intybus L. formula (CILF) is a traditional Chinese medicine (TCM) widely used in the treatment of gout and hyperuricemic nephropathy (HN). The aim of this research was to investigate the potential protective effect of CILF against HN and elucidated the underlying mechanism. Methods: CILF water extract was administered to an HN rat model established by adenine combined with ethambutol. The levels of uric acid (UA), serum urea nitrogen (UREA), and creatinine (CREA) were detected. Changes in the pathology and histology of the kidney were observed by hematoxylin-eosin staining. The 16S rRNA of the gut microbiota was sequenced. The binding ability of the main ingredients of CILF to key targets was analyzed by network pharmacology and molecular docking. The expression levels of the related mRNAs and proteins in the kidney were evaluated by RT-qPCR and immunohistochemistry analysis. Results: CILF administration significantly alleviated increases in UA, UREA, and CREA, structural damage, and kidney dysfunction. Gut microbiota analysis was applied to explore the pharmacological mechanism of the effects of CILF on bacterial diversity and microbiota structure in HN. CILF decreased the abundance of Bacteroides. In addition, it increased the abundance of Lactobacillaceae, Erysipelotrichaceae, Lachnospiraceae, Ruminococcaceae, and Bifidobacterium. Based on network pharmacology and molecular docking analysis, CILF profoundly influenced the IL17, TNF and AGE-RAGE signaling pathway. Additionally, CILF inhibited the expression of STAT3, VEGFA and SIRT1 to improve the symptoms of nephropathy. Our research suggested that CILF protects against kidney dysfunction in rats with HN induced by adenine combined with ethambutol. Conclusion: Our findings on the anti-HN effects of CILF and its mechanism of action, from the viewpoint of systems biology, and elaborated that CILF can alter the diversity and community structure of the gut microbiota in HN, providing new approaches for the prevention and treatment of HN.

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Section: Discussionmentioning

confidence: 99%

A Novel Approach Based on Gut Microbiota Analysis and Network Pharmacology to Explain the Mechanisms of Action of Cichorium intybus L. Formula in the Improvement of Hyperuricemic Nephropathy in Rats

Amatjan¹,

Li²,

He³

et al. 2023

DDDT

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“…In addition, it has been observed in vitro and in vivo that dandelion can suppress the production of tumor necrosis factor (TNFα) and interleukin-6 (IL-6). The animal model of diabetes mellitus-induced renal injury can be successfully treated with dandelion by reducing the production of interleukin-6 and TNF-α [5].…”

Section: Introductionmentioning

confidence: 99%

Effects of dandelion root on rat heart function and oxidative status

Radoman

Živković

Zdravković

et al. 2023

BMC Complement Med Ther

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This study aimed to examine the effects of dandelion root on rat heart function and oxidative status. At the beginning of the experimental protocol, Wistar albino rats were randomly classified into two groups (10 rats per group): 1. control group – animals that drank tap water; 2. experimental group – animals that drank dandelion root for four weeks. Every morning for four weeks, the animals received freshly boiled dandelion root in a volume of 250 ml. At the end of the dandelion administration, animals were sacrificed, and their hearts were isolated and retrogradely perfused according to the Langendorff technique at a gradually increasing perfusion pressure between 40 – 120 cm H2O. The following myocardial function parameters were measured: maximum rate of left ventricular pressure development (dp/dt max), minimum rate of left ventricular pressure development (dp/dt min), systolic left ventricular pressure (SLVP), diastolic left ventricular pressure (DLVP), heart rate (HR). In addition, the coronary flow (CF) was measured flowmetrically. Finally, blood samples were collected after sacrificing to determine oxidative stress biomarkers: nitrite (NO2−), superoxide anion radical (O2−), hydrogen peroxide (H2O2), the index of lipid peroxidation (TBARS), reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD). The present pioneer results indicated that dandelion root did not manifest a negative impact on functional aspects of isolated rat heart. In addition, dandelion consumption was not associated with promising results in terms of maintaining systemic redox balance.

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CircACTR2 attenuated the effects of tetramethylpyrazine on human kidney cell injury

Chen,

Zou,

Yang

2024

J Bioenerg Biomembr

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Dandelion sterol improves diabetes mellitus–induced renal injury in in vitro and in vivo study

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 6 publications

References 32 publications

A Novel Approach Based on Gut Microbiota Analysis and Network Pharmacology to Explain the Mechanisms of Action of Cichorium intybus L. Formula in the Improvement of Hyperuricemic Nephropathy in Rats

A Novel Approach Based on Gut Microbiota Analysis and Network Pharmacology to Explain the Mechanisms of Action of Cichorium intybus L. Formula in the Improvement of Hyperuricemic Nephropathy in Rats

Effects of dandelion root on rat heart function and oxidative status

CircACTR2 attenuated the effects of tetramethylpyrazine on human kidney cell injury

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