Dammaradiene synthase, a squalene cyclase, from Dryopteris crassirhizoma Nakai

Photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity

“…Powdered and dried rhizomes of various Dryopteris ferns were formerly used as remedies for helminthiasis caused by Diphyllobothrium latum [16,17]. Helmintic activity was attributed to acylphloroglucinol derivatives, such as albaspidins [16,17].…”

“…Powdered and dried rhizomes of various Dryopteris ferns were formerly used as remedies for helminthiasis caused by Diphyllobothrium latum [16,17]. Helmintic activity was attributed to acylphloroglucinol derivatives, such as albaspidins [16,17]. Earlier studies on chemical constituents of D. crassirhizoma collected in Japan resulted in the isolation of 12 triterpenes and several acylphloroglucinols from dried and fresh leaflets [17,18].…”

“…Helmintic activity was attributed to acylphloroglucinol derivatives, such as albaspidins [16,17]. Earlier studies on chemical constituents of D. crassirhizoma collected in Japan resulted in the isolation of 12 triterpenes and several acylphloroglucinols from dried and fresh leaflets [17,18].…”

Migrated Hopene Synthases from Colysis pothifolia and Identification of a Migration Switch Controlling the Number of 1,2‐Hydride and Methyl Shifts

“…Cloning and sequenceanalysis of SCsf rom two Colysis ferns In order to obtain ap artial sequence, nested PCR was performed with as et of degenerate primers designed from conserved SC amino acid sequences [9,10] and cDNAs isolated from the above-ground part of C. elliptica and C. pothifolia.P CR products of the expected size (ca. 450 bp) were isolated, cloned, and propagated in Escherichia coli.P ositive transformants were selected,a nd the inserted fragments were sequenced.…”

“…The SCs from ferns characterizedt hus far produce 22hydroxyhopane, [9] dammara-18(28),21-diene, [10] germanicene, [11] and tirucalla-7,21-diene, [11] whereas all the functionally characterized SCs from bacteria are SHCs, the product of whichi s hop-22(29)-ene (7). Ferns have also been shown to be as ource of SCs with more variablec atalytic properties than those derived from bacteria.…”