Daming capsule restores endothelial dysfunction induced by high-fat diet

Zhang, Rong; Niu, Huifang; Wang, Ning; Sun, Lihua; Xu, Yi; Zhao, Rong; Ban, Xiang; Yu, Yao; Yang, Baofeng; Ai, Jing

doi:10.1186/1472-6882-12-21

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…Additionally, two proteins, NOS3 and IGF1, were present in the network. This is in-line with the protective effects of DMC against endothelial dysfunction of the aorta in high fat diet rats via upregulation of NOS3 expression [ 19 ] and the beneficial effects on diabetic symptoms [ 17 ]. Taken together, we hypothesized that DMC is a protective agent against CCI.…”

Section: Discussionsupporting

confidence: 70%

“…Four proteins, p53, MMP1, MMP3, and MMP9, are involved in the networks. Moreover, DMC could abate myocardial cell inflammation [ 18 ] and reduce vascular endothelial injury [ 19 ]. Consistently, the related proteins, such as IL6, IL1B, TNF, ACE, EDN1, VEGFA, and ICAM1, could be seen in our networks.…”

Section: Discussionmentioning

confidence: 99%

“…Daming capsule (DMC), a multi-herbal formula, has been used clinically to treat hyperlipidemia for years in China; it is composed of four herbs, including Rheum palmatum L. , Cassia obtusifolia L. , Salvia miltiorrhiza , and Panax ginseng C.A . DMC was reported to have multiple pharmacological activities, such as preventing cardiac dysfunction [ 12 ], resisting free radicals [ 13 , 14 ], improving lipidemia conditions [ 15 , 16 ], decreasing blood glucose levels [ 17 ], protecting against myocardial ischemia reperfusion injury, reducing myocardial infarctions [ 18 , 14 ], and restoring aortic endothelial dysfunction [ 17 , 19 ]. Rheum palmatum L. , the monarch component of DMC, was validated to promote blood circulation, dissipate blood stasis, and protect rats with cerebral ischemia [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of Daming capsule against chronic cerebral ischemia

Song

Zhu

et al. 2015

BMC Complement Altern Med

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BackgroundAccumulating evidence has shown that chronic cerebral ischemia (CCI) is one of the major causes of vascular dementia (VD) characterized by dysregulated cholesterol homeostasis and lipoprotein disturbances. Positive value of lipid-lowering agents has been widely evaluated for the treatment of VD. In the present study, we investigated whether Daming capsule (DMC) protected against CCI-induced VD and its possible mechanisms of action. DMC is a multi-herbal formula composed of Rheum palmatum L., Cassia obtusifolia L., Salvia miltiorrhiza, and Panax ginseng C.A., which has been used to treat hyperlipidemia for years in China.MethodsA network pharmacology method was established to reveal whether DMC contained any chemical constituent targeting CCI-related proteins. Furthermore, the potential anti-CCI effects of DMC (100 mg/kg or 200 mg/kg) administered for 30 days were investigated in vivo on rats that were subjected to permanent bilateral occlusion of the carotid arteries (2-VO). Spatial learning and memory abilities were evaluated using a Morris water maze (MWM) and morphological changes of cerebral cortex and hippocampus were assessed using hematoxylin and eosin staining. Moreover, the lipid peroxidation levels and antioxidative capabilities were measured using biochemical analysis.ResultsOur network pharmacology analysis revealed the existence of multiple CCI-related chemical-target interactions in DMC, suggesting a potential protective effect. An in vivo experiment verified that 200 mg/kg DMC improved cognitive deficits of 2-VO rats in the MWM test and attenuated pathological alterations in both the cerebral cortex and the hippocampus. Biochemical assays indicated that DMC decreased malondialdehyde levels and CCI-elevated superoxide dismutase activities, but increased the activities of glutathione peroxidase and catalase.ConclusionsOur findings suggested that DMC protected against cognitive dysfunction and nerve injuries caused by CCI, which is most likely related to its antioxidant actions.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective effect of Daming capsule against chronic cerebral ischemia

Song

Zhu

et al. 2015

BMC Complement Altern Med

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“…Meanwhile, histological examination of H&E‐stained aortic sections from groups II and III showed that HFD resulted in extensive severe injury in the endothelial tissue through detachment of the endothelial layer and infiltration of the inflammatory cells. HFDs affect vascular function by causing impaired vascular responsiveness and elasticity (Zhang et al, ). Rats receiving the anti‐inflammatory diet (group IV) showed a nearly normal structure and the HFD‐fed rats that received celecoxib + ω‐3 PUFA (group VΙΙ) and ω‐3 PUFA and safranal (group VΙΙΙ) demonstrated an improvement in the endothelial layer compared with groups II and III.…”

Section: Discussionmentioning

confidence: 99%

Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity

Ghazala

Medney

Meleis

et al. 2020

Biomedical Chromatography

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Lipotoxicity is defined as deposition of excess fat associated with an inflammatory response. Metabolomic analysis of fatty acids (FAs) can be a marker of silent inflammation. ω3‐Enriched diet, celecoxib, and safranal may have a protective anti‐inflammatory role. In this work, total FAs extracted from red blood cells and arachidonic acid‐to‐eicosapentaenoic acid (AA‐to‐EPA) ratios were assessed using GC–MS assay in single‐ion monitoring mode. The study was conducted on 64 male rats divided into eight groups: I, controls; II, rats received high‐fat diet (HFD), III, rats received ω‐6‐enriched HFD; IV, rats received ω‐3‐enriched HFD; V, rats received celecoxib with HFD; VI, rats received safranal with HFD; VII and VIII, rats received celecoxib and safranal with ω‐3 HFD, respectively. GC–MS Gas chromatography Mass spectrometry was performed for analysis of fatty acid methyl ester. Enzyme‐linked immunosorbent assay was used to analyze serum interleukin‐6 (IL‐6) and transforming growth factor‐beta 1 (TGF‐β1) concentrations. A statistically significant decrease of AA‐to‐EPA ratio was observed in group VII when compared with the groups receiving HFDs. This group also showed the lowest serum IL‐6 level and highest TGF‐β1 level. In conclusion, ω3‐enriched diet along with drugs (e.g. celecoxib) and herbal medications (e.g. safranal) may have an anti‐inflammatory effect in lipotoxicity. GC–MS with single‐ion monitoring is valid for the analysis of FAs.

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“…Our previously randomized multicenter clinical trial has suggested that DMC exhibits an effective lipid‐lowering activity with low side effects . DMC can also improve heart function , reverse the impaired parasympathetic baroreflex in STZ‐induced diabetic rats with hyperlipidemia , and protect the endothelial dysfunction of aorta induced by high‐fat diet .…”

Section: Introductionmentioning

confidence: 99%

Plasma pharmacochemistry combined with pharmacokinetics and pattern recognition analysis to screen potentially bioactive components from Daming capsule using ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight mass spectrometry

et al. 2015

J of Separation Science

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Daming capsule is a traditional Chinese medicine for hyperlipidemia treatment. However, the vague understanding of the bioactive components of Daming capsule hampers its modernization and internationalization. This work first developed a high-throughput, high-resolution, and high-sensitivity ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry method for identifying the absorbed compounds and monitoring the pharmacokinetics of Daming capsule. A high-throughput strategy integrating plasma pharmacochemistry, pharmacokinetics, and pattern recognition analysis was also established for screening the bioactive components of Daming capsule in vivo. The established strategy based on ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry was successfully applied to screen the bioactive components of Daming capsule. Up to 53 absorbed compounds were identified. Six anthraquinones with fast and high absorption, namely, emodin-O-glucoside, aurantio-obtusin, aloe-emodin, rhein, emodin, and chrysophanol, were screened as potentially bioactive components of Daming capsule. The plasma pharmacochemistry and pharmacokinetics of Daming capsule were reported for the first time. Notably, the high-throughput and reliable strategy facilitated the screening and identification of bioactive components of traditional Chinese medicine, thereby providing novel insights into the research and development of new drugs.

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Daming capsule restores endothelial dysfunction induced by high-fat diet

Cited by 11 publications

References 27 publications

Protective effect of Daming capsule against chronic cerebral ischemia

Protective effect of Daming capsule against chronic cerebral ischemia

Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity

Plasma pharmacochemistry combined with pharmacokinetics and pattern recognition analysis to screen potentially bioactive components from Daming capsule using ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight mass spectrometry

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