Damages Induced in Nucleic Acids by Photosensitization

Piette, Jacques; Paule, Marie; Louis, Merville; Decuyper, J.

doi:10.1111/j.1751-1097.1986.tb05539.x

Cited by 66 publications

(30 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 O 2 is known to be the main ROS produced by photosensitization (56) be involved in the activation mechanism. However, the role of 1 O 2 as mediator in NF-B activation by PPME photosensitization could be considered unlikely because (i) none of the tested antioxidants (hydrophilic and hydrophobic) capable of quenching 1 O 2 inhibit NF-B activation and (ii) no lipoperoxide can be detected in photosensitized HCT-116 cells.…”

Section: Discussionmentioning

confidence: 99%

Pyropheophorbide-a Methyl Ester-mediated Photosensitization Activates Transcription Factor NF-κB through the Interleukin-1 Receptor-dependent Signaling Pathway

Matroule¹,

Bonizzi²,

Morlière³

et al. 1999

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Pyropheophorbide-a methyl ester (PPME) is a second generation of photosensitizers used in photodynamic therapy. We demonstrated that PPME photosensitization activated NF-B transcription factor in colon cancer cells. Unexpectedly, this activation occurred in two separate waves, i.e. a rapid and transient one and a second slower but sustained phase. The former was due to photosensitization by PPME localized in the cytoplasmic membrane which triggered interleukin-1 receptor internalization and the transduction pathways controlled by the interleukin-1 type I receptor. Indeed, TRAF6 dominant negative mutant abolished NF-B activation by PPME photosensitization, and TRAF2 dominant negative mutant was without any effect, and overexpression of IB kinases increased gene transcription controlled by NF-B. Oxidative stress was not likely involved in the activation. On the other hand, the slower and sustained wave could be the product of the release of ceramide through activation of the acidic sphingomyelinase. PPME localization within the lysosomal membrane could explain why ceramide acted as second messenger in NF-B activation by PPME photosensitization. These data will allow a better understanding of the molecular basis of tumor eradication by photodynamic therapy, in particular the importance of the host cell response in the treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Pyropheophorbide-a Methyl Ester-mediated Photosensitization Activates Transcription Factor NF-κB through the Interleukin-1 Receptor-dependent Signaling Pathway

Matroule¹,

Bonizzi²,

Morlière³

et al. 1999

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Inside cells, 1 O 2 can be genotoxic when generated in the vicinity of DNA, e.g. by irradiation in the presence of a hydrophilic photosensitizer such as RB or with photosensitizers having DNA affinity [47,48]. When 1 O 2 is generated outside cells, its primary target is the membrane where it can initiate lipid peroxidation [49] or protein oxidation at the level of aromatic residues [50].…”

Section: Discussionmentioning

confidence: 99%

HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen

Legrand-Poels¹,

Hoebeke²,

Vaira³

et al. 1993

Journal of Photochemistry and Photobiology B: Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…C, susceptibility of dGuo residues to damage by free singlet oxygen. The finding that blocking lesions at dGuo residues are also produced by another singlet-oxygen generator, proflavine and visible light (43), favors the latter explanation. In contrast, the argument for region-specific generation of singlet oxygen is favored by the observation that 53% of the single-base mutations occur at the G position in the sequence CGT.…”

mentioning

confidence: 84%

Mutations induced by methylene blue plus light in single-stranded M13mp2.

McBride

Schneider

Floyd

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Reactive oxygen species are generated by a variety of cellular processes. These endogenously generated, reactive intermediates produce a multiplicity of DNA alterations and mutations and have been implicated in the pathogenesis of several human diseases. We report here that treatment of single-stranded M13mp2 bacteriophage DNA with methylene blue and white light generates increased levels of 8-hydroxydeoxyguanosine and that mutagenesis is both highly specific and dependent on the SOS response. Lesions produced block the progression of DNA synthesis one base preceding template guanines. In SOS-induced Escherichia coli, 97% of all methylene blue-induced mutations in the lacZa gene of M13mp2 DNA are single-base substitutions opposite template guanines. The most frequent mutations are G -+ C transversions. The G -+ T transversions expected from the presence of 8-hydroxydeoxyguanosine in the template strand occur, but at a lower frequency. Sequence data together with SOS dependency and the presence of replication blockage demonstrate that while 8-hydroxydeoxyguanosine may serve as an important marker to monitor oxygen-induced DNA damage in humans, it does not account for either the observed blockage to replication or the mutagenesis by methylene blue plus light in SOS-induced E. coli. Instead, an as yet unidenti lesion generated by active oxygen species is a more potent mutagenic event.In cells, oxygen is metabolized by a series of one-electron reductions. A result ofoxygen metabolism is the formation of free radicals and related species including hydroxyl radicals, hydrogen peroxide, superoxide ions, and singlet oxygen. These highly reactive molecules can damage many cellular macromolecules, including proteins, lipids, RNA, and DNA (1, 2), and the resultant deleterious effects have been hypothesized to contribute to the pathogenesis of many agerelated human diseases, including cancer, atherosclerosis, and joint diseases (3, 4). To elucidate the relationship of DNA damage by reactive oxygen species to the pathogenesis of diseases, methods are required to quantitate the different types of DNA alterations produced and to determine the mutagenic potential of each alteration. However, the multiplicity and complexity of reactions that generate reactive oxygen species in cells have complicated these analyses. First, there are multiple processes in cells that generate oxygen free radicals (2, 5), and these radicals undergo further reactions to form additional reactive species. Second, based on the multiplicity of nucleotide alterations produced by oxygen free radicals in vitro, it can be estimated that in DNA more than 100 different nucleotide adducts can be formed (6-9). Third, certain molecules that generate oxygen free radicals preferentially bind to specific nucleotide sequences in DNA (10, 11). Fourth, long-lived DNA damage and mutagenesis result from inadequate DNA repair and altered incorporation by DNAreplicating enzymes, respectively; these mechanisms, in eukaryotic cells, have yet to be characterized (12)....

show abstract

Damages Induced in Nucleic Acids by Photosensitization

Cited by 66 publications

References 73 publications

Pyropheophorbide-a Methyl Ester-mediated Photosensitization Activates Transcription Factor NF-κB through the Interleukin-1 Receptor-dependent Signaling Pathway

Pyropheophorbide-a Methyl Ester-mediated Photosensitization Activates Transcription Factor NF-κB through the Interleukin-1 Receptor-dependent Signaling Pathway

HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen

Mutations induced by methylene blue plus light in single-stranded M13mp2.

Contact Info

Product

Resources

About