“…[14] These seminalr eports demonstrated that the photoinduced activation of ac aged fluorescein-dextran conjugate can be exploited to track engrailed expression in fixed and immunolabeled embryos and suggested the possible use of these photoresponsive constructs to probe cell dynamics in living organisms. These studies stimulated similari nvestigationsw ith caged coumarin, fluorescein, and rhodamine derivatives also in barley, [15] frog, [16] sea urchin, [17] worm, [18] and zebrafish [19][20][21][22][23][24][25] embryos. The poor photobleaching resistance of the emissive state of most of these photoactivatable probes, however, restricts the exposure of the activated species to fairly short times.…”