Damage initiation and progression in the cartilage surface probed by nonlinear optical microscopy

Brown, Cameron; Houle, Marie-Andrée; Chen, M.; Price, Andrew; Légaré, François; Gill, H. S.

doi:10.1016/j.jmbbm.2011.08.004

Cited by 32 publications

(27 citation statements)

References 48 publications

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“…The acquired signal reflects the localization and amount of fibrous structures of the collagen molecule. Therefore, it is proposed that SHG imaging enables the clinician to detect both quantitative and qualitative changes in the cartilage matrix at a microscopic resolution [6][7][8][9][10][11][12][13]. Several animal OA models, including histopathological assessments of their osteoarthritic processes, are well established [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application

Kiyomatsu

Oshima

Saitou

et al. 2015

Biomed. Opt. Express

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Osteoarthritis (OA) restricts the daily activities of patients and significantly decreases their quality of life. The development of noninvasive quantitative methods for properly diagnosing and evaluating the process of degeneration of articular cartilage due to OA is essential. Second harmonic generation (SHG) imaging enables the observation of collagen fibrils in live tissues or organs without staining. In the present study, we employed SHG imaging of the articular cartilage in OA model mice ex vivo. Consequently, three-dimensional SHG imaging with successive image processing and statistical analyses allowed us to successfully characterize histopathological changes in the articular cartilage consistently confirmed on histological analyses. The quantitative SHG imaging technique presented in this study constitutes a diagnostic application of this technology in the setting of OA.

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Section: Introductionmentioning

confidence: 99%

Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application

Kiyomatsu

Oshima

Saitou

et al. 2015

Biomed. Opt. Express

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show abstract

“…Jessica et al, demonstrated morphological changes in degenerative equine articular cartilage [12]. Brown et al, has investigated collagen meshwork architecture by a two-dimensional spring-mass network model in bovine high grade osteoarthritic cartilage [13]. Kiyomatsu et al, presented a quantitative approach to characterize induced OA in a mice model [14].…”

Section: Introductionmentioning

confidence: 99%

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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Abstract:In a synovial joint, the articular cartilage is directly affected during the progression of Osteoarthritis (OA). The characterization of early stage modification in extra-cellular matrix of cartilage is essential for detection as well as understanding the progression of disease. The objective of this study is to demonstrate the potential and capability of nonlinear optical microscopy for the morphological investigation of early stage osteoarthritic cartilage. ICRS Grade-I cartilage sections were obtained from the femoral condyle of the human knee. The surface of articular cartilage was imaged by second harmonic generation and two-photon excited fluorescence microscopy. Novel morphological features like microsplits and wrinkles were observed, which would otherwise not be visible in other clinical imaging modalities (e.g., CT, MRI, ultrasound and arthroscope. The presence of superficial layer with distinct collagen fibrils parallel to the articular surface in 4 specimens out of 14 specimens, indicates that different phases of OA within ICRS Grade-I can be detected by SHG microscopy. All together, the observed novel morphologies in early stage osteoarthritic cartilage indicates that SHG microscopy might be a significant tool for the assessment of cartilage disorder.

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“…While there have been a few efforts using SHG to image cartilage (29, 31), these have not yet exploited the full power of the modality in terms of extracting structure (16, 20). There is also limited data on optical properties.…”

Section: Discussionmentioning

confidence: 99%

Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy

Chaudhary¹,

Campbell

Tilbury

et al. 2015

Connective Tissue Research

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Purpose The collagen structure throughout the patella has not been thoroughly investigated by 3D imaging, where the majority of the exiting data comes from histological cross sections. It is important to have a better understanding of the architecture in normal tissues, where this could then be applied to imaging of diseased states. Methods To address this shortcoming, we investigated the combined use of collagen specific Second Harmonic Generation (SHG) imaging and measurement of bulk optical properties to characterize collagen fiber orientations of the histologically defined zones of bovine articular cartilage. Forward and backward SHG intensities from sections from superficial, middle and deep zones were collected as a function of depth and analyzed by Monte Carlo simulations to extract the SHG creation direction, which is related to the fibrillar assembly. Results Our results revealed differences in SHG forward-backward response between the three zones, where these are consistent with a previously developed model of SHG emission. Some of the findings are consistent with that from other modalities; however, SHG analysis showed the middle zone had the most organized fibril assembly. While not distinct, we also report bulk optical property values for these different zones within the patella. Conclusions Collectively, these results provide quantitative measurements of structural changes at both the fiber and fibril assembly of the different cartilage zones and reveals structural information not possible by other microscope modalities. This can provide quantitative insight to the collagen fiber network in normal cartilage, which may ultimately be developed as a biomarker for osteoarthritis.

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Damage initiation and progression in the cartilage surface probed by nonlinear optical microscopy

Cited by 32 publications

References 48 publications

Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application

Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy

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