Dalestones A and B, two anti-inflammatory cyclopentenones from Daldinia eschscholzii with an edited strong promoter for the global regulator LaeA-like gene

Zhang, Zhou; Zhu, Hongjie; Yang, Chenglong; Liu, Yanjun; Jiang, Nan; Xiao, Yongsheng; Shi, Liyun; Jiao, Rui‐Hua; Ge, Hui‐Ming; Tan, Renxiang

doi:10.1016/s1875-5364(19)30045-7

Cited by 3 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From medical plants, Shylaja et al [9] isolated D.eschscholtzii from Mussaenda luteola and purified compound 5-methoxy-2methyl-3-pentacosyl-1,4-benzoquinone (14). From the mangrove endophytic fungus D.eschscholtzii HJ001, Yang et al [10] obtained a new cytochalasin (15). And then, the group [11] isolated two new polyketides 8-O-methylnodulisporin F (16) and nodulisporin H (17), two new naphthoquinones, 5-hydroxy-2methoxy-6,7-dimethyl-1,4-naphthoquinone (18), and 5-hydroxy-2-methoxynaphtho [9-c] furan-1,4-dione (19), and a new naphthofuran 1,3,8-trimethoxynaphtho [9-c] furan (20).…”

Section: Natural Products Of Various Species Of the Genus Daldiniamentioning

confidence: 99%

“…And then, this group [14] found two skeletally undescribed polyketide-indole hybrids from the indole-3-carbinol-exposed culture of D. eschscholzii named indolchromins A and B (33-34). By replacement of the native promoter of the global regulator LaeA-like gene of D.eschscholzii by a strong gpdA promoter, Zhou et al [15] obtained two novel anti-inflammatory cyclopentenone metabolites named dalestones A and B (35-36). From metabolites of D.eschscholzii JC-15 cultured in red ginseng medium, Wang et al [16] obtained an unprecedented benzopyran-naphthalene hybrid daldinsin (37) and a new lactone, 8-hydroxylhelicascolide A (38).…”

Section: Minireviewmentioning

confidence: 99%

“…[7] Dalestones A and B (35-36) have anti-inflammatory activity and inhibit the gene expression of TNF-α and IL-6 in LPS-induced RAW264.7 macrophages. [15] Daldispones A and B (55-56) displayed significant anti-IAV (H1N1) activities with the IC50 values of 16.0 and 7.4 μM, respectively. [22] Daldinsin (37) showed anti-acetylcholinesterase activity with an inhibition ratio of 38.8% at 50 μM.…”

Section: Minireviewmentioning

confidence: 99%

See 2 more Smart Citations

Natural Products from the genus Daldinia and Their Bioactivities

2021

Med. Res.

View full text Add to dashboard Cite

Section: Natural Products Of Various Species Of the Genus Daldiniamentioning

confidence: 99%

Section: Minireviewmentioning

confidence: 99%

Section: Minireviewmentioning

confidence: 99%

See 1 more Smart Citation

Natural Products from the genus Daldinia and Their Bioactivities

2021

Med. Res.

View full text Add to dashboard Cite

Unveiling the potential of Daldinia eschscholtzii MFLUCC 19-0629 through bioactivity and bioinformatics studies for enhanced sustainable agriculture production

Brooks,

Weaver,

Klomchit

et al. 2024

Front. Chem. Biol.

View full text Add to dashboard Cite

Endophytic fungi constitute a rich source of secondary metabolites that can be manipulated to produce desirable novel analogs for combating current agricultural challenges for crop production, especially controlling plant disease. The endophytic fungus Daldinia eschscholtzii MFLUCC 19-0629, was newly isolated from tropical ancient plants, Oncosperma sp., and displays a broad-spectrum of antifungal and antibacterial activities against several plant pathogens including Ralstonia solanacearum, Fusarium oxysporum, Colletotrichum gloeosporioides, Colletotrichum acutatum, Stagonosporopsis cucurbitacearum, Corynespora cassiicola and Stemphylium spp. A high-quality genome sequence was obtained using Oxford nanopore technology, the accuracy and length of reads resulting in no need for Illumina or other sequencing techniques, for D. eschscholtzii MFLUCC 19-0629, resulting in a genome size of 37.56 Mb assembled over 11 contigs of significant size, likely to be at the chromosomal level. Bioinformatics analysis revealed that this strain is biosynthetically talented encoding 67 predicted biosynthetic gene clusters (BGCs). Only eight of the 67 BGCs matched or demonstrated high similarity to previously characterized BGCs linked to the production of known secondary metabolites. The high number of predicted unknown BGCs makes this strain a promising source of novel natural products. The discovery that D. eschscholtzii MFLUCC 19-0629 has a broad spectrum of antimicrobial activity against seven major plant pathogenic microorganisms relevant to crop production and its complete genome sequence carries immense importance in the advancement of novel microbial biocontrol agents (MBCAs). This also unveils the prospect of uncovering new compounds that could be utilized for sustainable agriculture and pharmaceutical purposes.

show abstract

Overexpression of llm1 Affects the Synthesis of Secondary Metabolites of Aspergillus cristatus

et al. 2022

View full text Add to dashboard Cite

Putative methyltransferases are thought to be involved in the regulation of secondary metabolites in filamentous fungi. Here, we report the effects of overexpression of a predicted LaeA-like methyltransferase gene llm1 on the synthesis of secondary metabolites in Aspergillus cristatus. Our results revealed that overexpression of the gene llm1 in A. cristatus significantly hindered the production of conidia and enhanced sexual development, and reduced oxidative tolerance to hydrogen peroxide. Compared with the wild-type, the metabolic profile of the overexpression transformant was distinct, and the contents of multiple secondary metabolites were markedly increased, mainly including terpenoids and flavonoids, such as (S)-olEuropeic acid, gibberellin A62, gibberellin A95, ovalitenone, PD 98059, and 1-isomangostin. A total of 600 significantly differentially expressed genes (DEGs) were identified utilizing transcriptome sequencing, and the DEGs were predominantly enriched in transmembrane transport and secondary metabolism-related biological processes. In summary, the strategy of overexpressing global secondary metabolite regulators successfully activated the expression of secondary metabolite gene clusters, and the numerous secondary metabolites were greatly strengthened in A. cristatus. This study provides new insights into the in-depth exploitation and utilization of novel secondary metabolites of A. cristatus.

show abstract

Dalestones A and B, two anti-inflammatory cyclopentenones from Daldinia eschscholzii with an edited strong promoter for the global regulator LaeA-like gene

Cited by 3 publications

References 26 publications

<p class="Author"> <p class="Title" style="text-indent:0mm;"> Natural Products from the genus <i>Daldinia</i> and Their Bioactivities

<p class="Author"> <p class="Title" style="text-indent:0mm;"> Natural Products from the genus <i>Daldinia</i> and Their Bioactivities

Unveiling the potential of Daldinia eschscholtzii MFLUCC 19-0629 through bioactivity and bioinformatics studies for enhanced sustainable agriculture production

Overexpression of llm1 Affects the Synthesis of Secondary Metabolites of Aspergillus cristatus

Contact Info

Product

Resources

About