DAL82, a second gene required for induction of allantoin system gene transcription in Saccharomyces cerevisiae

Abstract

Several highly inducible enzyme activities are required for the degradation of allantoin in Saccharomyces cerevisiae. Induction of these pathway enzymes has been shown to be regulated at transcription, and response to inducer is lost in dal81 and dal82/durM mutants. The similar phenotypes generated by da)81 and dal82 mutations prompted the question of whether they were allelic. We demonstrated that the DAL81 and DAL82 loci are distinct, unlinked genes situated on chromosomes IX and XIV. DAL82 gene expression did not respond to induction by the allantoin pathway inducer or to nitrogen catabolite repression. Expression was also not significantly affected by mutation of the daI80 locus. From the nucleotide sequence of the DAL82 gene, we deduced that it encodes a protein with a mass of 29,079 Da that may possess the structural motifs expected of a regulatory protein. This protein was shown to be required for the function mediated by the cis-acting upstream induction sequence situated in the 5'-flanking regions of the inducible allantoin pathway genes.

Upstream induction sequence, the cis-acting element required for response to the allantoin pathway inducer and enhancement of operation of the nitrogen-regulated upstream activation sequence in Saccharomyces cerevisiae

“…The DAL82 locus encodes a 29-kDa protein specifically required for DAL7 UIS function (17). The phenotype of dal82 mutations is identical to that of dal8J with an important exception; UGAI gene expression is not affected by loss of the DAL82 gene product (24).…”

“…Hence, DAL82 appears to be allantoin pathway specific. The deduced structure of DAL82 protein does not contain any of the motifs usually reported to be associated with DNA binding proteins except a coiled-coil motif (17). A first step towards elucidating the functions of DAL81 and DAL82 proteins and unraveling potential interactions of all proteins required to mediate induced allantoin system gene expression is careful identification of the cis-acting sites involved in the process.…”

“…4) prompts the question of their identity. Two candidate proteins are encoded by the DAL8J and DAL82 genes (1,2,17,23). Both of these genes have been demonstrated to be required for induction (1,17,23), and for the functioning of the UIS element rather than UASNTR (2,17).…”

“…Two candidate proteins are encoded by the DAL8J and DAL82 genes (1,2,17,23). Both of these genes have been demonstrated to be required for induction (1,17,23), and for the functioning of the UIS element rather than UASNTR (2,17). However, because of the potential interaction of proteins associated with the very closely spaced UAS and UIS sites, the obvious gel shift experiments involving mutant forms of the DAL8J and DAL82 proteins have not thus far been interpretable (19a).…”

Interplay between the transcription factors acting on the GATA- and GABA-responsive elements of Saccharomyces cerevisiae UGA promoters

“…In all these induction processes, Dal81 acts together with an inducer-specific protein; this specific factor is Uga3 in GABA-induction of UGA genes (André, 1990), Dal82/DurM in allophanateinduction of DUR and DAL genes (André & Jauniaux, 1990b;Jacobs et al, 1980;Olive et al, 1991) and Stp1 in amino acid induction of amino acid permease genes (AbdelSater et al, 2004;Boban & Ljungdahl, 2007;Iraqui et al, 1999).…”