India is globally known as an agricultural country where animal husbandry plays a vital role in the socioeconomic development of the people. India owns the highest number of cattle and buffaloes worldwide. Therefore, livestock plays a vital role in the national economy. The livestock census of 2019 revealed that in comparison to the National figure, Uttarakhand shares 0.96% cattle, 0.79% buffaloes, 0.38% sheep, 0.92% goats, 0.20% pigs, and 0.59% poultry in terms of livestock. The Badri cow is found only in the hill districts, currently known as the State Badri cow, and was earlier known as the Pahadi Gai. Badri cow is a small, cattle having various colors black, brown, and grey, with an average weight of 200-250 kg. Badri cows provide high-quality milk which is full of nutritional components. This cattle graze on various shrubs and herbs of the forest; therefore, the nutritional content of milk varies from other breeds. The present study was conducted in the Nainital and districts of Uttarakhand. The milk samples were collected in a sterile bottle, carried out in coolant packs to the laboratory, and preserved at -20℃ for further investigation. The parameters, i.e., Adulteration, Crude Protein, Fat, Total solid, Solid not fat, and Ash, were estimated. The present investigation yielded negative adulteration results with 1-4% Crude Protein, 1-8% fat, 11-17% total solid, 8-10% Solid Not Fat, and 0.1 -1% ash in all the milk samples. This preliminary study explains that Badri cow milk is used by people for their consumption, rarely for commercial purposes, and is an excellent source of a nutritional diet for infants.