Daily Variations of Homocysteine Concentration May Influence Methylation of DNA in Normal Healthy Individuals

Bönsch, Dominikus; Hothorn, Torsten; Krieglstein, C. F.; Koch, M; Nehmer, Christina; Lenz, Bernd; Reulbach, Udo; Kornhuber, Johannes; Bleich, Stefan

doi:10.1080/07420520701290565

Cited by 45 publications

(26 citation statements)

References 38 publications

(37 reference statements)

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“…In our data, LINE-1 and Alu methylation had associations with mean outdoor temperature of the day of the exam that, although not significant, were consistent with those observed with season. A previous study of 30 healthy volunteers reported cyclic variations in blood DNA methylation content observed over a 24-hour period (36), and recent investigations have shown transient cyclical methylation and demethylation of specific gene promoters (37,38). The association of LINE-1 methylation with day of the week observed in our data may provide further evidence for the existence of cyclical patterns of variation in repetitive element DNA methylation.…”

Section: Discussionsupporting

confidence: 70%

Rapid DNA Methylation Changes after Exposure to Traffic Particles

Baccarelli

Wright

Bollati

et al. 2009

Am J Respir Crit Care Med

592

451

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Rationale: Exposure to particulate air pollution has been related to increased hospitalization and death, particularly from cardiovascular disease. Lower blood DNA methylation content is found in processes related to cardiovascular outcomes, such as oxidative stress, aging, and atherosclerosis. Objectives: We evaluated whether particulate pollution modifies DNA methylation in heavily methylated sequences with high representation throughout the human genome. Methods: We measured DNA methylation of long interspersed nucleotide element (LINE)-1 and Alu repetitive elements by quantitative polymerase chain reaction-pyrosequencing of 1,097 blood samples from 718 elderly participants in the Boston area Normative Aging Study. We used covariate-adjusted mixed models to account for within-subject correlation in repeated measures. We estimated the effects on DNA methylation of ambient particulate pollutants (black carbon, particulate matter with aerodynamic diameter < 2.5 mm [PM 2.5 ], or sulfate) in multiple time windows (4 h to 7 d) before the examination. We estimated standardized regression coefficients (b) expressing the fraction of a standard deviation change in DNA methylation associated with a standard deviation increase in exposure. Measurements and Main Results: Repetitive element DNA methylation varied in association with time-related variables, such as day of the week and season. LINE-1 methylation decreased after recent exposure to higher black carbon (b 5 20.11; 95% confidence interval [CI], 20.18 to 20.04; P 5 0.002) and PM 2.5 (b 5 20.13; 95% CI, 20.19 to 20.06; P , 0.001 for the 7-d moving average). In two-pollutant models, only black carbon, a tracer of traffic particles, was significantly associated with LINE-1 methylation (b 5 20.09; 95% CI, 20.17 to 20.01; P 5 0.03). No association was found with Alu methylation (P . 0.12). Conclusions: We found decreased repeated-element methylation after exposure to traffic particles. Whether decreased methylation mediates exposure-related health effects remains to be determined.

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Section: Discussionsupporting

confidence: 70%

Rapid DNA Methylation Changes after Exposure to Traffic Particles

Baccarelli

Wright

Bollati

et al. 2009

Am J Respir Crit Care Med

592

451

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“…A previous study that assessed global DNA methylation by fluorescently labeled cytosine extension assay reported diurnal changes in global DNA methylation in humal blood. 50 The present study is the first to demonstrate a diurnal rhythm of global DNA methylation in the liver. Furthermore, the variation appeared to be coordinated with the diurnal variation in Dnmt3b expression.…”

Section: Discussionmentioning

confidence: 98%

Diurnal expression ofDnmt3bmRNA in mouse liver is regulated by feeding and hepatic clockwork

et al. 2012

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“…In humans, several peptide hormone levels show daily variations; growth hormone increases during sleep (19), leptin increases during the evening (20), and prolactin increases at night (21). Concentrations of amino acids, including tryptophan, tyrosine, phenylalanine (22), methionine (23), cysteine, glutathione (24), and homocysteine (25), also exhibit daily variations in human blood plasma. Despite these findings, comprehensive profiling of circadian dynamics of chemical substances in mammalian blood has not yet been reported, and until now a comprehensive molecular timetable of such chemical substances has not been constructed.…”

mentioning

confidence: 99%

Measurement of internal body time by blood metabolomics

Minami

Kasukawa²,

Kakazu

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

243

177

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Detection of internal body time (BT) via a few-time-point assay has been a longstanding challenge in medicine, because BT information can be exploited to maximize potency and minimize toxicity during drug administration and thus will enable highly optimized medication. To address this challenge, we previously developed the concept, ''molecular-timetable method,'' which was originally inspired by Linné 's flower clock. In Linné 's flower clock, one can estimate the time of the day by watching the opening and closing pattern of various flowers. Similarly, in the molecular-timetable method, one can measure the BT of the day by profiling the up and down patterns of substances in the molecular timetable. To make this method clinically feasible, we now performed blood metabolome analysis and here report the successful quantification of hundreds of clock-controlled metabolites in mouse plasma. Based on circadian blood metabolomics, we can detect individual BT under various conditions, demonstrating its robustness against genetic background, sex, age, and feeding differences. The power of this method is also demonstrated by the sensitive and accurate detection of circadian rhythm disorder in jet-lagged mice. These results suggest the potential for metabolomics-based detection of BT (''metabolite-timetable method''), which will lead to the realization of chronotherapy and personalized medicine.chronotherapy ͉ circadian ͉ metabolome ͉ jet-lag ͉ LC-MS

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Daily Variations of Homocysteine Concentration May Influence Methylation of DNA in Normal Healthy Individuals

Cited by 45 publications

References 38 publications

Rapid DNA Methylation Changes after Exposure to Traffic Particles

Rapid DNA Methylation Changes after Exposure to Traffic Particles

Diurnal expression ofDnmt3bmRNA in mouse liver is regulated by feeding and hepatic clockwork

Measurement of internal body time by blood metabolomics

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