Daily thanatomicrobiome changes in soil as an approach of postmortem interval estimation: An ecological perspective

Adserias‐Garriga, Joe; Hernández, Marta; Quijada, Narciso M.; Lázaro, David Rodríguez; Steadman, Dawnie Wolfe; Garcia‐Gil, L. J.

doi:10.1016/j.forsciint.2017.07.017

Cited by 53 publications

(57 citation statements)

References 38 publications

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“…Thanatomicrobiome studies have determined that there is extremely rapid postmortem overgrowth of Clostridium spp. within decaying internal body sites (e.g., blood, bone marrow, liver, prostate) ( Clement et al, 2016 ; Javan et al, 2016a , b , Adserias-Garriga et al, 2017a , b ; Thomas et al, 2017 ; Zhao et al, 2017 ). Human decomposition is a multifactorial process mediated by microbes, which inhabit, proliferate, and die externally and internally throughout dead biomass ( Javan et al, 2016a , b ).…”

Section: Introductionmentioning

confidence: 99%

Cadaver Thanatomicrobiome Signatures: The Ubiquitous Nature of Clostridium Species in Human Decomposition

et al. 2017

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Human thanatomicrobiome studies have established that an abundant number of putrefactive bacteria within internal organs of decaying bodies are obligate anaerobes, Clostridium spp. These microorganisms have been implicated as etiological agents in potentially life-threatening infections; notwithstanding, the scale and trajectory of these microbes after death have not been elucidated. We performed phylogenetic surveys of thanatomicrobiome signatures of cadavers’ internal organs to compare the microbial diversity between the 16S rRNA gene V4 hypervariable region and V3-4 conjoined regions from livers and spleens of 45 cadavers undergoing forensic microbiological studies. Phylogenetic analyses of 16S rRNA gene sequences revealed that the V4 region had a significantly higher mean Chao1 richness within the total microbiome data. Permutational multivariate analysis of variance statistical tests, based on unweighted UniFrac distances, demonstrated that taxa compositions were significantly different between V4 and V3-4 hypervariable regions (p < 0.001). Of note, we present the first study, using the largest cohort of criminal cases to date, that two hypervariable regions show discriminatory power for human postmortem microbial diversity. In conclusion, here we propose the impact of hypervariable region selection for the 16S rRNA gene in differentiating thanatomicrobiomic profiles to provide empirical data to explain a unique concept, the Postmortem Clostridium Effect.

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Section: Introductionmentioning

confidence: 99%

Cadaver Thanatomicrobiome Signatures: The Ubiquitous Nature of Clostridium Species in Human Decomposition

et al. 2017

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“…In one study, the migration of microbes from body sites into body fluid and the subsequent translocation of these microbes were observed in at least 21 bacterial genera [ 60 ]. Most recently, it has been demonstrated that Firmicutes sharply rise in abundance in the soil around the cadaver [ 11 ] because bacteria in the body are transferred to the soil, similar to what has been observed for the thanatomicrobiome. In addition, fly-associated taxa such as Ignatzschineria and Wohlfahrtiimonas have been shown to expand in postmortem gut microbiome [ 54 ], suggesting for the importance of environment colonization in thanatomicrobiome succession.…”

Section: Community Characteristics Of Thanatomicrobiomementioning

confidence: 68%

“…At present, many studies have used animal models such as rodents and swine [ 49–51 ], others used donated human bodies or bodies from criminal cases [ 11 , 52–55 ]. The main concern of using animal models is whether the system is similar enough to human bodies, although it has been shown that swine is similar to human in terms of the body decomposition process [ 56 ].…”

Section: Community Characteristics Of Thanatomicrobiomementioning

confidence: 99%

“…[ 68 ], changes of soil microbial communities around human cadavers include distinct alteration in community composition (from a community abundant in Firmicutes and Proteobacteria to a community dominated by Firmicutes ), but not overall species abundances [ 68 ]. Quantitatively, the growth curve of Firmicutes in soil samples around human cadavers were shown to be able to predict PMI during Tennessee summer conditions [ 11 ], although the generality of the predictive model requires further studies.…”

Section: Community Characteristics Of Thanatomicrobiomementioning

confidence: 99%

“…The microbial community associated with the host after death is referred to as thanatomicrobiome [ 10 ], named after Thanatos, the representation of death in Greek mythology. Upon death, host tissues and cells decompose and cellular components are released to the surrounding tissues, resulting in significant changes in the host environment overtime [ 11–13 ]. Such changes shape, and are influenced by, host microbes as well as environmental microbes, resulting in characteristic microbial community dynamics specific to thanatomicrobiome.…”

Section: Introductionmentioning

confidence: 99%

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Thanatomicrobiome composition profiling as a tool for forensic investigation

Zhou

Bian

2018

Forensic Sciences Research

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Thanatomicrobiome, or the postmortem microbiome, has been recognized as a useful microbial marker of the time and location of host death. In this mini-review, we compare the experimental methods commonly applied to thanatomicrobiome studies to the state-of-the-art methodologies in the microbiome field. Then, we review present findings in thanatomicrobiome studies, focusing on the diversity of the thanatomicrobiome composition and prediction models that have been proposed. Finally, we discuss potential improvements and future directions of the field.

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From flesh to bones: Multi‐omics approaches in forensic science

Procopio,

Bonicelli

2024

Proteomics

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Recent advancements in omics techniques have revolutionised the study of biological systems, enabling the generation of high‐throughput biomolecular data. These innovations have found diverse applications, ranging from personalised medicine to forensic sciences. While the investigation of multiple aspects of cells, tissues or entire organisms through the integration of various omics approaches (such as genomics, epigenomics, metagenomics, transcriptomics, proteomics and metabolomics) has already been established in fields like biomedicine and cancer biology, its full potential in forensic sciences remains only partially explored. In this review, we have presented a comprehensive overview of state‐of‐the‐art analytical platforms employed in omics research, with specific emphasis on their application in the forensic field for the identification of the cadaver and the cause of death. Moreover, we have conducted a critical analysis of the computational integration of omics approaches, and highlighted the latest advancements in employing multi‐omics techniques for forensic investigations.

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Daily thanatomicrobiome changes in soil as an approach of postmortem interval estimation: An ecological perspective

Cited by 53 publications

References 38 publications

Cadaver Thanatomicrobiome Signatures: The Ubiquitous Nature of Clostridium Species in Human Decomposition

Cadaver Thanatomicrobiome Signatures: The Ubiquitous Nature of Clostridium Species in Human Decomposition

Thanatomicrobiome composition profiling as a tool for forensic investigation

From flesh to bones: Multi‐omics approaches in forensic science

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